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Home / Equine Research Bank / J Biol Chem / Antigenic variation during persistent infection by equine in...
The Journal of biological chemistry1984; 259(16); 10539-10544;

Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus.

Abstract: The recurrent nature of equine infectious anemia has been attributed to relatively rapid antigenic variations in equine infectious anemia virus (EIAV) during persistent infection under selective immune pressures. This model was tested by serological and biochemical analysis of virus isolates recovered from separate febrile episodes in two experimentally infected ponies. Neutralization assays employing immune sera from the experimentally infected ponies demonstrated that distinct antigenic strains of virus predominate during sequential febrile episodes in a single pony. Analysis of the test strains of EIAV by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed varying electrophoretic mobilities for the respective virion glycoproteins gp90 and gp45. Furthermore, peptide mapping comparisons demonstrated structural variations between the gp90 components of the various strains. In contrast, the respective internal proteins of the virus strains displayed identical electrophoretic mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and, in general, produced identical tryptic peptide maps. The observed differences in glycoprotein electrophoretic mobility and peptide maps were highly reproducible and did not vary with repeated passage of the virus strains in cell culture. Thus, these results demonstrate the occurrence of glycoprotein-specific structural variations during persistent infection by EIAV and support the concept of antigenic variation in this retrovirus. This capacity to alter envelope glycoprotein structure, previously reported for visna virus, may represent an important mechanism of retrovirus persistence in the presence of immune responses by the animal host.
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Publication Date: 1984-08-25 PubMed ID: 6206055
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  • U.S. Gov't
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how the equine infectious anemia virus (EIAV), a horse-disease causing retrovirus, undergoes antigenic changes during a persistent infection, aiding its survival against the host’s immune responses.

Study Details and Methodology

In order to understand the virus better, experiments were performed on two ponies that were infected with EIAV. Both biochemical and serological testing methods were used to analyze the virus samples that were obtained from these ponies. The samples were collected during different febrile episodes in the ponies to examine if there were significant antigenic variations occurring over time in a single host.

  • Serological testing was done by performing neutralization assays using immune sera from the infected ponies. The aim was to check if different antigenic strains prevailed in sequential febrile phases within one pony.
  • Simultaneously, the EIAV samples were analyzed biochemically via sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The goal was to determine differences in the electrophoretic movement of specific viral glycoproteins, gp90 and gp45.
  • Peptide mapping comparisons were also made to indicate structural variations between the gp90 components of various virus strains.

Results

The analysis of the virus strains through neutralization assays and electrophoresis highlighted variable characteristics in distinct antigenic strains within one pony. In regards to the respective virion glycoproteins gp90 and gp45, different electrophoretic mobilities were observed. Moreover, peptide mapping demonstrated the existence of structural differences among the gp90 components across various strains.

The internal proteins of the virus strains, however, did not display such variability. They showed identical electrophoretic mobilities and generated identical tryptic peptide maps. All these differences were noted to be highly reproducible and remained constant even with repeated growth of the virus strains in cell culture.

Conclusion

The research concludes that EIAV, during a persistent infection, demonstrates glycoprotein-specific structural variations. These observations confirm the hypothesis of antigenic variation within this retrovirus. The ability to alter envelope glycoprotein structure, which has been previously reported in viruses such as visna, could be an essential mechanism aiding their persistence against immune responses in the host body.

Cite This Article

APA
Montelaro RC, Parekh B, Orrego A, Issel CJ. (1984). Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus. J Biol Chem, 259(16), 10539-10544.

