Antigenic mapping of the envelope proteins of equine infectious anemia virus: identification of a neutralization domain and a conserved region on glycoprotein 90.
Abstract: Monoclonal antibodies (MCAbs) were used to dissect the antigenic sites of the surface glycoproteins of the prototype cell-adapted Wyoming strain of equine infectious anemia virus (EIAV). Serologic reactivities of these MCAbs were determined by ELISA, additive ELISA, competitive ELISA, and Western blot assays. The results indicated that antigenic reactivity of gp90 was localized on at least four distinct epitopes, two of which were important in neutralization. Our studies also revealed that these epitopes were localized on overlapping antigenic sites on gp90. On the other hand, only two distinct non-overlapping epitopes were identified on gp45. Competitive binding studies of neutralizing MCAbs and reference EIA-positive horse serum delineated the presence of a neutralization domain on gp90 that appears to be immunodominant both in naturally infected horses and in mice immunized with EIAV. Limited proteolytic fragmentation of the gp90 component of several serologically distinct EIAV isolates produced common 12K immunoreactive fragments that contained a conserved epitope. These results indicate the occurrence of conserved antigenic regions on EIAV glycoproteins as well as a neutralization domain on gp90, which can be used as potential targets for vaccine development.
Publication Date: 1988-01-01 PubMed ID: 2450529DOI: 10.1007/BF01322170Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
- Antibodies
- Antigen
- Diagnosis
- Disease Diagnosis
- Disease Treatment
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Infectious Anemia
- Immunization
- Immunology
- In Vitro Research
- Infectious Disease
- Laboratory Methods
- Monoclonal Antibodies
- Vaccine development
- Veterinary Medicine
- Virology
- Virus
- Western Blot
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study investigates the antigens present on the surface proteins of the equine infectious anemia virus (EIAV). Researchers used a variety of tests to understand the reactivity of these antigens and identified that the antigen reactivity was located in four distinct epitopes. These findings may be significant for developing vaccines against EIAV.
Objective of the Research
- The primary objective of this study was to identify the locations of the antigenic sites – areas that can elicit an immune response – on the surface glycoproteins of EIAV.
- Researchers aimed to determine whether specific elements on these glycoproteins could serve as potential targets for vaccine development.
Methods Used in the Study
- The researchers performed multiple tests to pinpoint antigenic reactivity locations, including ELISA, additive ELISA, competitive ELISA, and Western blot assays using Monoclonal antibodies.
- Competitive binding studies were conducted to outline the presence of a neutralization domain – a region that can neutralize the effect of the virus – on gp90.
- Limited proteolytic fragmentation was carried out to identify common immunoreactive fragments and to find conserved epitopes.
Findings of the Research
- The results of the tests revealed that the antigenic reactivity of gp90 was located within at least four distinct epitopes, two of which played a significant role in neutralizing the virus.
- A neutralization domain was discovered on gp90, which appeared to be immunodominant both in naturally infected horses and in mice immunized with EIAV.
- Analysis of proteolytic fragments of the gp90 component demonstrated that it contained a conserved epitope – an epitope that maintains its structure and characteristics across different virus isolates, hence can potentially serve as a reliable target for vaccines.
Significance of the Study
- This research aids in increasing our understanding of EIAV protein structure and its interaction with the immune system.
- The study’s findings point out the possible target areas on EIAV glycoproteins, such as a neutralization domain on gp90 and conserved antigenic regions, that can be exploited for vaccine development.
Cite This Article
APA
Hussain KA, Issel CJ, Schnorr KL, Rwambo PM, West M, Montelaro RC.
(1988).
Antigenic mapping of the envelope proteins of equine infectious anemia virus: identification of a neutralization domain and a conserved region on glycoprotein 90.
Arch Virol, 98(3-4), 213-224.
https://doi.org/10.1007/BF01322170 Publication
Researcher Affiliations
- Department of Veterinary Microbiology and Parasitology, Louisiana State University School of Veterinary Medicine, Baton Rouge.
