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Home / Equine Research Bank / Equine Vet J / Allogeneic vs. autologous intra-articular mesenchymal stem c...
Equine veterinary journal2019; 52(1); 144-151; doi: 10.1111/evj.13136

Allogeneic vs. autologous intra-articular mesenchymal stem cell injection within normal horses: Clinical and cytological comparisons suggest safety.

Abstract: Allogeneic bone marrow-derived mesenchymal stem cells (BMDMSCs) could provide multiple advantages over autologous BMDMSCs, including creating an 'off-the-shelf' treatment together with the ability to control for donor variation. Objective: The objective of the study was to compare the clinical and synovial fluid response of the normal equine joint to autologous and pooled-allogeneic BMDMSCs while controlling for individual variation and joint variations in response to intra-articular injections. We hypothesised that, by controlling for individual animal and joint variation, we could identify differences between allogeneic vs. autologous BMDMSCs in noninflamed joints. Methods: Randomised-controlled experiment. Methods: Bone marrow was harvested from eight horses. Autologous BMDMSCs were culture expanded, cryopreserved and thawed immediately prior to administration. For allogeneic BMDMSC treatments, four horses' BMDMSCs were culture expanded, pooled, cryopreserved and thawed immediately prior to use. Ten million (autologous or pooled-allogeneic) BMDMSCs were administered into contralateral forelimb metacarpophalangeal joints so that every autologous and allogeneic injection could be compared within the same animal. Clinical parameters included subjective lameness, objective lameness (Lameness Locator™), response to flexion, joint circumference and joint effusion. Arthrocentesis was performed for assessment of the nucleated cell count, differential cell count, total protein, and synovial concentrations of prostaglandin E2 (PGE2) and c-reactive protein (CRP). All parameters were measured at baseline, 6, 12, 24, 72, 168 and 336 h post-injection. Results: No difference was detected in any parameters between forelimb metacarpophalangeal joints administered autologous or pooled-allogeneic BMDMSCs. Conclusions: This study did not attempt to measure efficacy of BMDMSCs for musculoskeletal disease and should be followed by properly controlled efficacy trials. Conclusions: The study did not identify any clinical or cytological differences in the normal joint response to allogeneic or autologous BMDMSCs. A larger study to prove equivalence is warranted as allogeneic BMDMSCs may be a feasible alternative to autologous BMDMSCs.
© 2019 EVJ Ltd.
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Publication Date: 2019-06-24 PubMed ID: 31120574DOI: 10.1111/evj.13136Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial
  • Veterinary

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study aims to compare the effects of autologous and allogeneic bone marrow-derived mesenchymal stem cells (BMDMSCs) when injected into noninflamed joints of normal horses. The results show no significant clinical or cytological differences, suggesting allogeneic BMDMSCs may be a viable alternative to autologous BMDMSCs.

Objective and Hypothesis of the Study

  • The primary objective of this research was to compare the response of a normal horse’s joint to autologous (self-derived) and allogeneic (derived from a different individual) BMDMSCs.
  • The researchers controlled the conditions to account for individual and joint variations, with the hypothesis that this would enable them to spot any differences in the joint responses to the two types of cells in noninflamed joints.

Methodology

  • Bone marrow was harvested from eight horses.
  • For autologous treatment, BMDMSCs were cultured, preserved at low temperature (cryopreserved), and thawed right before administration.
  • For allogeneic treatments, BMDMSCs from four horses were cultured, combined, cryopreserved, and thawed just before use.
  • The injections were made into contralateral forelimb metacarpophalangeal joints, allowing researchers to compare the effects of both treatments within the same horse.
  • Clinical symptoms were evaluated, including both subjective and objective lameness, response to flexion, joint circumference, etc. Furthermore, arthrocentesis was performed to assess several cellular and protein-related variables.
  • These parameters were measured at multiple time points: before the treatment and 6, 12, 24, 72, 168, and 336 hours afterward.

Findings

  • The researchers noted no significant differences in any of the measured parameters between the joints that received autologous or allogeneic BMDMSCs.
  • The authors clarified that their study was not designed to assess the effectiveness of BMDMSCs for treating musculoskeletal diseases, which would require separate controlled trials.

Conclusion

  • The study concluded that there were no noticeable differences in joint responses to autologous or allogeneic BMDMSCs in the tested normal horses.
  • The authors suggested a larger study to confirm their findings and indicated that allogeneic BMDMSCs could potentially be a practical alternative to autologous BMDMSCs.