Publication

The Journal of biological chemistry
ISSN: 0021-9258
NlmUniqueID: 2985121R
Country: United States
Language: English
Volume: 259
Issue: 16
Pages: 10539-10544

Researcher Affiliations

Montelaro, R C
    Parekh, B
      Orrego, A
        Issel, C J

          MeSH Terms

          • Animals
          • Antigens, Viral / analysis
          • Antigens, Viral / genetics
          • Electrophoresis, Polyacrylamide Gel
          • Epitopes / analysis
          • Equine Infectious Anemia / immunology
          • Equine Infectious Anemia / microbiology
          • Genetic Variation
          • Glycoproteins / analysis
          • Horses
          • Infectious Anemia Virus, Equine / immunology
          • Peptide Fragments / analysis
          • Trypsin

          Grant Funding

          • AI-17594 / NIAID NIH HHS

          Citations

          This article has been cited 75 times.
          1. Waheed AA, Zhu Y, Agostino E, Naing L, Hikichi Y, Soheilian F, Yoo SW, Song Y, Zhang P, Slusher BS, Haughey NJ, Freed EO. Neutral sphingomyelinase 2 is required for HIV-1 maturation.. Proc Natl Acad Sci U S A 2023 Jul 11;120(28):e2219475120.
            doi: 10.1073/pnas.2219475120pubmed: 37406093google scholar: lookup
          2. Hu Z, Guo K, Du C, Sun J, Naletoski I, Chu X, Lin Y, Wang X, Barrandeguy M, Samuel M, Wang W, Lau PI, Wernery U, Raghavan R, Wang X. Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia.. Appl Microbiol Biotechnol 2023 May;107(10):3305-3317.
            doi: 10.1007/s00253-023-12504-5pubmed: 37039847google scholar: lookup
          3. Sette P, Nagashima K, Piper RC, Bouamr F. Ubiquitin conjugation to Gag is essential for ESCRT-mediated HIV-1 budding.. Retrovirology 2013 Jul 29;10:79.
            doi: 10.1186/1742-4690-10-79pubmed: 23895345google scholar: lookup
          4. Sette P, Dussupt V, Bouamr F. Identification of the HIV-1 NC binding interface in Alix Bro1 reveals a role for RNA.. J Virol 2012 Nov;86(21):11608-15.
            doi: 10.1128/JVI.01260-12pubmed: 22896625google scholar: lookup
          5. Bello NF, Dussupt V, Sette P, Rudd V, Nagashima K, Bibollet-Ruche F, Chen C, Montelaro RC, Hahn BH, Bouamr F. Budding of retroviruses utilizing divergent L domains requires nucleocapsid.. J Virol 2012 Apr;86(8):4182-93.
            doi: 10.1128/JVI.07105-11pubmed: 22345468google scholar: lookup
          6. Patel JR, Heldens JG, Bakonyi T, Rusvai M. Important mammalian veterinary viral immunodiseases and their control.. Vaccine 2012 Feb 27;30(10):1767-81.
            doi: 10.1016/j.vaccine.2012.01.014pubmed: 22261411google scholar: lookup
          7. Cappelli K, Capomaccio S, Cook FR, Felicetti M, Marenzoni ML, Coppola G, Verini-Supplizi A, Coletti M, Passamonti F. Molecular detection, epidemiology, and genetic characterization of novel European field isolates of equine infectious anemia virus.. J Clin Microbiol 2011 Jan;49(1):27-33.
            doi: 10.1128/JCM.01311-10pubmed: 21084503google scholar: lookup
          8. Mascola JR. The cat and mouse of HIV-1 antibody escape.. PLoS Pathog 2009 Sep;5(9):e1000592.
            doi: 10.1371/journal.ppat.1000592pubmed: 19763273google scholar: lookup
          9. Chung C, Mealey RH, McGuire TC. Evaluation of high functional avidity CTL to Gag epitope clusters in EIAV carrier horses.. Virology 2005 Nov 25;342(2):228-39.
            doi: 10.1016/j.virol.2005.07.033pubmed: 16139857google scholar: lookup
          10. Fraser DG, Leib SR, Zhang BS, Mealey RH, Brown WC, McGuire TC. Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge.. Clin Diagn Lab Immunol 2005 Aug;12(8):983-93.
