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Andrographolide Exerts Chondroprotective Activity in Equine Cartilage Explant and Suppresses Interleukin-1 β -Induced MMP-2 Expression in Equine Chondrocyte Culture.
Abstract: Cartilage erosion in degenerative joint diseases leads to lameness in affected horses. It has been reported that andrographolide from Andrographis paniculata inhibited cartilage matrix-degrading enzymes. This study aimed to explore whether this compound protects equine cartilage degradation in the explant culture model and to determine its effect on matrix metalloproteinase-2 (MMP-2) expression, a matrix-degrading enzyme, in equine chondrocyte culture. Equine articular cartilage explant culture was induced by 25 ng/mL interleukin-1β, a key inducer of cartilage degeneration, in cultures with or without andrographolide ranging from 10 to 50 μM. After 3-21 days, they were analyzed for the markers of cartilage degradation. It was found that interleukin-1β increased the release of sulfated glycosaminoglycans and hyaluronan from the explants into the culture media consistently with the decrease in uronic acid and collagen content in the cartilage explants. These catabolic effects were inhibited when cotreated with interleukin-1β and andrographolide. In primary equine chondrocytes, andrographolide suppressed interleukin-1β-induced MMP-2 mRNA expression and MMP-2 activity in the culture medium. These results confirmed the in vitro potent chondroprotective activities of this compound which were performed in cartilage explants and on a cellular level. These may indicate the application of andrographolide for therapeutic use in equine degenerative joint diseases.
Publication Date: 2014-10-29 PubMed ID: 27379277PubMed Central: PMC4897368DOI: 10.1155/2014/464136Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research article investigates the potential for a compound, Andrographolide, from the plant Andrographis paniculata, to protect horse cartilage from degradation and reduce the effects of the enzyme MMP-2 on cartilage, which plays a key role in cartilage degeneration. The study also proves the protective properties of the compound both on a cellular level and in cartilage explants.
Background and Objectives
- The study looks into the degenerative joint diseases in horses that lead to lameness due to cartilage erosion. The cartilage degradation is induced by the activity of matrix metalloproteinase-2 (MMP-2), a matrix-degrading enzyme.
- The team sought to explore if andrographolide, derived from the plant Andrographis paniculata, can inhibit cartilage matrix-degrading enzymes and preserve cartilage integrity.
Research Methodology
- Researchers used an equine articular cartilage explant culture, which was triggered by interleukin-1β (a known inducer of cartilage degeneration). They employed varying levels of andrographolide (from 10 to 50 μM) in the cultures.
- The research involved analyzing markers of cartilage degradation between 3 to 21 days.
Key Findings
- The interleukin-1β resulted in an increased release of sulfated glycosaminoglycans and hyaluronan from the explants into the culture media. This was accompanied by a decrease in uronic acid and collagen content in the cartilage explants, evidencing degradation.
- This degradation was inhibited when the cultures were cotreated with interleukin-1β and andrographolide, meaning andrographolide interfered with the cartilage degradation process.
- In primary equine chondrocytes, andrographolide suppressed interleukin-1β-induced MMP-2 mRNA expression and MMP-2 activity in the culture medium, hence providing in vitro evidence of its chondroprotective potential.
Implication of Findings
- The results attest to the potent chondroprotective activities of the compound andrographolide. This offers potential application in treating degenerative joint diseases in horses, though further research and trials would undoubtedly be needed to confirm efficacy and safety in vivo.
Cite This Article
APA
Tangyuenyong S, Viriyakhasem N, Peansukmanee S, Kongtawelert P, Ongchai S.
(2014).
Andrographolide Exerts Chondroprotective Activity in Equine Cartilage Explant and Suppresses Interleukin-1 β -Induced MMP-2 Expression in Equine Chondrocyte Culture.
Int Sch Res Notices, 2014, 464136.
https://doi.org/10.1155/2014/464136 Publication
Researcher Affiliations
- Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
- Thailand Excellence Centre for Tissue Engineering and Stem Cells, Department of Biochemistry and the Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
- Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
- Thailand Excellence Centre for Tissue Engineering and Stem Cells, Department of Biochemistry and the Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
- Thailand Excellence Centre for Tissue Engineering and Stem Cells, Department of Biochemistry and the Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
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Citations
This article has been cited 3 times.- Wu X, Fu Y, Ma J, Wang H, Li C, Zhu Y, Wang Q, Guo X, Zhang T, He A. Blocking calcium-MYC regulatory axis inhibits early dedifferentiation of chondrocytes and contributes to cartilage regeneration. Stem Cell Res Ther 2025 Jul 15;16(1):372.
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