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[An analysis of reproducibility in the determination of the activity of selected enzymes in the blood serum of horses].
Abstract: Single biochemical analyses can be used for the diagnosis of animal diseases only with the knowledge of the effects that may distort the single result. The study of the repeatability of analyses is described in the four basic enzymes (AST, ALP, GMT, LD), which are most frequently used for diagnosis. The experiment was conducted in a group of ten Kladrub mares. Six blood samples were taken from each of the mares within ten days. The measured values were subjected to statistical processing and repeatability coefficients (r op) were calculated. All the r op values were high (ALP--0.96, LD--0.93, GMT--0.90, AST--0.60). The obtained repeatability coefficients are discussed for each enzyme, including their sources and diagnostic function.
Publication Date: 1984-03-01 PubMed ID: 6426128 The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- English Abstract
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the reproducibility of biochemical analyses used in diagnosis of diseases in horses focusing on four frequently used enzymes. To accomplish this, ten mares were sampled and the results underwent statistical evaluation to determine the repeatability coefficients of the enzyme measurements.
Research Overview
The paper aims to provide a comprehensive analysis on how reproducible biochemical analyses are when determining the activity of selected enzymes in horse’s blood serum. These enzymes include AST (Aspartate Aminotransferase), ALP (Alkaline Phosphatase), GMT (Gamma-Glutamyl Transferase), and LD (Lactate Dehydrogenase), which are often used for diagnosing illnesses in horses.
Methodology
- Ten mares from the Kladrub breed were selected as the sample size for this experiment.
- Six blood samples were taken from each mare over a ten-day period.
- The enzyme values from these samples were then subjected to statistical analysis.
Results
The statistical processing led to the calculation of repeatability coefficients (r op values) which reflect the reproducibility of each tested enzyme. This means the higher the value, the more consistent the enzyme measurements were across the different samples, pointing towards a greater degree of repeatability.
- ALP had the highest coefficient with 0.96.
- LD followed closely with a coefficient of 0.93.
- GMT displayed a coefficient of 0.90.
- AST had the lowest one, which was 0.6.
Discussion
After the results were obtained, the paper goes ahead to discuss the calculated coefficients for each enzyme. This would include:
- The significance of each enzyme in relation to its reproducibility.
- Where these enzymes are sourced from and their diagnostic function when conducting biochemical analyses in horses.
With the obtained r op values, the study opens up a conversation on the validity of testing these four enzymes consistently for diagnostic purposes.
Cite This Article
APA
Kovár J.
(1984).
[An analysis of reproducibility in the determination of the activity of selected enzymes in the blood serum of horses].
Vet Med (Praha), 29(3), 163-170.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Clinical Enzyme Tests / veterinary
- Female
- Horses / blood
Citations
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