            doi: 10.1128/CDLI.12.8.983-993.2005pubmed: 16085917google scholar: lookup
          11. Jin S, Chen C, Montelaro RC. Equine infectious anemia virus Gag p9 function in early steps of virus infection and provirus production.. J Virol 2005 Jul;79(14):8793-801.
            doi: 10.1128/JVI.79.14.8793-8801.2005pubmed: 15994773google scholar: lookup
          12. Paré J, Simard C. Comparison of commercial enzyme-linked immunosorbent assays and agar gel immunodiffusion tests for the serodiagnosis of equine infectious anemia.. Can J Vet Res 2004 Oct;68(4):254-8.
            pubmed: 15581219
          13. Chung C, Mealey RH, McGuire TC. CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins.. Virology 2004 Sep 15;327(1):144-54.
            doi: 10.1016/j.virol.2004.06.035pubmed: 15327905google scholar: lookup
          14. Payne SL, Pei XF, Jia B, Fagerness A, Fuller FJ. Influence of long terminal repeat and env on the virulence phenotype of equine infectious anemia virus.. J Virol 2004 Mar;78(5):2478-85.
            doi: 10.1128/jvi.78.5.2478-2485.2004pubmed: 14963146google scholar: lookup
          15. Chen C, Weisz OA, Stolz DB, Watkins SC, Montelaro RC. Differential effects of actin cytoskeleton dynamics on equine infectious anemia virus particle production.. J Virol 2004 Jan;78(2):882-91.
            doi: 10.1128/jvi.78.2.882-891.2004pubmed: 14694119google scholar: lookup
          16. Chen C, Montelaro RC. Characterization of RNA elements that regulate gag-pol ribosomal frameshifting in equine infectious anemia virus.. J Virol 2003 Oct;77(19):10280-7.
            doi: 10.1128/jvi.77.19.10280-10287.2003pubmed: 12970412google scholar: lookup
          17. Mealey RH, Zhang B, Leib SR, Littke MH, McGuire TC. Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus.. Virology 2003 Sep 1;313(2):537-52.
            doi: 10.1016/s0042-6822(03)00344-1pubmed: 12954220google scholar: lookup
          18. Fraser DG, Oaks JL, Brown WC, McGuire TC. Identification of broadly recognized, T helper 1 lymphocyte epitopes in an equine lentivirus.. Immunology 2002 Mar;105(3):295-305.
            doi: 10.1046/j.0019-2805.2001.01370.xpubmed: 11918691google scholar: lookup
          19. Chen C, Li F, Montelaro RC. Functional roles of equine infectious anemia virus Gag p9 in viral budding and infection.. J Virol 2001 Oct;75(20):9762-70.
            doi: 10.1128/JVI.75.20.9762-9770.2001pubmed: 11559809google scholar: lookup
          20. Belshan M, Harris ME, Shoemaker AE, Hope TJ, Carpenter S. Biological characterization of Rev variation in equine infectious anemia virus.. J Virol 1998 May;72(5):4421-6.
            doi: 10.1128/JVI.72.5.4421-4426.1998pubmed: 9557734google scholar: lookup
          21. Payne SL, Qi XM, Shao H, Dwyer A, Fuller FJ. Disease induction by virus derived from molecular clones of equine infectious anemia virus.. J Virol 1998 Jan;72(1):483-7.
            doi: 10.1128/JVI.72.1.483-487.1998pubmed: 9420249google scholar: lookup
          22. Leroux C, Issel CJ, Montelaro RC. Novel and dynamic evolution of equine infectious anemia virus genomic quasispecies associated with sequential disease cycles in an experimentally infected pony.. J Virol 1997 Dec;71(12):9627-39.
            doi: 10.1128/JVI.71.12.9627-9639.1997pubmed: 9371627google scholar: lookup
          23. Zheng YH, Sentsui H, Nakaya T, Kono Y, Ikuta K. In vivo dynamics of equine infectious anemia viruses emerging during febrile episodes: insertions/duplications at the principal neutralizing domain.. J Virol 1997 Jul;71(7):5031-9.
