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In vitro cellular & developmental biology : journal of the Tissue Culture Association1992; 28A(1); 11-16; doi: 10.1007/BF02631074

An inhibitor of tumor cell growth from normal horse serum.

Abstract: During our studies of cytostatic cytokines in the mixed leukocyte culture, we found that horse serum in the medium control contained a tumor cell growth-inhibitory factor. The fraction isolated by molecular sieving and ion exchange chromatography inhibited the growth and DNA synthesis of the primary culture and passaged cell line of the canine transmissible venereal sarcoma, murine T (L5178Y) and B (P3-X63-Ag8.653) lymphoid tumor cells, murine mammary tumor cells (RIII), bovine lymphoid tumor cells (BL3), and the nontransformed cell line of baby hamster kidney cells. Nontransformed cell lines such as African green monkey kidney (Vero) and Madin-Darby bovine kidney cells and normal mammary cells of the dog and goat were inhibited only at high concentrations. The active component is a protein with an Rf value of 0.09 upon electrophoresis in native 7.5% polyacrylamide gels. The eluate from the native gel could be further separated into three polypeptides with molecular weights of approximately 67, 65, and 55 kDa under reduced conditions in sodium dodecyl sulfate-polyacrylamide gels.
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Publication Date: 1992-01-01 PubMed ID: 1730565DOI: 10.1007/BF02631074Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This article discusses the discovery of a tumor cell growth-inhibiting factor in horse serum, which hampers the growth and DNA synthesis of various types of cancer cells in both animals and humans when applied in certain concentrations.

Discovery of Tumor Cell Inhibitor

The study began with a focus on cytostatic cytokines in the mixed leukocyte culture. The researchers observed that the medium control horse serum used in the experiment contained an agent that demonstrated the ability to inhibit tumor cell growth.

Laboratory Testing

  • The growth-inhibitory factor was isolated through molecular sieving and ion exchange chromatography.
  • The active component was applied on several primary and passaged cell lines from different types of cancer found in animals, including dogs, mice, cows, and hamsters. Notably, these included canine transmissible venereal sarcoma, murine T (L5178Y) and B (P3-X63-Ag8.653) lymphoid tumor cells, murine mammary tumor cells (RIII), bovine lymphoid tumor cells (BL3), and non-transformed cell lines of baby hamster kidney cells.
  • The results showed that the growth and DNA synthesis of these cancer cells were inhibited.

Impact on Non-transformed Cells

  • Non-transformed cell lines, such as African green monkey kidney (Vero) and Madin-Darby bovine kidney cells and normal mammary cells of the dog and goat, were also tested.
  • The growth of these cells was only inhibited at high concentrations, indicating that the cancer-inhibiting protein could have less of an impact on normal, non-cancerous cells, reducing potential side effects when used in cancer therapy.

Protein Analysis

  • The growth-inhibitory factor was found to be a protein with an Rf value of 0.09 when performed electrophoresis in native 7.5% polyacrylamide gels.
  • Upon further analysis, it was found that the eluate from the native gel could be split into three polypeptides with molecular weights of approximately 67, 65, and 55 kDa under reduced conditions in sodium dodecyl sulfate-polyacrylamide gels.

Cite This Article

APA
Ericson KK, Yang TJ. (1992). An inhibitor of tumor cell growth from normal horse serum. In Vitro Cell Dev Biol, 28A(1), 11-16. https://doi.org/10.1007/BF02631074

Publication

In vitro cellular & developmental biology : journal of the Tissue Culture Association
ISSN: 0883-8364
NlmUniqueID: 8506951
Country: United States
Language: English
Volume: 28A
Issue: 1
Pages: 11-16

Researcher Affiliations

Ericson, K K
  • Department of Pathobiology, University of Connecticut, Storrs 06269.
Yang, T J

    MeSH Terms

    • Animals
    • Antineoplastic Agents / isolation & purification
    • Blood
    • Blood Proteins / isolation & purification
    • Blood Proteins / pharmacology
    • Cell Division / drug effects
    • Cell Line
    • Cell Line, Transformed
    • Chromatography, Gel
    • Chromatography, Ion Exchange
    • DNA Replication / drug effects
    • Dogs
    • Electrophoresis, Polyacrylamide Gel
    • Horses
    • Mice

    References

    This article includes 10 references
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    Citations

    This article has been cited 1 times.
    1. Ichinose I, Nakano S, Esaki T, Koga T, Mitsugi K, Yamada H, Niho Y. Growth modulation of human tumor cells by a growth-inhibiting activity derived from tumorigenic V79 Chinese hamster cells. In Vitro Cell Dev Biol Anim 1993 Apr;29A(4):332-8.
      doi: 10.1007/BF02633962pubmed: 8320183google scholar: lookup
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