Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru.
Abstract: In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication Date: 2014-10-30 PubMed ID: 25448393DOI: 10.1016/j.toxicon.2014.10.023Google Scholar: Lookup
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- Research Support
- Non-U.S. Gov't
Summary
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This research paper shows the creation of an anti-loxoscelic serum from a recombinant dermonecrotic protein derived from Brazilian Loxosceles intermedia spiders, showcasing its ability to neutralize lethal effects of venom from Peruvian Loxosceles laeta spiders.
Production of Anti-loxoscelic Serum
- The researchers initially worked to create an anti-loxoscelic serum. This was achieved by injecting horses with a recombinant protein known as rLiD1, which is derived from a kind of Brazilian spider, the Loxosceles intermedia.
- This process of immunization allowed for the presence of specific anti-rLiD1 antibodies in the horses, which were recognized for their ability to identify different species of Loxosceles venoms when tested by methods such as Western Blot and ELISA.
Effectiveness of the Serum
- The main objective of the study was to determine the neutralizing potential of the developed serum on the toxic effects of spider venom, specifically focusing on Loxosceles laeta species from Peru.
- The findings indicated that anti-rLiD1 serum effectively neutralized the harmful consequences of the Peruvian spider venom, thereby demonstrating its potential use for treating venomous bites.
Implications of the Study
- The study validated the argument that recombinant proteins could serve as excellent alternatives to crude venoms in the production of antivenom.
- This is a considerable achievement as it suggests a move away from the traditional method of producing antivenom, which involve the use of venom from live spiders – a complicated and potentially dangerous process.
- Furthermore, the use of recombinant proteins allows for more controlled production processes, potentially minimizing variations in the final product and improving the quality and efficacy of antivenoms.
Cite This Article
APA
Duarte CG, Bonilla C, Guimarães G, Machado de Avila RA, Mendes TM, Silva W, Tintaya B, Yarleque A, Chávez-Olórtegui C.
(2014).
Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru.
Toxicon, 93, 37-40.
https://doi.org/10.1016/j.toxicon.2014.10.023 Publication
Researcher Affiliations
- Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
- Instituto Nacional de Salud, Lima, Peru.
- Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
- Universidade do Extremo Sul Catarinense - UNESC, Criciúma, Santa Catarina, Brazil.
- Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
- Instituto Nacional de Salud, Lima, Peru.
- Instituto Nacional de Salud, Lima, Peru.
- Universidad Nacional Mayor de San Marcos, Lima, Peru.
- Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. Electronic address: olortegi@icb.ufmg.br.
MeSH Terms
- Animals
- Antivenins / immunology
- Antivenins / pharmacology
- Blotting, Western
- Brazil
- Cross Reactions
- Enzyme-Linked Immunosorbent Assay
- Horses
- Neutralization Tests
- Peru
- Phosphoric Diester Hydrolases / analysis
- Phosphoric Diester Hydrolases / chemistry
- Phosphoric Diester Hydrolases / metabolism
- Recombinant Proteins / metabolism
- Species Specificity
- Spider Venoms / chemistry
- Spider Venoms / enzymology
Citations
This article has been cited 9 times.- Miranda ALS, Antunes BC, Minozzo JC, Lima SA, Botelho AFM, Campos MTG, Chávez-Olórtegui CD, Soto-Blanco B. Clinical Effects of the Immunization Protocol Using Loxosceles Venom in Naïve Horses.. Toxins (Basel) 2022 May 13;14(5).
- Gremski LH, Matsubara FH, Polli NLC, Antunes BC, Schluga PHC, da Justa HC, Minozzo JC, Wille ACM, Senff-Ribeiro A, Veiga SS. Prospective Use of Brown Spider Venom Toxins as Therapeutic and Biotechnological Inputs.. Front Mol Biosci 2021;8:706704.
- Fingermann M, de Roodt AR, Cascone O, Miranda MV. Biotechnological potential of Phospholipase D for Loxosceles antivenom development.. Toxicon X 2020 Jun;6:100036.
- Karim-Silva S, Becker-Finco A, Jiacomini IG, Boursin F, Leroy A, Noiray M, de Moura J, Aubrey N, Billiald P, Alvarenga LM. Loxoscelism: Advances and Challenges in the Design of Antibody Fragments with Therapeutic Potential.. Toxins (Basel) 2020 Apr 16;12(4).
- Grashof D, Zdenek CN, Dobson JS, Youngman NJ, Coimbra F, Benard-Valle M, Alagon A, Fry BG. A Web of Coagulotoxicity: Failure of Antivenom to Neutralize the Destructive (Non-Clotting) Fibrinogenolytic Activity of Loxosceles and Sicarius Spider Venoms.. Toxins (Basel) 2020 Jan 30;12(2).
- Boia-Ferreira M, Moreno KG, Basílio ABC, da Silva LP, Vuitika L, Soley B, Wille ACM, Donatti L, Barbaro KC, Chaim OM, Gremski LH, Veiga SS, Senff-Ribeiro A. TCTP from Loxosceles Intermedia (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism.. Cells 2019 Nov 22;8(12).
- Chaves-Moreira D, Matsubara FH, Schemczssen-Graeff Z, De Bona E, Heidemann VR, Guerra-Duarte C, Gremski LH, Chávez-Olórtegui C, Senff-Ribeiro A, Chaim OM, Arni RK, Veiga SS. Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics.. Toxins (Basel) 2019 Jun 19;11(6).
- Calabria PAL, Shimokava-Falcao LHAL, Colombini M, Moura-da-Silva AM, Barbaro KC, Faquim-Mauro EL, Magalhaes GS. Design and Production of a Recombinant Hybrid Toxin to Raise Protective Antibodies Against Loxosceles Spider Venom.. Toxins (Basel) 2019 Feb 12;11(2).
- Bermúdez-Méndez E, Fuglsang-Madsen A, Føns S, Lomonte B, Gutiérrez JM, Laustsen AH. Innovative Immunization Strategies for Antivenom Development.. Toxins (Basel) 2018 Nov 2;10(11).
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