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Veterinary sciences2023; 10(2); 114; doi: 10.3390/vetsci10020114

Analytical Performance Evaluation of the New GEM® Premier™ 5000 in Comparison to the Epoc® Blood Gas Analyzer in Horses.

Abstract: Different blood gas analyzers are used in equine practice. Every machine needs to be validated, as they have not been designed for use in horses. The aim of this study was to compare the newly marketed GEM5000 machine to the formerly validated epoc machine for blood gas analysis in horses. In this prospective, non-blinded, comparative laboratory analyzer study, 43 equine blood samples were analyzed on both analyzers and values were compared between the two machines via Lin's concordance analysis, Passing-Bablok regression analysis and Bland-Altman plots. Duplicate measurements were conducted on the GEM5000 machine to evaluate precision. The GEM5000 failed to achieve the required precision for tHb, Hct and iCa, but achieved acceptable precision for all other parameters. Concordance correlation analysis revealed poor correlation for Na, Cl, iCa, K, Hct and tHb, while there was an at least moderate agreement for all other parameters. Passing-Bablok regression revealed significant constant bias for pCO, pO, Cl, and iCa and significant proportional bias for pCO, iCa and SO. Bland-Altman analysis revealed significant systematic bias for Na, Cl, iCa, K, Hct, tHb and SO. This study shows that while precision of the GEM5000 is good, values should not be used interchangeably with data obtained from other blood gas analyzers.
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Publication Date: 2023-02-03 PubMed ID: 36851418PubMed Central: PMC9966012DOI: 10.3390/vetsci10020114Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the efficiency of the new GEM5000 blood gas analyzer, as compared to the Epoc machine, specifically for use on horses. The study discovered that while the GEM5000 machine demonstrated overall good precision, it is not suitable to be used interchangeably with other blood gas analyzers due to significant biases in analyzing certain parameters.

Research Methodology

  • The study was a prospective, non-blinded, comparative laboratory analyzer research, focusing on two different blood gas analyzers, the GEM5000 and Epoc.
  • 43 equine blood samples were used in this study in order to apply the comparative analysis.
  • The blood samples were analyzed on both machines and the values derived were compared using different analytical methods; Lin’s Concordance Analysis, Passing-Bablok Regression Analysis and Bland-Altman Plots.
  • Duplicate measurements were conducted specifically on the GEM5000 machine to further evaluate its precision.

Research Findings

  • The GEM5000 blood gas analyzer failed to reach the required precision for tHb (total hemoglobin), Hct (Hematocrit), and iCa (Ionized Calcium).
  • However, for all other parameters, the GEM5000 machine achieved acceptable precision.
  • The Concordance Correlation Analysis revealed poor correlation for certain parameters (Na – Sodium, Cl – Chloride, iCa – Ionized Calcium, K – Potassium, Hct – Hematocrit, and tHb – total Hemoglobin).
  • Poor correlation in this context means less agreement between the values derived from the GEM5000 and the Epoc machine for those specific parameters.
  • At least moderate agreement was found for all other parameters.
  • Passing-Bablok regression analysis discovered significant constant bias for pCO2 (Partial Pressure of Carbon Dioxide), pO2 (Partial Pressure of Oxygen), Cl (Chloride), and iCa (Ionized Calcium) & significant proportional bias for pCO2 (Partial Pressure of Carbon Dioxide), iCa (Ionized Calcium) and SO2 (Oxygen Saturation).
  • Bland-Altman analysis revealed a significant systematic bias for sodium (Na), chloride (Cl), ionized calcium (iCa), potassium (K), hematocrit (Hct), total hemoglobin (tHb), and oxygen saturation (SO2).

Final Conclusion

  • The final verdict of the study revealed that while the GEM5000 has overall good precision, results derived from it should not be used interchangeably with data obtained from other blood gas analyzers.
  • This advice stems from the significant biases observed in specific parameters when analyzed by the GEM5000 machine as compared to the Epoc machine.

Cite This Article

APA
Sandersen C, Dmitrovic P, Dupont J, Cesarini C, Guyot H, Serteyn D, Kirsch K. (2023). Analytical Performance Evaluation of the New GEM® Premier™ 5000 in Comparison to the Epoc® Blood Gas Analyzer in Horses. Vet Sci, 10(2), 114. https://doi.org/10.3390/vetsci10020114

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 10
Issue: 2
PII: 114

Researcher Affiliations

Sandersen, Charlotte
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Dmitrovic, Petra
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Dupont, Julien
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Cesarini, Carla
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Guyot, Hugues
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Serteyn, Didier
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Kirsch, Katharina
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
  • German Olympic Committee for Equestrian Sports, 48231 Warendorf, Germany.

Conflict of Interest Statement

The authors declare no conflict of interest.

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