Amino acid sequence of the beta-subunit of the follicle-stimulating hormone from equine pituitary glands.
Abstract: A tentative amino acid sequence of the beta-subunit of equine follicle-stimulating hormone (FSH) was derived from the sequences of tryptic, thermolytic as well as peptic, subtilisin, and chymotryptic peptides. Equine FSH-beta is analogous to human FSH-beta except six amino acid substitutions at positions 12, 16, 21, 62, 108, and 114. The amino acid sequence suggests that the hormone-specific beta-subunits of FSH are similar at the interspecies level, whereas the amino acid sequences of the hormone nonspecific alpha-subunits show variations.
Publication Date: 1978-08-10 PubMed ID: 670202
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article focuses on studying the amino acid sequence of the beta-subunit of the follicle-stimulating hormone (FSH) from equine pituitary glands, and comparing it with the human FSH-beta.
Amino Acid Sequence of FSH-beta
- The main part of the research focused on deriving the tentative amino acid sequence of the equine follicle-stimulating hormone (FSH) beta-subunit. This was achieved by using multiple procedures, such as tryptic, thermolytic as well as peptic, subtilisin, and chymotryptic peptides sequences.
- The role of FSH is crucial as it is responsible for the growth and development of eggs in the ovaries and the production of sperm in men. This hormone’s structure includes two sub-units – namely, the alpha and beta sub-units.
Comparison with Human FSH-beta
- The derived amino acid sequence of equine FSH-beta was compared to that of human FSH-beta. The study found that equine FSH-beta is similar to human FSH-beta, except there were six amino acid substitutions at positions 12, 16, 21, 62, 108, and 114.
Similarities and Variations Within and Across Species
- The research study concludes that hormone-specific beta-subunits of FSH are similar at the interspecies level implying a possible universal structure and function across different mammalian species.
- However, there were observed variations in the amino acid sequences of the hormone non-specific alpha-subunits among different species; suggesting there could be role variations or diverse functionalities offered by the alpha sub-unit.
Cite This Article
APA
Fujiki Y, Rathnam P, Saxena BB.
(1978).
Amino acid sequence of the beta-subunit of the follicle-stimulating hormone from equine pituitary glands.
J Biol Chem, 253(15), 5363-5368.
Publication
Researcher Affiliations
MeSH Terms
- Amino Acid Sequence
- Animals
- Follicle Stimulating Hormone / isolation & purification
- Horses
- Humans
- Macromolecular Substances
- Peptide Fragments / analysis
- Pituitary Gland
- Species Specificity
Citations
This article has been cited 7 times.- Yoo SK, Mehdi SF, Pusapati S, Mathur N, Anipindi M, Lunenfeld B, Lowell B, Yang H, Metz CN, Khan SA, Leroith D, Roth J. Human Chorionic Gonadotropin and Related Peptides: Candidate Anti-Inflammatory Therapy in Early Stages of Sepsis. Front Immunol 2021;12:714177.
- Sairam MR, Seidah NG, Chrétien M. Primary structure of the ovine pituitary follitropin beta-subunit. Biochem J 1981 Sep 1;197(3):541-52.
- Sairam MR. Primary structure of the ovine pituitary follitropin alpha-subunit. Biochem J 1981 Sep 1;197(3):535-9.
- Shome B, Parlow AF, Liu WK, Nahm HS, Wen T, Ward DN. A reevaluation of the amino acid sequence of human follitropin beta-subunit. J Protein Chem 1988 Aug;7(4):325-39.
- Esch FS, Mason AJ, Cooksey K, Mercado M, Shimasaki S. Cloning and DNA sequence analysis of the cDNA for the precursor of the beta chain of bovine follicle stimulating hormone. Proc Natl Acad Sci U S A 1986 Sep;83(17):6618-21.
- Sugino H, Takio K, Ward DN. Reevaluation of the amino acid sequence of porcine follitropin. J Protein Chem 1989 Apr;8(2):197-219.
- Stockell Hartree A, Renwick AG. Molecular structures of glycoprotein hormones and functions of their carbohydrate components. Biochem J 1992 Nov 1;287 ( Pt 3)(Pt 3):665-79.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
