Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in vitro.
Abstract: The purpose of this study was to investigate the response of suspensory ligament fibroblasts (SLF) to in vitro stimulation using acellular bone marrow (ABM), platelet rich plasma (in vitro PRP), and serum as potential treatment modalities for suspensory desmitis. Blood, bone marrow, and suspensory ligaments were collected from five horses. SLF were harvested, grown until confluent, and stimulated with various concentrations of ABM, PRP, equine serum, foetal bovine serum, and medium (control). The responses to the treatments were assessed using a combination of radio-labeling for total protein synthesis and an ELISA for quantification of Cartilage Oligomeric Matrix Protein (COMP) production. Addition of all of the samples resulted in significant increases in COMP and total protein synthesis over controls (P<0.001). ABM caused the greatest increase in both COMP and total protein synthesis by the SLF. Equine ABM, PRP, and serum contain anabolic factors that promote matrix synthesis by SLF in vitro, with ABM having the greatest effect. Application of bone marrow to injured ligaments may enhance healing by providing anabolic factors, other than or in addition to mesenchymal stem cells, which stimulate matrix production.
Publication Date: 2006-04-06 PubMed ID: 16594543
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research studied the potential effects of acellular bone marrow (ABM), platelet rich plasma (PRP), and serum on horse suspensory ligament fibroblasts, finding that these substances significantly increased levels of Cartilage Oligomeric Matrix Protein and protein synthesis, especially ABM, indicating possible benefits in healing injured ligaments in horses.
Research Objective
- The aim of the research was to analyze the impact of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in a controlled lab environment.
- The goal was to identify these substances’ potential in treating suspensory desmitis, a common condition causing lameness in horses.
Methodology
- Blood, bone marrow, and suspensory ligaments were collected from five horses for the study.
- Suspensory ligament fibroblasts (SLF) were harvested and grown until they reached confluence – the stage of growth where cells fill up all available space.
- The matured cells were then stimulated with different concentrations of the substances – acellular bone marrow (ABM), platelet rich plasma (PRP), equine serum, fetal bovine serum and a control medium.
- Response to these substances was measured via protein synthesis radio-labeling and an enzyme-linked immunosorbent assay (ELISA) for quantifying cartilage oligomeric matrix protein (COMP) production.
Findings
- All samples resulted in significant increases in both COMP production and total protein synthesis over controls.
- It was observed that ABM led to the most substantial increase in both COMP and total protein synthesis by the SLF.
Implications and Conclusions
- The substantial increases in COMP and protein synthesis indicate that ABM, PRP, and serum contain anabolic factors that promote matrix synthesis by SLF in a lab setting.
- ABM was found to have the most substantial effect among the substances tested.
- The research suggests that applying bone marrow to injured ligaments could improve healing by providing factors that, either on their own or in conjunction with mesenchymal stem cells, stimulate matrix production.
- This insight could be vital for aiding in the treatment of suspensory desmitis in horses, a condition that currently lacks comprehensive and uniformly successful treatment methods.
Cite This Article
APA
Smith JJ, Ross MW, Smith RK.
(2006).
Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in vitro.
Vet Comp Orthop Traumatol, 19(1), 43-47.
Publication
Researcher Affiliations
- Department of Veterinary Clinical Studies, University of Pennsylvania, Kennett Square 19348, USA. smithjj@vet.upenn.edu
MeSH Terms
- Animals
- Blood Platelets / metabolism
- Blood Platelets / physiology
- Bone Marrow / metabolism
- Bone Marrow / physiology
- Cells, Cultured
- Culture Media
- Extracellular Matrix Proteins / biosynthesis
- Female
- Fibroblasts / metabolism
- Glycoproteins / biosynthesis
- Horse Diseases / metabolism
- Horses
- Inflammation / metabolism
- Inflammation / veterinary
- Ligaments / metabolism
- Male
- Matrilin Proteins
- Serum / metabolism
- Serum / physiology
Citations
This article has been cited 19 times.- Chalidis B, Givissis P, Papadopoulos P, Pitsilos C. Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review.. Int J Mol Sci 2023 Feb 1;24(3).
