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Journal of clinical microbiology1991; 29(5); 889-893; doi: 10.1128/jcm.29.5.889-893.1991

Analysis of serotypes and electropherotypes of equine rotaviruses isolated in the United States.

Abstract: Equine group A rotaviruses isolated over a 10-year period in New York State, New Jersey, Kentucky, and Texas were compared serotypically and electropherotypically. All isolates were determined to be serotype 3 by reaction with hyperimmune antiserum to the serotype 3 H-2 strain of equine rotavirus. All displayed RNA electrophoretic migration patterns related to that of the H-2 strain but distinct from that of serotype 5 strain H-1. A serologic survey of 184 mares in Kentucky, which was done to determine the incidence of H-1 and H-2 infections, showed geometric mean serum neutralizing titers to the H-2 strain of equine rotavirus to be significantly higher than those to the H-1 strain. These data suggest that the serotype 3 H-2 strain is the dominant equine rotavirus in Kentucky and perhaps elsewhere in the United States. We were unable to produce confirmational evidence that the H-1 strain occurs as a natural infection in the United States.
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Publication Date: 1991-05-01 PubMed ID: 1647407PubMed Central: PMC269902DOI: 10.1128/jcm.29.5.889-893.1991Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses an analysis carried out on samples of equine rotaviruses collected in the US over a span of 10 years. The main finding of the study is that the serotype 3 H-2 strain is likely the dominant strain of equine rotavirus in the United States.

Analysis of Equine Rotaviruses

  • The study focuses on equine group A rotaviruses, which were isolated over a decade from various states in the United States – New York, New Jersey, Kentucky, and Texas.
  • The rotaviruses were studied and compared using their serotypes – classification based on their reaction to hyperimmune antiserum, and electropherotypes – classification based on their RNA migration patterns.

Dominance of Serotype 3

  • Findings from the study concluded all isolates to be serotype 3, struck by the reaction to the hyperimmune antiserum to the serotype 3 H-2 strain of equine rotavirus.
  • The RNA electrophoretic migration patterns of these isolates displayed resemblance to that of the H-2 strain but deviated significantly from the serotype 5 strain H-1.
  • Serotype 3’s dominance is reinforced by an associated serologic survey conducted amongst 184 mares in Kentucky. This survey aimed to gauge the incidence of H-1 and H-2 infections by comparing geometric mean serum neutralizing titers between both strains.
  • The results indicated higher titers for the H-2 strain in comparison to the H-1 strain, making its occurrence more prevalent and suggesting it to be the dominant strain in the Kentucky region and potentially other regions in the United States.

Lack of Evidence for H-1 Strain

  • The research was not able to provide strong evidence for the presence or the natural infection of the H-1 strain in the United States.
  • While the study did not entirely rule out the strain’s existence, the lack of confirmational evidence limits the study’s conclusions regarding the prevalence of this serotype.

Cite This Article

APA
Hardy ME, Woode GN, Xu ZC, Williams JD, Conner ME, Dwyer RM, Powell DG. (1991). Analysis of serotypes and electropherotypes of equine rotaviruses isolated in the United States. J Clin Microbiol, 29(5), 889-893. https://doi.org/10.1128/jcm.29.5.889-893.1991

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 29
Issue: 5
Pages: 889-893

Researcher Affiliations

Hardy, M E
  • Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
Woode, G N
    Xu, Z C
      Williams, J D
        Conner, M E
          Dwyer, R M
            Powell, D G

              MeSH Terms

              • Animals
              • Electrophoresis, Polyacrylamide Gel
              • Female
              • Horse Diseases / microbiology
              • Horses / microbiology
              • Pregnancy
              • RNA, Viral / genetics
              • RNA, Viral / isolation & purification
              • Rotavirus / classification
              • Rotavirus / genetics
              • Rotavirus / isolation & purification
              • Rotavirus Infections / microbiology
              • Rotavirus Infections / veterinary
              • Serotyping
              • United States

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              Citations

              This article has been cited 15 times.
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