Cite This Article

APA
Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. (2019). Allogeneic vs. autologous intra-articular mesenchymal stem cell injection within normal horses: Clinical and cytological comparisons suggest safety. Equine Vet J, 52(1), 144-151. https://doi.org/10.1111/evj.13136

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 52
Issue: 1
Pages: 144-151

Researcher Affiliations

Colbath, A C
  • Orthopaedic Research Center, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.
Dow, S W
  • Department of Clinical Sciences, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.
Hopkins, L S
  • Department of Clinical Sciences, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.
Phillips, J N
  • Orthopaedic Research Center, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.
McIlwraith, C W
  • Orthopaedic Research Center, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.
Goodrich, L R
  • Orthopaedic Research Center, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.
  • Department of Clinical Sciences, Colorado State University, School of Veterinary Medicine, Fort Collins, Colorado, USA.

MeSH Terms

  • Animals
  • Biomarkers / chemistry
  • Bone Marrow Cells
  • Horses
  • Injections, Intra-Articular
  • Mesenchymal Stem Cell Transplantation / adverse effects
  • Mesenchymal Stem Cell Transplantation / veterinary
  • Mesenchymal Stem Cells / physiology
  • Synovial Fluid
  • Transplantation, Autologous
  • Transplantation, Homologous

Grant Funding

  • Wayne and Nancy McIlwraith Fellowship
  • Grayson Jockey Club Research Foundation
  • NIH Research Service Award