- DeMello V, Chen G, Wakshlag J, Mason D. Evaluation of Platelet and Leukocyte Counts in Canine Platelet-Rich Plasma Obtained After Successive Blood Collections From the Same Patient and the Effects of Freezing on the Concentration of Growth Factors Present in It.. Front Vet Sci 2022;9:838481.
- Kunze KN, Pakanati JJ, Vadhera AS, Polce EM, Williams BT, Parvaresh KC, Chahla J. The Efficacy of Platelet-Rich Plasma for Ligament Injuries: A Systematic Review of Basic Science Literature With Protocol Quality Assessment.. Orthop J Sports Med 2022 Feb;10(2):23259671211066504.
- Maleas G, Mageed M. Effectiveness of Platelet-Rich Plasma and Bone Marrow Aspirate Concentrate as Treatments for Chronic Hindlimb Proximal Suspensory Desmopathy.. Front Vet Sci 2021;8:678453.
- Montano C, Auletta L, Greco A, Costanza D, Coluccia P, Del Prete C, Meomartino L, Pasolini MP. The Use of Platelet-Rich Plasma for Treatment of Tenodesmic Lesions in Horses: A Systematic Review and Meta-Analysis of Clinical and Experimental Data.. Animals (Basel) 2021 Mar 12;11(3).
- Osborn ML, Cornille JL, Blas-Machado U, Uhl EW. The equine navicular apparatus as a premier enthesis organ: Functional implications.. Vet Surg 2021 May;50(4):713-728.
- Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
- Ferrari JT, Schwartz P. Prospective Evaluation of Feline Sourced Platelet-Rich Plasma Using Centrifuge-Based Systems.. Front Vet Sci 2020;7:322.
- Zheng H, Huang W, He B, Tan H, Lin P, Zha Z. Positive effects of platelet-rich plasma (PRP) and a Sanguisorba officinalis polysaccharide on the proliferation and differentiation of anterior cruciate ligament (ACL) fibroblasts in vitro.. Pharm Biol 2020 Dec;58(1):297-305.
- Kornicka-Garbowska K, Pędziwiatr R, Woźniak P, Kucharczyk K, Marycz K. Microvesicles isolated from 5-azacytidine-and-resveratrol-treated mesenchymal stem cells for the treatment of suspensory ligament injury in horse-a case report.. Stem Cell Res Ther 2019 Dec 18;10(1):394.
- Deal JB, Smith E, Heard W, O'Brien MJ, Savoie FH 3rd. Platelet-Rich Plasma for Primary Treatment of Partial Ulnar Collateral Ligament Tears: MRI Correlation With Results.. Orthop J Sports Med 2017 Nov;5(11):2325967117738238.
- Canapp SO Jr, Leasure CS, Cox C, Ibrahim V, Carr BJ. Partial Cranial Cruciate Ligament Tears Treated with Stem Cell and Platelet-Rich Plasma Combination Therapy in 36 Dogs: A Retrospective Study.. Front Vet Sci 2016;3:112.
- Geburek F, Gaus M, van Schie HT, Rohn K, Stadler PM. Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial.. BMC Vet Res 2016 Sep 7;12(1):191.
- Carr BJ, Canapp SO Jr, Mason DR, Cox C, Hess T. Canine Platelet-Rich Plasma Systems: A Prospective Analysis.. Front Vet Sci 2015;2:73.
- Metcalf KB, Mandelbaum BR, McIlwraith CW. Application of Platelet-Rich Plasma to Disorders of the Knee Joint.. Cartilage 2013 Oct;4(4):295-312.
- Brossi PM, Moreira JJ, Machado TS, Baccarin RY. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions.. BMC Vet Res 2015 Apr 22;11:98.
- Smith RK, Werling NJ, Dakin SG, Alam R, Goodship AE, Dudhia J. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.. PLoS One 2013;8(9):e75697.
- Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine.. Can Vet J 2009 Feb;50(2):155-65.
- Lyras D, Kazakos K, Verettas D, Polychronidis A, Simopoulos C, Botaitis S, Agrogiannis G, Kokka A, Patsouris E. Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect.. Int Orthop 2010 Feb;34(1):143-8.
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