MeSH Terms
- Antibodies, Monoclonal / immunology
- Antibodies, Viral / immunology
- Antigens, Viral / immunology
- Enzyme-Linked Immunosorbent Assay
- Epitopes / immunology
- Glycoproteins / immunology
- Infectious Anemia Virus, Equine / immunology
- Neutralization Tests
- Viral Envelope Proteins / immunology
Grant Funding
- CA-38851 / NCI NIH HHS
References
This article includes 18 references
- Montelaro RC, West M, Issel CJ. Antigenic reactivity of the major glycoprotein of equine infectious anemia virus, a retrovirus.. Virology 1984 Jul 30;136(2):368-74.
- Montelaro RC, Parekh B, Orrego A, Issel CJ. Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus.. J Biol Chem 1984 Aug 25;259(16):10539-44.
- Orrego A, Issel CJ, Montelaro RC, Adams WV Jr. Virulence and in vitro growth of a cell-adapted strain of equine infectious anemia virus after serial passage in ponies.. Am J Vet Res 1982 Sep;43(9):1556-60.
- Issel CJ, Coggins L. Equine infectious anemia: current knowledge.. J Am Vet Med Assoc 1979 Apr 1;174(7):727-33.
- Clegg JC. Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using concanavalin A and peroxidase: application to arenavirus and flavivirus proteins.. Anal Biochem 1982 Dec;127(2):389-94.
- Hussain KA, Issel CJ, Schnorr KL, Rwambo PM, Montelaro RC. Antigenic analysis of equine infectious anemia virus (EIAV) variants by using monoclonal antibodies: epitopes of glycoprotein gp90 of EIAV stimulate neutralizing antibodies.. J Virol 1987 Oct;61(10):2956-61.
- Lefrancois L. Protection against lethal viral infection by neutralizing and nonneutralizing monoclonal antibodies: distinct mechanisms of action in vivo.. J Virol 1984 Jul;51(1):208-14.
- Montelaro RC, Lohrey N, Parekh B, Blakeney EW, Issel CJ. Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus.. J Virol 1982 Jun;42(3):1029-38.
- Burnette WN. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.. Anal Biochem 1981 Apr;112(2):195-203.
- Montelaro RC, West M, Issel CJ. Isolation of equine infectious anemia virus glycoproteins. Lectin affinity chromatography procedures for high avidity glycoproteins.. J Virol Methods 1983 Jun;6(6):337-46.
- McGuire TC, Crawford TB, Henson JB. Equine infectious anemia: detection of infections virus-antibody complexes in the serum.. Immunol Commun 1972;1(6):545-51.
- Gallo RC. The AIDS virus.. Sci Am 1987 Jan;256(1):46-56.
- Parekh B, Issel CJ, Montelaro RC. Equine infectious anemia virus, a putative lentivirus, contains polypeptides analogous to prototype-C oncornaviruses.. Virology 1980 Dec;107(2):520-5.
- Payne S, Parekh B, Montelaro RC, Issel CJ. Genomic alterations associated with persistent infections by equine infectious anaemia virus, a retrovirus.. J Gen Virol 1984 Aug;65 ( Pt 8):1395-9.
- Friguet B, Djavadi-Ohaniance L, Pages J, Bussard A, Goldberg M. A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site. Application to hybridomas specific for the beta 2-subunit of Escherichia coli tryptophan synthase.. J Immunol Methods 1983 Jun 10;60(3):351-8.
- Payne SL, Salinovich O, Nauman SM, Issel CJ, Montelaro RC. Course and extent of variation of equine infectious anemia virus during parallel persistent infections.. J Virol 1987 Apr;61(4):1266-70.
- Salinovich O, Payne SL, Montelaro RC, Hussain KA, Issel CJ, Schnorr KL. Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection.. J Virol 1986 Jan;57(1):71-80.
- Malmquist WA, Barnett D, Becvar CS. Production of equine infectious anemia antigen in a persistently infected cell line.. Arch Gesamte Virusforsch 1973;42(4):361-70.
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