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Veterinary sciences2021; 8(6); 98; doi: 10.3390/vetsci8060098

Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares.

Abstract: So far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF and PGE) in serum of mares with healthy and abnormal uterine status (endometritis). This study was conducted on 80 Arabian mares: young (4-7 years), and old (8-14 years). Mares were divided into 48 sub-fertile (endometritis) and 32 fertile (control) at stud farms. Serum was collected for measuring IL-6, PGF, and PGE, as well as miRNA isolation and qRT-PCR. Concentrations of IL-6, PGE, and PGF were higher in mares with endometritis compared to control. Age of mares had a remarkable effect on IL-6, PGE, and PGF concentrations. Relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was higher in both young and old mares with endometritis. We noticed that eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 revealed higher expression level in old than young mares with endometritis. This is the first study that has revealed the changes in cell free miRNA and serum inflammatory mediators during endometritis, and these findings could be used for a better understanding the pathophysiology mechanisms of endometritis in equine.
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Publication Date: 2021-06-04 PubMed ID: 34199703PubMed Central: PMC8227551DOI: 10.3390/vetsci8060098Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Researchers have explored the potential link between serum microRNA and uterine inflammation in mares particularly focusing on particular types of miRNA and measuring concentrations of inflammatory biomarkers (interleukin 6 and prostaglandins) in healthy mares and those with endometritis.

Research Objective

  • The goal of this study was twofold: firstly, to investigate the expression profile of certain microRNAs (eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205), and secondly, to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF and PGE) in the serum of mares with a healthy uterine status and those with endometritis.

Study Design

  • The research was conducted on 80 Arabian mares divided into two groups based on their age: young (4-7 years) and old (8-14 years).
  • Of these, 48 mares classed as sub-fertile (i.e., suffering from endometritis) and 32 fertile mares served as controls.
  • Serum was collected for measuring IL-6, PGF, and PGE, as well as for miRNA isolation and analysis using qRT-PCR, a method for quantifying RNA levels.

Findings

  • Results indicated that the concentrations of IL-6, PGE, and PGF were higher in mares with endometritis compared to the control group. Additionally, the age of the mares had a significant impact on IL-6, PGE, and PGF concentrations.
  • The relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was found to be higher in both young and old mares with endometritis.
  • Of note, eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 displayed higher expression levels in older mares with endometritis compared to younger ones.

Significance of the Study

  • This is the first study highlighting the changes in cell-free miRNA and serum inflammatory mediators during endometritis in mares. Such findings potentially pave the way to a better understanding of the pathophysiological mechanisms of endometritis in equine species.

Cite This Article

APA
Ibrahim S, Hedia M, Taqi MO, Derbala MK, Mahmoud KGM, Ahmed Y, Ismail S, El-Belely M. (2021). Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares. Vet Sci, 8(6), 98. https://doi.org/10.3390/vetsci8060098

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 8
Issue: 6
PII: 98

Researcher Affiliations

Ibrahim, Sally
  • Department of Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Giza 12622, Egypt.
Hedia, Mohamed
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Taqi, Mohamed O
  • Central Laboratory for Agricultural Climate, Agricultural Research Centre, Ministry of Agriculture and Land Reclamation, Dokki, Giza 12311, Egypt.
Derbala, Mohamed K
  • Diagnostic Imaging and Endoscopy Unit, Animal Reproduction Research Institute, Giza 12556, Egypt.
Mahmoud, Karima Gh M
  • Department of Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Giza 12622, Egypt.
Ahmed, Youssef
  • Department of Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Giza 12622, Egypt.
Ismail, Sayed
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
El-Belely, Mohamed
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Grant Funding

  • 33342 / Science and Technology Development Fund (STDF), Egypt

Conflict of Interest Statement

The authors declare no conflict of interest.

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