References

This article includes 50 references
  1. Beerts C, Suls M, Broeckx SY, Seys B, Vandenberghe A, Declercq J, Duchateau L, Vidal MA, Spaas JH. Tenogenically induced allogeneic peripheral blood mesenchymal stem cells in allogeneic platelet-rich plasma: 2-year follow-up after tendon or ligament treatment in horses. Front. Vet. Sci. 2017 4, 158.
  2. Broeckx S, Suls M, Beerts C, Vandenberghe A, Seys B, Wuertz-Kozak K, Duchateau L, Spaas JH. Allogenic mesenchymal stem cells as a treatment for equine degenerative joint disease: a pilot study. Curr. Stem Cell Res. Ther. 2014 9, 497-503.
  3. Vandenberghe A, Broeckx SY, Beerts C, Seys B, Zimmerman M, Verweire I, Suls M, Spaas JH. Tenogenically induced allogeneic mesenchymal stem cells for the treatment of proximal suspensory ligament desmitis in a horse. Front. Vet. Sci. 2015 2, 49.
  4. Textor JA, Clark KC, Walker NJ, Aristizobal FA, Kol A, LeJeune SS, Bledsoe A, Davidyan A, Gray SN, Bohannon-Worsley LK, Woolard KD, Borjesson DL. Allogeneic stem cells alter gene expression and improve healing of distal limb wounds in horses. Stem Cells Transl. Med. 2018 7, 98-108.
  5. Frisbie DD, Kisiday JD, Kawcak CE, Werpy NM, McIlwraith CW. Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. J. Orthop. Res. 2009 27, 1675-1680.
  6. Reisbig NA, Hussein HA, Pinnell E, Bertone AL. Evaluation of equine synovial-derived extracellular matrix scaffolds seeded with equine synovial-derived mesenchymal stem cells. Am. J. Vet. Res. 2018 79, 124-133.
  7. Carrade Holt DD, Wood JA, Granick JL, Walker NJ, Clark KC, Borjesson DL. Equine mesenchymal stem cells inhibit T cell proliferation through different mechanisms depending on tissue source. Stem Cells Dev. 2014 23, 1258-1265.
  8. Williams LB, Koenig JB, Black B, Gibson TW, Sharif S, Koch TG. Equine allogeneic umbilical cord blood derived mesenchymal stromal cells reduce synovial fluid nucleated cell count and induce mild self-limiting inflammation when evaluated in an lipopolysaccharide induced synovitis model. Equine Vet. J. 2016 48, 619-625.
  9. Colbath AC, Dow SW, Phillips JN, McIlwraith CW, Goodrich LR. Autologous and allogeneic equine mesenchymal stem cells exhibit equivalent immunomodulatory properties in vitro. Stem Cells Dev. 2017 26, 503-511.
  10. Paterson YZ, Rash N, Garvican ER, Paillot R, Guest DJ. Equine mesenchymal stromal cells and embryo-derived stem cells are immune privileged in vitro. Stem Cell Res. Ther. 2014 5, 90.
  11. Johnson V, Webb T, Norman A, Coy J, Kurihara J, Regan D, Dow S. Activated mesenchymal stem cells interact with antibiotics and host innate immune responses to control chronic bacterial infections. Sci. Rep. 2017 7, 9575.
  12. Kasashima Y, Ueno T, Tomita A, Goodship AE, Smith RK. Optimisation of bone marrow aspiration from the equine sternum for the safe recovery of mesenchymal stem cells. Equine Vet. J. 2011 43, 288-294.
  13. Pezzanite LM, Fortier LA, Antczak DF, Cassano JM, Brosnahan MM, Miller D, Schnabel LV. Equine allogeneic bone marrow-derived mesenchymal stromal cells elicit antibody responses in vivo. Stem Cell Res. Ther. 2015 6, 54.
  14. Schnabel LV, Pezzanite LM, Antczak DF, Felippe MJ, Fortier LA. Equine bone marrow-derived mesenchymal stromal cells are heterogeneous in MHC class II expression and capable of inciting an immune response in vitro. Stem Cell Res. Ther. 2014 5, 13.
  15. Carter-Arnold JL, Neilsen NL, Amelse LL, Odoi A, Dhar MS. In vitro analysis of equine, bone marrow-derived mesenchymal stem cells demonstrates differences within age- and gender-matched horses. Equine Vet. J. 2014 46, 589-595.
  16. Choudhery MS, Khan M, Mahmood R, Mehmood A, Khan SN, Riazuddin S. Bone marrow derived mesenchymal stem cells from aged mice have reduced wound healing, angiogenesis, proliferation and anti-apoptosis capabilities. Cell Biol. Int. 2012 36, 747-753.
