Survey of equine rotaviruses shows conservation of one P genotype in background of two G genotypes.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This study analyzed the different types of rotavirus found in horses, discovering that the virus population predominantly has one P genotype and two G genotypes, providing insight into how to combat this common cause of diarrhea in young horses.
Research Overview
The research focused on the genotyping of group A equine rotaviruses, conducting hybridization assays using DIG-labelled ssRNA probes made from variable regions of VP4 and VP7 genes. The researchers examined both cell culture-adapted rotaviruses and fecal samples obtained from foals, both healthy and suffering from diarrhea.
Results
- The probes confirmed the known P and G serotypes, both classes of antigens that allow for the classification of rotaviruses, in sixteen cell-cultured strains.
- Of 121 rotavirus-positive samples, 83 reacted when tested with their specific P and G genotype probes.
- From these, 71 were found to be of the G3 P12 genotypes, while 11 were of the G14 P12 genotypes.
- No samples reacted with the H1 or L338 P and G genotype probes.
Significance of Findings
The findings suggest that the equine rotavirus population is conservative in nature, predominantly containing one P genotype (P12) and two G genotypes (G3 and G14). These insights are valuable in understanding the spread of the virus and how best to combat it.
Unique Isolate and Untypeable Isolates
- One unique isolate was found that did not react with any of the equine probes. Upon further investigation, it was found to belong to the G8 and P1 genotypes. This is only the fourth unique equine isolate discovered, following H1, L338, and R-22.
- Also, thirty-eight field isolates were found untypable as they had no detectable dsRNA, possibly due to degradation during storage for 1 to 3 years.
This research provides valuable insights into equine rotavirus genotypes and their distribution, which can be influential in further research and development of treatment and preventive measures.
Cite This Article
Publication
Researcher Affiliations
- Moredun Research Institute, Edinburgh, U.K.
MeSH Terms
- Animals
- Antigens, Viral
- Blotting, Northern / methods
- Blotting, Southern / methods
- Capsid / genetics
- Capsid Proteins
- Cell Line
- Genotype
- Horse Diseases
- Horses
- RNA Probes
- Rotavirus / classification
- Rotavirus / genetics
- Rotavirus / isolation & purification
- Rotavirus Infections / veterinary
- Rotavirus Infections / virology
- Sensitivity and Specificity
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