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Home / Equine Research Bank / Reprod Domest Anim / Alternatives in Donkey semen cryopreservation: Mare vs. Jenn...
Reproduction in domestic animals = Zuchthygiene2019; 54 Suppl 4; 94-97; doi: 10.1111/rda.13516

Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum.

Abstract: The aim of this study was to test and compare two new components in extenders for freezing donkey semen: mare colostrum and jenny colostrum. Colostrum was obtained from four mares and four jennies right after the foal's birth. Ejaculates were collected from five fertile donkeys. Sperm samples were pooled, diluted and cryopreserved in three different experimental extender groups: lactose supplemented with egg yolk extender (20%) as the control group, lactose supplemented with jenny colostrum extender (20%), and lactose supplemented with mare colostrum extender (20%). After thawing, we evaluated the sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional by HOS test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. The results demonstrated that lactose-jenny colostrum extender displayed significantly higher values (p < .05) in nearly all parameters evaluated - Total Motility, Viability, HOS test, VCL, VSL, VAP, LIN, STR and WOB -, compared with mare colostrum and egg yolk extenders after thawing. In conclusion, the extender containing jenny colostrum used for donkey semen cryopreservation improved the donkey sperm quality after the freezing-thawing process.
© 2019 Blackwell Verlag GmbH.
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Publication Date: 2019-10-19 PubMed ID: 31625242DOI: 10.1111/rda.13516Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research experiment attempts to improve sperm quality following cryopreservation in donkeys, testing the use of colostrum obtained from mares and jennies as new components in extenders. The findings suggest that extenders containing jenny colostrum were significantly more successful in maintaining sperm quality post-freezing.

Overview of Experimental Design

  • The research involved collection of colostrum from four mares and four jennies and semen from five fertile donkeys. Colostrum is the initial form of milk produced immediately after birth that contains antibodies to boost the newborn’s immunity.
  • The researchers tested three variations of extenders used during freezing: lactose-egg yolk based extender as a control, lactose-jenny colostrum-based and lactose-mare colostrum-based extenders.

Sperm Sample Preparation and Preservation

  • Sperm samples from donkeys were combined, diluted, and cryopreserved in the different extender groups. Cryopreservation is the process by which cells are frozen to preserve them for later use.
  • The semen samples were evaluated post-thawing on various parameters to ascertain the effectiveness of the different extenders.

Evaluation and Results

  • Post-thawing analysis involved evaluation of sperm motility using computer-assisted analysis, viability determined by SYBR-14 and propidium iodide (PI), membrane functionality tested by HOS test and acrosome integrity checked using isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI.
  • Almost all parameters evaluated—total motility, viability, HOS test score, VCL, VSL, VAP, LIN, STR and WOB—displayed significantly better results in the lactose-jenny colostrum extender.

Conclusion

  • The study concludes that jenny colostrum extenders used for donkey semen cryopreservation considerably enhance the quality of donkey sperm after the freezing-thawing process.

Cite This Article

APA
Álvarez C, González N, Luño V, Martínez F, Gil L. (2019). Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum. Reprod Domest Anim, 54 Suppl 4, 94-97. https://doi.org/10.1111/rda.13516

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 54 Suppl 4
Pages: 94-97

Researcher Affiliations

Álvarez, Cristina
  • Horse Breeding Military Center in Zaragoza, Zaragoza, Spain.
González, Noelia
  • Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.
Luño, Victoria
  • Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.
Martínez, Felisa
  • Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.
Gil, Lydia
  • Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.

MeSH Terms

  • Animals
  • Colostrum
  • Cryopreservation / methods
  • Cryopreservation / veterinary
  • Cryoprotective Agents / pharmacology
  • Equidae
  • Female
  • Horses
  • Male
  • Semen Analysis / veterinary
  • Semen Preservation / methods
  • Semen Preservation / veterinary
  • Sperm Motility / drug effects

References

This article includes 19 references
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Citations

This article has been cited 1 times.
  1. Catalán J, Yánez-Ortiz I, Tvarijonaviciute A, González-Arostegui LG, Rubio CP, Yeste M, Miró J, Barranco I. Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance.. Antioxidants (Basel) 2022 Feb 18;11(2).
    doi: 10.3390/antiox11020417pubmed: 35204299google scholar: lookup
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