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Clinical and vaccine immunology : CVI2011; 18(9); 1562-1567; doi: 10.1128/CVI.05123-11

Antibody profiling of Borrelia burgdorferi infection in horses.

Abstract: Infection with Borrelia burgdorferi is common in horses and ponies from the New England and mid-Atlantic regions of the United States. Here, we evaluated luciferase immunoprecipitation systems (LIPS) for profiling antibody responses against three different antigenic targets for the diagnosis of equine B. burgdorferi infection. LIPS testing of horse serum samples suspected of Lyme infection revealed that approximately 75% of the horse samples (114/159) were seropositive against the synthetic VOVO antigen, comprising repeated immunodominant C6 epitopes as well as OspC immunodominant epitopes. A comparison of VOVO and immunofluorescence assays (IFA) showed that 51% of the samples were positive in both assays (VOVO(+)/IFA(+)), 13% were VOVO(-)/IFA(+), 21% were VOVO(+)/IFA(-), and 15% were negative in both. To further understand humoral responses to B. burgdorferi and reconcile the diagnostic differences between IFA and VOVO, two additional B. burgdorferi LIPS tests were performed with DbpA and DbpB. Robust seropositive antibody responses against DbpA and/or DbpB were detected in 98% (79/81) of the VOVO(+)/IFA(+) and 93% (50/54) of the discrepant samples. Additionally, some of the samples negative by both VOVO and IFA showed immunoreactivity against DbpA and/or DbpB. Overall, 94% of the suspected horse samples were seropositive by LIPS, and heat map analysis revealed that seropositive samples often were immunoreactive with at least two of the three antigens. These results suggest that LIPS tests employing multiple recombinant antigens offer a promising approach for the evaluation of antibody responses in Lyme disease.
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Publication Date: 2011-07-20 PubMed ID: 21775514PubMed Central: PMC3165210DOI: 10.1128/CVI.05123-11Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the application of luciferase immunoprecipitation systems (LIPS) for determining the presence of Borrelia burgdorferi infection, a common bacterial infection, in horses. This was conducted by profiling the horses’ antibody responses to certain antigen targets. The study indicated a high success rate for LIPS tests, suggesting them as an effective method for diagnosing this infection in horses.

Experiment and Findings

The researchers used LIPS test to evaluate the antibody response of horse serum samples suspected of having Lyme disease against three different antigenic targets. The primary findings from the experiment are as follows:

  • About 75% of the horse serum samples were seropositive against the synthetic VOVO antigen, indicating the presence of the B. burgdorferi infection.
  • Further comparison using VOVO and immunofluorescence assays (IFA) revealed discrepancies in the results. About 51% of the samples were positive in both assays (VOVO(+)/IFA(+)), while the rest demonstrated mixed results.
  • Two additional B. burgdorferi LIPS tests were performed using DbpA and DbpB to reconcile these discrepancies and better understand the humoral responses to B. burgdorferi. Robust seropositive antibody responses were detected against DbpA and/or DbpB in the majority of the VOVO(+)/IFA(+) and discrepant samples.
  • Overall, 94% of the suspected horse serum samples were seropositive by LIPS and often showed immunoreactivity with at least two of the three antigens.

Interpretation and Potential Implications

The study results reveal the efficacy of LIPS in diagnosing B. burgdorferi infection in horses. This is significant as it provides an effective means of identifying the infection, particularly in regions where this disease is prevalent.

  • The combined use of the VOVO antigen and the immunofluorescence assays (IFA) successfully diagnosed the infection in 51% of the cases, indicating a promising approach to resolving the discrepancies in the diagnosis of the disease.
  • The additional LIPS tests with DbpA and DbpB detected antibody responses against these antibodies, potentially improving the diagnosis and management of B. burgdorferi infection.
  • A notable aspect is the prevalence of seropositive results by LIPS, which suggests that this approach can be widely implemented as a diagnostic tool for Lyme disease.

In conclusion, the research suggests that the LIPS tests offer a promising approach in the evaluation of antibody responses for Lyme disease diagnosis, providing a potential leap in effectively managing Lyme disease in horses.

Cite This Article

APA
Burbelo PD, Bren KE, Ching KH, Coleman A, Yang X, Kariu T, Iadarola MJ, Pal U. (2011). Antibody profiling of Borrelia burgdorferi infection in horses. Clin Vaccine Immunol, 18(9), 1562-1567. https://doi.org/10.1128/CVI.05123-11

Publication

Clinical and vaccine immunology : CVI
ISSN: 1556-679X
NlmUniqueID: 101252125
Country: United States
Language: English
Volume: 18
Issue: 9
Pages: 1562-1567

Researcher Affiliations

Burbelo, Peter D
  • National Institutes of Health, Bethesda, MD 20892, USA. burbelop@nidcr.nih.gov
Bren, Kathleen E
    Ching, Kathryn H
      Coleman, Adam
        Yang, Xiuli
          Kariu, Toru
            Iadarola, Michael J
              Pal, Utpal

                MeSH Terms

                • Adhesins, Bacterial / genetics
                • Adhesins, Bacterial / immunology
                • Adhesins, Bacterial / metabolism
                • Animals
                • Antibodies, Bacterial / blood
                • Antigens, Bacterial / genetics
                • Antigens, Bacterial / immunology
                • Antigens, Bacterial / metabolism
                • Bacterial Proteins / genetics
                • Bacterial Proteins / immunology
                • Bacterial Proteins / metabolism
                • Borrelia burgdorferi / genetics
                • Borrelia burgdorferi / immunology
                • Fluorescent Antibody Technique
                • Horse Diseases / diagnosis
                • Horse Diseases / immunology
                • Horse Diseases / microbiology
                • Horses
                • Immunoprecipitation
                • Luciferases / genetics
                • Luciferases / metabolism
                • Lyme Disease / diagnosis
                • Lyme Disease / veterinary
                • Recombinant Proteins / genetics
                • Recombinant Proteins / immunology

                Grant Funding

                • AR055323 / NIAMS NIH HHS
                • R01 AI080615 / NIAID NIH HHS
                • AI080615 / NIAID NIH HHS
                • R56 AI080615 / NIAID NIH HHS
                • R03 AR055323 / NIAMS NIH HHS

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