  17. Kizilay Mancini O, Lora M, Shum-Tim D, Nadeau S, Rodier F, Colmegna I. A proinflammatory secretome mediates the impaired immunopotency of human mesenchymal stromal cells in elderly patients with atherosclerosis. Stem Cells Transl. Med. 2017 6, 1132-1140.
  18. Joswig AJ, Mitchell A, Cummings KJ, Levine GJ, Gregory CA, Smith R, Watts AE. Repeated intra-articular injection of allogeneic mesenchymal stem cells causes an adverse response compared to autologous cells in the equine model. Stem Cell Res. Ther. 2017 8, 42.
  19. Broeckx S, Spaas JH, Chiers K, Duchateau L, Van Hecke L, Van Brantegem L, Dumoulin M, Martens AM, Pille F. Equine allogeneic chondrogenic induced mesenchymal stem cells: a GCP target animal safety and biodistribution. Res. Vet. Sci. 2018 117, 246-254.
  20. Wang Y, Shimmin A, Ghosh P, Marks P, Linklater J, Connell D, Hall S, Skerrett D, Itescu S, Cicuttini FM. Safety, tolerability, clinical, and joint structural outcomes of a single intra-articular injection of allogeneic mesenchymal precursor cells in patients following anterior cruciate ligament reconstruction: a controlled double-blind randomised trial. Arthritis Res. Ther. 2017 19, 180.
  21. Broeckx S, Zimmerman M, Crocetti S, Suls M, Marien T, Ferguson SJ, Chiers K, Duchateau L, Franco-Obregon A, Wuertz K, Spaas JH. Regenerative therapies for equine degenerative joint disease: a preliminary study. PLoS One 2014 9, e85917.
  22. Owens SD, Kol A, Walker NJ, Borjesson DL. Allogeneic mesenchymal stem cell treatment induces specific alloantibodies in horses. Stem Cells Int. 2016 2016, 5830103.
  23. Liang C, Jiang E, Yao J, Wang M, Chen S, Zhou Z, Zhai W, Ma Q, Feng S, Han M. Interferon-gamma mediates the immunosuppression of bone marrow mesenchymal stem cells on T-lymphocytes in vitro. Hematology 2018 23, 44-49.
  24. Usha Shalini P, Vidyasagar JV, Kona LK, Ponnana M, Chelluri LK. In vitro allogeneic immune cell response to mesenchymal stromal cells derived from human adipose in patients with rheumatoid arthritis. Cell Immunol. 2017 314, 18-25.
  25. Pigott JH, Ishihara A, Wellman ML, Russell DS, Bertone AL. Investigation of the immune response to autologous, allogeneic, and xenogeneic mesenchymal stem cells after intra-articular injection in horses. Vet. Immunol. Immunopathol. 2013 156, 99-106.
  26. Ferris DJ, Frisbie DD, Kisiday JD, McIlwraith CW, Hague BA, Major MD, Schneider RK, Zubrod CJ, Kawcak CE, Goodrich LR. Clinical outcome after intra-articular administration of bone marrow derived mesenchymal stem cells in 33 horses with stifle injury. Vet. Surg. 2014 43, 255-265.
  27. Carmalt JL, Bell CD, Tatarniuk DM, Suri SS, Singh B, Waldner C. Comparison of the response to experimentally induced short-term inflammation in the temporomandibular and metacarpophalangeal joints of horses. Am. J. Vet. Res. 2011 72, 1586-1591.
  28. Ardanaz N, Vazquez FJ, Romero A, Remacha AR, Barrachina L, Sanz A, Ranera B, Vitoria A, Albareda J, Prades M, Zaragoza P, Martin-Burriel I, Rodellar C. Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints. BMC Vet. Res. 2016 12, 65.
  29. Mokbel AN, El Tookhy OS, Shamaa AA, Rashed LA, Sabry D, El Sayed AM. Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model. BMC Musculoskelet. Disord. 2011 12, 259.
  30. Textor JA, Willits NH, Tablin F. Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. Vet. J. 2013 198, 217-223.
  31. Ross TN, Kisiday JD, Hess T, McIlwraith CW. Evaluation of the inflammatory response in experimentally induced synovitis in the horse: a comparison of recombinant equine interleukin 1 beta and lipopolysaccharide. Osteoarthritis Cartilage 2012 20, 1583-1590.
  32. Frisbie DD, McIlwraith CW, Kawcak CE, Werpy NM. Evaluation of intra-articular hyaluronan, sodium chondroitin sulfate and N-acetyl-D-glucosamine combination versus saline (0.9% NaCl) for osteoarthritis using an equine model. Vet. J. 2013 197, 824-829.
  33. Frisbie DD, McIlwraith CW, Kawcak CE, Werpy NM. Efficacy of intravenous administration of hyaluronan, sodium chondroitin sulfate, and N-acetyl-d-glucosamine for prevention or treatment of osteoarthritis in horses. Am. J. Vet. Res. 2016 77, 1064-1070.
