Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis.
Abstract: The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.
Publication Date: 2010-07-14 PubMed ID: 20624349
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explores the presence of antibodies against Trypanosoma evansi in animals in Nhecolandia, a subregion of Pantanal Sul-mato-grossense, using different testing methods. The study utilised experimental and naturally infected animal sera to identify major polypeptides of T. evansi, contributing towards more precise diagnostic processes for T. evansi infection.
Overview of Research Methods
- Researchers obtained serum samples from various animals – bovines, horses, dogs, and free-ranging coatis.
- The presence of T. evansi antibodies in these samples was investigated via enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT).
- A technique known as Western blotting was used to identify major polypeptides of T. evansi, recognized by sera from experimentally and naturally infected hosts.
Results of the Research
- None of the bovines tested positive for T. evansi antibodies.
- 16 dogs (29%) and 23 horses (23.4%) tested positive by ELISA.
- 8 coatis (25%) were positive using IFAT.
- Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa.
- Polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. These were mainly recognized in the chronic phase of infection.
- The antigen with an apparent molecular weight of 66 kDa, which was revealed by antibodies from all experimental animals, was also recognized in the sera of horses and dogs from the Pantanal.
- The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and has been deemed a potential candidate for use in specific diagnosis of T. evansi infection.
Significance of the Research
The research contributes to our understanding of the presence of T. evansi antibodies in various types of animals. It highlights the potential of major polypeptides, specifically the 48-46 kDa polypeptide, in the diagnosis of T. evansi infection. This new knowledge could potentially enhance diagnostic precision and contribute to the control of this infectious disease.
Cite This Article
APA
Aquino LP, Machado RZ, Lemos KR, Marques LC, Garcia MV, Borges GP.
(2010).
Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis.
Rev Bras Parasitol Vet, 19(2), 112-118.
Publication
Researcher Affiliations
- Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista -- UNESP - SP, Brazil.
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Antigens, Protozoan / blood
- Cattle / blood
- Dogs / blood
- Horses / blood
- Raccoons / blood
- Trypanosoma / classification
- Trypanosoma / immunology
Citations
This article has been cited 7 times.- Reck C, Menin Á, Batista F, Meira Santos PO, Miletti LC. Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil. Curr Res Parasitol Vector Borne Dis 2021;1:100024.
- Sharma D, Gupta S, Sethi K, Kumar S, Kumar R. Seroprevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic zones of Himachal Pradesh, India. Trop Anim Health Prod 2022 Jan 15;54(1):60.
- Aregawi WG, Agga GE, Abdi RD, Büscher P. Systematic review and meta-analysis on the global distribution, host range, and prevalence of Trypanosoma evansi. Parasit Vectors 2019 Jan 31;12(1):67.
- Porfirio GEO, Santos FM, de Macedo GC, Barreto WTG, Campos JBV, Meyers AC, André MR, Perles L, de Oliveira CE, Xavier SCDC, Andrade GB, Jansen AM, Herrera HM. Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border. Int J Parasitol Parasites Wildl 2018 Dec;7(3):398-404.
- Santos FM, de Macedo GC, Barreto WTG, Oliveira-Santos LGR, Garcia CM, Mourão GM, Edith de Oliveira Porfírio G, Domenis Marino E, Rogério André M, Perles L, Elisei de Oliveira C, Braziliano de Andrade G, Jansen AM, Miraglia Herrera H. Outcomes of Trypanosoma cruzi and Trypanosoma evansi infections on health of Southern coati (Nasua nasua), crab-eating fox (Cerdocyon thous), and ocelot (Leopardus pardalis) in the Brazilian Pantanal. PLoS One 2018;13(8):e0201357.
- Raftery AG, Gummery L, Garcia K, Mohite D, Capewell P, Sutton DGM. Equine trypanosomiasis, a systematic review and meta-analyses: Prevalence, morbidity and mortality. Equine Vet J 2026 Mar;58(2):291-319.
- Sousa JA, Miranda LM, Coutinho DJB, Costa TF, Costa SP, Freitas ÚS, Costa FB, Machado RZ, Nogueira RMS, Costa APD. Trypanosoma evansi in Brazil: first evidence of infection in equines from Northeast region. Rev Bras Parasitol Vet 2025;34(2):e000125.
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