English
French
Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H NMR).
Abstract: Follicular development and ovulatory processes in mammals involve local biochemical changes as a result of substantial modifications in cellular metabolism, the most well known of which is steroid variation. In the present study, the intrafollicular variation of several other components was studied using proton nuclear magnetic resonance ((1)H NMR). This approach made it possible to demonstrate that the intrafollicular biochemical content changes during follicular growth and maturation. Follicular fluid was aspirated by ovarian puncture of the dominant follicle at various physiological stages of its development: early dominant, late dominant and preovulatory. Serum samples were collected during each puncture session. (1)H NMR was used to evaluate intrafollicular and circulating glycoconjugates (sugar chains and N-acetyl groups), lipoproteins (CH(3) and CH(2) groups), glucose metabolites (trimethylamines, acetate and lactate), amino acids (glutamine/glutamate and alanine), creatine/creatinine and polyamines. Follicular fluids were assayed by radioimmunoassay for oestradiol and progesterone contents. The intrafollicular contents of alanine and lipoproteins (CH(3) groups) decreased in the dominant follicle during growth, whereas concentrations of progesterone and oestradiol increased significantly. After injection of gonadotrophin to induce ovulation, follicular maturation was characterized by a decrease in glycoconjugates (sugar chains), trimethylamines and acetate, a decrease in oestradiol concentration, and a further increase in CH(3) groups of lipoproteins and progesterone. The results from the present study showed a clear correlation between the intrafollicular content of alanine and that of oestradiol. A correlation between progesterone and glycoconjugates (sugar chains) was also observed. Therefore, (1)H NMR was shown to be effective for studying specific changes in the biochemical composition of the follicular fluid that occur during follicular development. For the first time, the variation of several compounds (glycoconjugates, lipoproteins, glucose metabolites, amino acids and polyamines) in relation to growth and maturation was demonstrated. Some of these changes could be of crucial importance for follicular maturation and ovulation as well as for oocyte maturation and further fertilization.
Publication Date: 2002-07-27 PubMed ID: 12141937DOI: 10.1530/rep.0.1240241Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study investigates the changes in the biochemical composition of the follicular fluid during follicle development and maturation in mares using proton nuclear magnetic resonance (1H NMR). It shows for the first time how multiple compounds vary in relation to this growth and maturation, some of which may be critical for maturation and ovulation of the follicle, and ultimately, fertilization of the oocyte.
Methodology
- The researchers focused on follicle development and ovulatory processes in mammals which involve local biochemical changes due to cellular metabolism modifications. The variations of several components were studied using (1)H NMR.
- Follicular fluid was extracted through ovarian puncture at various physiological stages: early dominant, late dominant and preovulatory. At the same time, serum samples were collected.
- The (1)H NMR was subsequently used to assess the intrafollicular and circulating levels of glycoconjugates, lipoproteins, glucose metabolites, amino acids, creatine/creatinine, and polyamines within the fluid.
- The follicular fluids were also tested for oestradiol and progesterone contents through a radioimmunoassay.
Findings
- The dominant follicle saw a decrease in the intrafollicular content of alanine and lipoproteins during growth, whereas the concentrations of progesterone and oestradiol significantly increased.
- After inducing ovulation by injecting gonadotrophin, follicular maturation was characterized by a decrease in glycoconjugates, trimethylamines and acetate, decline in oestradiol concentration, and a further increase in the lipoprotein’s CH(3) groups and progesterone.
- The results showed a clear correlation between the intrafollicular content of alanine and oestradiol. A correlation between progesterone and glycoconjugates was also seen.
Conclusions
- This study signals that (1)H NMR can effectively identify specific changes in the biochemical composition of follicular fluid during follicular development.
- For the first time, this study demonstrated variation in several compounds (glycoconjugates, lipoproteins, glucose metabolites, amino acids, and polyamines) related to follicular growth and maturation.
- This research indicates that some of these variations could play an important role in follicular maturation and ovulation as well as the maturation of the oocyte and possible fertilization outcome.
Cite This Article
APA
Gu00e9rard N, Loiseau S, Duchamp G, Seguin F.
(2002).
Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H NMR).
Reproduction, 124(2), 241-248.
https://doi.org/10.1530/rep.0.1240241 Publication
Researcher Affiliations
- Equipe Reproduction Equine, PRC, INRA-Haras Nationaux, F-37380 Nouzilly, France. gerard@tours.inra.fr
MeSH Terms
- Alanine / metabolism
- Animals
- Estradiol / metabolism
- Female
- Follicular Fluid / metabolism
- Glucose / metabolism
- Glycoconjugates / metabolism
- Horses / metabolism
- Lipoproteins / metabolism
- Magnetic Resonance Spectroscopy
- Ovarian Follicle / growth & development
- Polyamines / metabolism
- Progesterone / metabolism
Citations
This article has been cited 17 times.- Bekkouche A, Miroud K, Mimoune N, Benamor B, Kaidi R, Benaissa MH. Follicular fluid and serum biochemical and hormonal profiles of normal and cystic dromedary camel breeds.. Vet World 2022 Nov;15(11):2511-2516.