  34. Nelson BB, King MR, Frisbie DD. Assessment of a novel equine tarsocrural experimental joint disease model using recombinant interleukin-1beta and arthroscopic articular sampling of the medial malleolus of the tibia on the standing sedated horse. Vet. J. 2017 229, 54-59.
  35. King MR, Haussler KK, Kawcak CE, McIlwraith CW, Reiser RF, Frisbie DD, Werpy NM. Biomechanical and histologic evaluation of the effects of underwater treadmill exercise on horses with experimentally induced osteoarthritis of the middle carpal joint. Am. J. Vet. Res. 2017 78, 558-569.
  36. Mitchell A, Wright G, Sampson SN, Martin M, Cummings K, Gaddy D, Watts AE. Clodronate improves lameness in horses without changing bone turnover markers. Equine Vet. J. 2018 51, 356-363.
  37. Keegan KG, Yonezawa Y, Pai PF, Wilson DA, Kramer J. Evaluation of a sensor-based system of motion analysis for detection and quantification of forelimb and hind limb lameness in horses. Am. J. Vet. Res. 2004 65, 665-670.
  38. Burk J, Berner D, Brehm W, Hillmann A, Horstmeier C, Josten C, Paebst F, Rossi G, Schubert S, Ahrberg AB. Long-term cell tracking following local injection of mesenchymal stromal cells in the equine model of induced tendon disease. Cell Transplant. 2016 25, 2199-2211.
  39. Geburek F, Mundle K, Conrad S, Hellige M, Walliser U, van Schie HT, van Weeren R, Skutella T, Stadler PM. Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions-a pilot study. Stem Cell Res. Ther. 2016 7, 21.
  40. Park YB, Ha CW, Kim JA, Han WJ, Rhim JH, Lee HJ, Kim KJ, Park YG, Chung JY. Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel composite. Osteoarthritis Cartilage 2017 25, 570-580.
  41. Ikuta Y, Kamei N, Ishikawa M, Adachi N, Ochi M. In vivo kinetics of mesenchymal stem cells transplanted into the knee joint in a rat model using a novel magnetic method of localization. Clin. Transl. Sci. 2015 8, 467-474.
  42. Delling U, Brehm W, Metzger M, Ludewig E, Winter K, Julke H. In vivo tracking and fate of intra-articularly injected superparamagnetic iron oxide particle-labeled multipotent stromal cells in an ovine model of osteoarthritis. Cell Transplant. 2015 24, 2379-2390.
  43. Li M, Luo X, Lv X, Liu V, Zhao G, Zhang X, Cao W, Wang R, Wang W. In vivo human adipose-derived mesenchymal stem cell tracking after intra-articular delivery in a rat osteoarthritis model. Stem Cell Res. Ther. 2016 7, 160.
  44. Mancheno-Corvo P, Menta R, del Rio B, Franquesa M, Ramirez C, Hoogduijn MJ, DelaRosa O, Dalemans W, Lombardo E. T lymphocyte prestimulation impairs in a time-dependent manner the capacity of adipose mesenchymal stem cells to inhibit proliferation: role of interferon gamma, poly I:C, and tryptophan metabolism in restoring adipose mesenchymal stem cell inhibitory effect. Stem Cells Dev. 2015 24, 2158-2170.
  45. Duan W, Lopez MJ. Effects of cryopreservation on canine multipotent stromal cells from subcutaneous and infrapatellar adipose tissue. Stem Cell Rev. 2016 12, 257-268.
  46. James AW, Levi B, Nelson ER, Peng M, Commons GW, Lee M, Wu B, Longaker MT. Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo. Stem Cells Dev. 2011 20, 427-439.
  47. Ock SA, Rho GJ. Effect of dimethyl sulfoxide (DMSO) on cryopreservation of porcine mesenchymal stem cells (pMSCs). Cell Transplant. 2011 20, 1231-1239.
  48. Berglund AK, Schnabel LV. Allogeneic major histocompatibility complex-mismatched equine bone marrow-derived mesenchymal stem cells are targeted for death by cytotoxic anti-major histocompatibility complex antibodies. Equine Vet. J. 2017 49, 539-544.
  49. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential. Stem Cell Res. Ther. 2014 5, 25.
  50. Mundy LN, Ishihara A, Wellman ML, Bertone AL. Evaluation of the ability of a gravitational filtration system to enhance recovery of equine bone marrow elements. Am. J. Vet. Res. 2015 76, 561-569.

