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Home / Equine Research Bank / Cells / Allogeneic Serum and Macromolecular Crowding Maintain Native...
Cells2022; 11(9); 1562; doi: 10.3390/cells11091562

Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.

Abstract: The absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture. Herein, we assessed the influence of different sera (equine serum and foetal bovine serum) on equine tenocyte morphology, viability, metabolic activity, proliferation and protein synthesis as a function of tissue-specific extracellular matrix deposition (induced via macromolecular crowding), aging (passages 3, 6, 9) and time in culture (days 3, 5, 7). In comparison to cells at passage 3, at day 3, in foetal bovine serum and without macromolecular crowding (traditional equine tenocyte culture), the highest number of significantly decreased readouts were observed for cells in foetal bovine serum, at passage 3, at day 5 and day 7 and without macromolecular crowding. Again, in comparison to traditional equine tenocyte culture, the highest number of significantly increased readouts were observed for cells in equine serum, at passage 3 and passage 6, at day 7 and with macromolecular crowding. Our data advocate the use of an allogeneic serum and tissue-specific extracellular matrix for effective expansion of equine tenocytes.
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Publication Date: 2022-05-05 PubMed ID: 35563866PubMed Central: PMC9103545DOI: 10.3390/cells11091562Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research demonstrates that using allogeneic serum and tissue-specific extracellular matrix help maintain the functional properties of equine tenocytes (tendon cells in horses) in a lab culture. Specific comparison is made between the effects of equine serum and foetal bovine serum on tenocyte function.

Background and Objective

  • The study was driven by the problems often encountered during in vitro culture of tenocytes, predominantly the rapid loss of tenocyte function due to absence of a native extracellular matrix and the use of xenogeneic sera.
  • The research aimed to evaluate the impact of different types of serum (specifically, equine serum and foetal bovine serum) on equine tenocyte function. They investigated aspects such as morphological alterations, cell viability, metabolic activity, cell proliferation, and protein synthesis as influenced by the type of serum utilized.
  • Other factors considered were the amount of tissue-specific extracellular matrix deposition, cell culture ageing (assessed through different passages), and the time spent in culture.

Main Findings

  • The use of foetal bovine serum in culture without macromolecular crowding led to a significant decrease in a number of cellular readouts, including morphology, viability, metabolic activity, and protein synthesis. This was especially noticeable by the third passage, on days five and seven of the cultures.
  • Conversely, using equine serum for culture and applying macromolecular crowding (to stimulate tissue-specific extracellular matrix deposition) resulted in a significant increase in cellular readouts, predominantly by the third and sixth passages, notably by the seventh day in culture.

Conclusions and Implications

  • Data obtained in the study supports the use of allogeneic (or same-species) serum and tissue-specific extracellular matrix for maintaining tenocyte function in culture.
  • This finding has potential implications for the biomedical field, particularly in developing effective methods for expansion and transplantation of equine tenocytes, which might benefit clinical treatments of tendon injuries in horses.

Cite This Article

APA
Rampin A, Skoufos I, Raghunath M, Tzora A, Diakakis N, Prassinos N, Zeugolis DI. (2022). Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture. Cells, 11(9), 1562. https://doi.org/10.3390/cells11091562

Publication

Cells
ISSN: 2073-4409
NlmUniqueID: 101600052
Country: Switzerland
Language: English
Volume: 11
Issue: 9
PII: 1562

Researcher Affiliations

Rampin, Andrea
  • Laboratory of Animal Science, Nutrition and Biotechnology, School of Agriculture, University of Ioannina, 47100 Arta, Greece.
  • School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Charles Institute of Dermatology, Conway Institute of Biomolecular & Biomedical Research, School of Mechanical & Materials Engineering, University College Dublin (UCD), D04 V1W8 Dublin, Ireland.
Skoufos, Ioannis
  • Laboratory of Animal Science, Nutrition and Biotechnology, School of Agriculture, University of Ioannina, 47100 Arta, Greece.
Raghunath, Michael
  • Center for Cell Biology and Tissue Engineering, Institute for Chemistry and Biotechnology, Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland.
Tzora, Athina
  • Laboratory of Animal Science, Nutrition and Biotechnology, School of Agriculture, University of Ioannina, 47100 Arta, Greece.
Diakakis, Nikolaos
  • School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Prassinos, Nikitas
  • School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Zeugolis, Dimitrios I
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Charles Institute of Dermatology, Conway Institute of Biomolecular & Biomedical Research, School of Mechanical & Materials Engineering, University College Dublin (UCD), D04 V1W8 Dublin, Ireland.

MeSH Terms

  • Animals
  • Extracellular Matrix / metabolism
  • Hematopoietic Stem Cell Transplantation
  • Horses
  • Macromolecular Substances / metabolism
  • Serum Albumin, Bovine / metabolism
  • Tenocytes

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 6 times.
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