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Home / Equine Research Bank / J Biomed Biotechnol / Analysis of horse myostatin gene and identification of singl...
Journal of biomedicine & biotechnology2010; 2010; 542945; doi: 10.1155/2010/542945

Analysis of horse myostatin gene and identification of single nucleotide polymorphisms in breeds of different morphological types.

Abstract: Myostatin (MSTN) is a negative modulator of muscle mass. We characterized the horse (Equus caballus) MSTN gene and identified and analysed single nucleotide polymorphisms (SNPs) in breeds of different morphological types. Sequencing of coding, untranslated, intronic, and regulatory regions of MSTN gene in 12 horses from 10 breeds revealed seven SNPs: two in the promoter, four in intron 1, and one in intron 2. The SNPs of the promoter (GQ183900:g.26T>C and GQ183900:g.156T>C, the latter located within a conserved TATA-box like motif) were screened in 396 horses from 16 breeds. The g.26C and the g.156C alleles presented higher frequency in heavy (brachymorphic type) than in light breeds (dolichomorphic type such as Italian Trotter breed). The significant difference of allele frequencies for the SNPs at the promoter and analysis of molecular variance (AMOVA) on haplotypes indicates that these polymorphisms could be associated with variability of morphology traits in horse breeds.
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Publication Date: 2010-07-14 PubMed ID: 20706663PubMed Central: PMC2913906DOI: 10.1155/2010/542945Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research studied the myostatin gene in horses and identified variations in single units, or nucleotides, of DNA in horse breeds of different body builds.

Introduction

  • The focus of this research was the myostatin gene (MSTN) in horses (Equus caballus). Myostatin is known as a detractor of muscle mass; in other words, it modulates the development and growth of muscles.
  • The researchers aimed to identify and analyze single nucleotide variations (SNPs) in horse breeds of different physical structures. Single nucleotide polymorphism refers to a variation at a single place in a DNA sequence among individuals. Such variations can have significant impacts on individual traits and susceptibility to certain diseases.

Research Methodology

  • The team sequenced the coding, untranslated, intronic, and regulatory regions of the MSTN gene in 12 horses from 10 different breeds. This was done to study the structure of the gene and to locate the SNPs.
  • The process revealed seven SNPs: two in the promoter area (which initiates the transcription of a gene), four in intron 1 (a non-coding sector within a gene), and one in intron 2.

Findings

  • The researchers screened the two SNPs found in the promoter region in 396 horses from 16 breeds. One of the promoter SNPs was located within a conserved TATA-box like motif, which is a critical DNA sequence in the transcription process.
  • They discovered that the g.26C and the g.156C alleles (alternative forms of a gene) were more frequent in heavy breeds (considered brachymorphic) than in light breeds (termed dolichomorphic), such as the Italian Trotter breed.
  • The significant difference in allele frequencies for the SNPs at the promoter and the analysis of molecular variance (AMOVA) on haplotypes (specific group of genes within an organism that is inherited together from a single parent) suggest that these variations could be associated with the variability of morphology traits in different horse breeds.

Conclusion

  • The results can shed light on the genetic basis of physiological differences among horse breeds. This gene-level understanding could potentially guide future breeding or conservation programs, aiming for specific morphological traits in horses.

Cite This Article

APA
Dall'Olio S, Fontanesi L, Nanni Costa L, Tassinari M, Minieri L, Falaschini A. (2010). Analysis of horse myostatin gene and identification of single nucleotide polymorphisms in breeds of different morphological types. J Biomed Biotechnol, 2010, 542945. https://doi.org/10.1155/2010/542945

Publication

Journal of biomedicine & biotechnology
ISSN: 1110-7251
NlmUniqueID: 101135740
Country: United States
Language: English
Volume: 2010
PII: 542945

Researcher Affiliations

Dall'Olio, Stefania
  • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, 42123 Reggio Emilia, Italy.
Fontanesi, Luca
    Nanni Costa, Leonardo
      Tassinari, Marco
        Minieri, Laura
          Falaschini, Adalberto

            MeSH Terms

            • Analysis of Variance
            • Animals
            • Base Sequence
            • Cattle
            • Haplotypes
            • Horses / anatomy & histology
            • Horses / genetics
            • Humans
            • Mice
            • Molecular Sequence Data
            • Myostatin / genetics
            • Phenotype
            • Polymorphism, Single Nucleotide
            • Sequence Alignment
            • Somatotypes
            • Species Specificity

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            Citations

            This article has been cited 20 times.
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