Citations

This article has been cited 18 times.
  1. Pezzanite LM, Timkovich AE, Sikes KJ, Chow L, Hendrickson DA, Becker JR, Webster A, Santangelo KS, Dow S. Erythrocyte removal from bone marrow aspirate concentrate improves efficacy as intra-articular cellular therapy in a rodent osteoarthritis model. Ann Transl Med 2023 Jun 30;11(9):311.
    doi: 10.21037/atm-22-4256pubmed: 37404993google scholar: lookup
  2. Kearney CM, Khatab S, van Buul GM, Plomp SGM, Korthagen NM, Labberté MC, Goodrich LR, Kisiday JD, Van Weeren PR, van Osch GJVM, Brama PAJ. Treatment Effects of Intra-Articular Allogenic Mesenchymal Stem Cell Secretome in an Equine Model of Joint Inflammation. Front Vet Sci 2022;9:907616.
    doi: 10.3389/fvets.2022.907616pubmed: 35812845google scholar: lookup
  3. Kamm JL, Riley CB, Parlane NA, Gee EK, McIlwraith CW. Immune response to allogeneic equine mesenchymal stromal cells. Stem Cell Res Ther 2021 Nov 12;12(1):570.
    doi: 10.1186/s13287-021-02624-ypubmed: 34772445google scholar: lookup
  4. Kouchakian MR, Baghban N, Moniri SF, Baghban M, Bakhshalizadeh S, Najafzadeh V, Safaei Z, Izanlou S, Khoradmehr A, Nabipour I, Shirazi R, Tamadon A. The Clinical Trials of Mesenchymal Stromal Cells Therapy. Stem Cells Int 2021;2021:1634782.
    doi: 10.1155/2021/1634782pubmed: 34745268google scholar: lookup
  5. Nino-Fong R, Esparza Gonzalez BP, Rodriguez-Lecompte JC, Montelpare W, McD￮ L. Development of a biologically immortalized equine stem cell line. Can J Vet Res 2021 Oct;85(4):293-301.
    pubmed: 34602734
  6. Rowland AL, Burns ME, Levine GJ, Watts AE. Preparation Technique Affects Recipient Immune Targeting of Autologous Mesenchymal Stem Cells. Front Vet Sci 2021;8:724041.
    doi: 10.3389/fvets.2021.724041pubmed: 34595230google scholar: lookup
  7. Aldrich ED, Cui X, Murphy CA, Lim KS, Hooper GJ, McIlwraith CW, Woodfield TBF. Allogeneic mesenchymal stromal cells for cartilage regeneration: A review of in vitro evaluation, clinical experience, and translational opportunities. Stem Cells Transl Med 2021 Nov;10(11):1500-1515.
    doi: 10.1002/sctm.20-0552pubmed: 34387402google scholar: lookup
  8. Zayed M, Adair S, Dhar M. Effects of Normal Synovial Fluid and Interferon Gamma on Chondrogenic Capability and Immunomodulatory Potential Respectively on Equine Mesenchymal Stem Cells. Int J Mol Sci 2021 Jun 15;22(12).
    doi: 10.3390/ijms22126391pubmed: 34203758google scholar: lookup
  9. Andia I, Maffulli N. Mesenchymal stromal cell products for intra-articular knee injections for conservative management of osteoarthritis. Ther Adv Musculoskelet Dis 2021;13:1759720X21996953.
    doi: 10.1177/1759720X21996953pubmed: 33680097google scholar: lookup
  10. Kamm JL, Riley CB, Parlane N, Gee EK, McIlwraith CW. Interactions Between Allogeneic Mesenchymal Stromal Cells and the Recipient Immune System: A Comparative Review With Relevance to Equine Outcomes. Front Vet Sci 2020;7:617647.
    doi: 10.3389/fvets.2020.617647pubmed: 33521090google scholar: lookup
  11. Popowski K, Lutz H, Hu S, George A, Dinh PU, Cheng K. Exosome therapeutics for lung regenerative medicine. J Extracell Vesicles 2020 Jun 29;9(1):1785161.
    doi: 10.1080/20013078.2020.1785161pubmed: 32944172google scholar: lookup
  12. Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Single and repeated intra-articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow-derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin-1β model of synovitis. Equine Vet J 2020 Jul;52(4):601-612.
    doi: 10.1111/evj.13222pubmed: 31821594google scholar: lookup
  13. Porato M, Antoine N, Waroux O, Piret J, Noël S, Hamaide A. Tracking of autologous adipose-derived mesenchymal stromal/stem cells after intravenous administration: a pilot study in a dog with induced acute bladder injury. Front Vet Sci 2025;12:1644746.
    doi: 10.3389/fvets.2025.1644746pubmed: 41427138google scholar: lookup
  14. Kooy S, Constant J, Cole R, Boone L. Safety study of leucoreduced allogeneic pooled freeze-dried platelet-rich plasma in healthy equine joints. Front Vet Sci 2025;12:1625431.
    doi: 10.3389/fvets.2025.1625431pubmed: 40727274google scholar: lookup
  15. Reis IL, Lopes B, Sousa P, Sousa AC, Rêma A, Caseiro AR, Briote I, Rocha AM, Pereira JP, Mendonça CM, Santos JM, Lamas L, Atayde LM, Alvites RD, Maurício AC. Case report: Equine metacarpophalangeal joint partial and full thickness defects treated with allogenic equine synovial membrane mesenchymal stem/stromal cell combined with umbilical cord mesenchymal stem/stromal cell conditioned medium. Front Vet Sci 2024;11:1403174.
    doi: 10.3389/fvets.2024.1403174pubmed: 38840629google scholar: lookup
  16. Andersen C, Walters M, Bundgaard L, Berg LC, Vonk LA, Lundgren-Åkerlund E, Henriksen BL, Lindegaard C, Skovgaard K, Jacobsen S. Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses. Front Vet Sci 2024;11:1374681.
    doi: 10.3389/fvets.2024.1374681pubmed: 38596460google scholar: lookup
  17. Chang SH, Park CG. Comparing the Benefits and Drawbacks of Stem Cell Therapy Based on the Cell Origin or Manipulation Process: Addressing Immunogenicity. Immune Netw 2023 Dec;23(6):e44.
    doi: 10.4110/in.2023.23.e44pubmed: 38188600google scholar: lookup
  18. Andersen C, Jacobsen S, Uvebrant K, Griffin JF 4th, Vonk LA, Walters M, Berg LC, Lundgren-Åkerlund E, Lindegaard C. Integrin α10β1-Selected Mesenchymal Stem Cells Reduce Pain and Cartilage Degradation and Increase Immunomodulation in an Equine Osteoarthritis Model. Cartilage 2025 Jun;16(2):250-264.
    doi: 10.1177/19476035231209402pubmed: 37990503google scholar: lookup
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