- Luna-Garcu00eda LA, Meza-Herrera CA, Pu00e9rez-Maru00edn CC, Corona R, Luna-Orozco JR, Vu00e9liz-Deras FG, Delgado-Gonzalez R, Rodriguez-Venegas R, Rosales-Nieto CA, Bustamante-Andrade JA, Gutierrez-Guzman UN. Goats as Valuable Animal Model to Test the Targeted Glutamate Supplementation upon Antral Follicle Number, Ovulation Rate, and LH-Pulsatility.. Biology (Basel) 2022 Jul 6;11(7).
- Brinca AT, Ramalhinho AC, Sousa u00c2, Oliani AH, Breitenfeld L, Passarinha LA, Gallardo E. Follicular Fluid: A Powerful Tool for the Understanding and Diagnosis of Polycystic Ovary Syndrome.. Biomedicines 2022 May 27;10(6).
- Izquierdo D, Roura M, Pu00e9rez-Trujillo M, Soto-Heras S, Paramio MT. Fatty Acids and Metabolomic Composition of Follicular Fluid Collected from Environments Associated with Good and Poor Oocyte Competence in Goats.. Int J Mol Sci 2022 Apr 8;23(8).
- Read CC, Edwards L, Schrick N, Rhinehart JD, Payton RR, Campagna SR, Castro HF, Klabnik JL, Horn EJ, Moorey SE. Correlation between Pre-Ovulatory Follicle Diameter and Follicular Fluid Metabolome Profiles in Lactating Beef Cows.. Metabolites 2021 Sep 14;11(9).
- Zacharopoulos G, Emir U, Cohen Kadosh R. The cross-sectional interplay between neurochemical profile and brain connectivity.. Hum Brain Mapp 2021 Jun 15;42(9):2722-2733.
- Muccini AM, Tran NT, de Guingand DL, Philip M, Della Gatta PA, Galinsky R, Sherman LS, Kelleher MA, Palmer KR, Berry MJ, Walker DW, Snow RJ, Ellery SJ. Creatine Metabolism in Female Reproduction, Pregnancy and Newborn Health.. Nutrients 2021 Feb 2;13(2).
- Asampille G, Cheredath A, Joseph D, Adiga SK, Atreya HS. The utility of nuclear magnetic resonance spectroscopy in assisted reproduction.. Open Biol 2020 Nov;10(11):200092.
- Fernu00e1ndez-Hernu00e1ndez P, Su00e1nchez-Calabuig MJ, Garcu00eda-Maru00edn LJ, Bragado MJ, Gutiu00e9rrez-Adu00e1n A, Millet u00d3, Bruzzone C, Gonzu00e1lez-Fernu00e1ndez L, Macu00edas-Garcu00eda B. Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media.. Animals (Basel) 2020 May 19;10(5).
- Satuu00e9 K, Fazio E, Medica P. Can the Presence of Ovarian Corpus Luteum Modify the Hormonal Composition of Follicular Fluid in Mares?. Animals (Basel) 2020 Apr 9;10(4).
- Swelum AA, Saadeldin IM, Abdelnour SA, Ba-Awadh H, Abd El-Hack ME, Sheiha AM. Relationship between concentrations of macro and trace elements in serum and follicular, oviductal, and uterine fluids of the dromedary camel (Camelus dromedarius).. Trop Anim Health Prod 2020 May;52(3):1315-1324.
- Ahamad SR, Yaqoob SH, Khan A, Shakeel F. Metabolite profile and elemental determination of camel follicular fluid by GC-MS and ICP-MS.. Trop Anim Health Prod 2019 Nov;51(8):2447-2454.
- Guerreiro TM, Gonu00e7alves RF, Melo CFOR, de Oliveira DN, Lima EO, Visintin JA, de Achilles MA, Catharino RR. A Metabolomic Overview of Follicular Fluid in Cows.. Front Vet Sci 2018;5:10.
- Kim K, Bloom MS, Browne RW, Bell EM, Yucel RM, Fujimoto VY. Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients.. J Assist Reprod Genet 2017 Jan;34(1):1-10.
- Salazar-Ortiz J, Monget P, Guillaume D. The influence of nutrition on the insulin-like growth factor system and the concentrations of growth hormone, glucose, insulin, gonadotropins and progesterone in ovarian follicular fluid and plasma from adult female horses (Equus caballus).. Reprod Biol Endocrinol 2014 Jul 31;12:72.
- Filannino A, Stout TA, Gadella BM, Sostaric E, Pizzi F, Colenbrander B, Dell'Aquila ME, Minervini F. Dose-response effects of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm.. Reprod Biol Endocrinol 2011 Oct 5;9:134.
- Sarty GE, Kendall EJ, Adams GP, Pierson RA. Nuclear magnetic resonance spectroscopy of bovine ovarian follicular fluid at four selected times of the oestrous cycle.. Reprod Fertil Dev 2006;18(5):559-71.
Secure Payments
Satisfaction Guarantee
We offer a 60-day guarantee to ensure you are completely satisfied.
Fast & Free Shipping
We provide fast and free shipping on all orders over $40.