            doi: 10.1128/JVI.71.7.5031-5039.1997pubmed: 9188568google scholar: lookup
          24. Hammond SA, Cook SJ, Lichtenstein DL, Issel CJ, Montelaro RC. Maturation of the cellular and humoral immune responses to persistent infection in horses by equine infectious anemia virus is a complex and lengthy process.. J Virol 1997 May;71(5):3840-52.
            doi: 10.1128/JVI.71.5.3840-3852.1997pubmed: 9094660google scholar: lookup
          25. Lichtenstein DL, Issel CJ, Montelaro RC. Genomic quasispecies associated with the initiation of infection and disease in ponies experimentally infected with equine infectious anemia virus.. J Virol 1996 Jun;70(6):3346-54.
            doi: 10.1128/JVI.70.6.3346-3354.1996pubmed: 8648664google scholar: lookup
          26. Sellon DC, Walker KM, Russell KE, Perry ST, Covington P, Fuller FJ. Equine infectious anemia virus replication is upregulated during differentiation of blood monocytes from acutely infected horses.. J Virol 1996 Jan;70(1):590-4.
            doi: 10.1128/JVI.70.1.590-594.1996pubmed: 8523576google scholar: lookup
          27. Burns DP, Collignon C, Desrosiers RC. Simian immunodeficiency virus mutants resistant to serum neutralization arise during persistent infection of rhesus monkeys.. J Virol 1993 Jul;67(7):4104-13.
            doi: 10.1128/JVI.67.7.4104-4113.1993pubmed: 8510218google scholar: lookup
          28. Beisel CE, Edwards JF, Dunn LL, Rice NR. Analysis of multiple mRNAs from pathogenic equine infectious anemia virus (EIAV) in an acutely infected horse reveals a novel protein, Ttm, derived from the carboxy terminus of the EIAV transmembrane protein.. J Virol 1993 Feb;67(2):832-42.
            doi: 10.1128/JVI.67.2.832-842.1993pubmed: 8419648google scholar: lookup
          29. Carvalho M, Kirkland M, Derse D. Protein interactions with DNA elements in variant equine infectious anemia virus enhancers and their impact on transcriptional activity.. J Virol 1993 Nov;67(11):6586-95.
            doi: 10.1128/JVI.67.11.6586-6595.1993pubmed: 8411361google scholar: lookup
          30. Sellon DC. Equine infectious anemia.. Vet Clin North Am Equine Pract 1993 Aug;9(2):321-36.
            doi: 10.1016/s0749-0739(17)30399-1pubmed: 8395326google scholar: lookup
          31. Siebelink KH, Rimmelzwaan GF, Bosch ML, Meloen RH, Osterhaus AD. A single amino acid substitution in hypervariable region 5 of the envelope protein of feline immunodeficiency virus allows escape from virus neutralization.. J Virol 1993 Apr;67(4):2202-8.
            doi: 10.1128/JVI.67.4.2202-2208.1993pubmed: 8383237google scholar: lookup
          32. Greene WK, Meers J, del Fierro G, Carnegie PR, Robinson WF. Extensive sequence variation of feline immunodeficiency virus env genes in isolates from naturally infected cats.. Arch Virol 1993;133(1-2):51-62.
            doi: 10.1007/BF01309743pubmed: 8240017google scholar: lookup
          33. Martarano L, Stephens R, Rice N, Derse D. Equine infectious anemia virus trans-regulatory protein Rev controls viral mRNA stability, accumulation, and alternative splicing.. J Virol 1994 May;68(5):3102-11.
            doi: 10.1128/JVI.68.5.3102-3111.1994pubmed: 8151775google scholar: lookup
          34. McGuire TC, Tumas DB, Byrne KM, Hines MT, Leib SR, Brassfield AL, O'Rourke KI, Perryman LE. Major histocompatibility complex-restricted CD8+ cytotoxic T lymphocytes from horses with equine infectious anemia virus recognize Env and Gag/PR proteins.. J Virol 1994 Mar;68(3):1459-67.
            doi: 10.1128/JVI.68.3.1459-1467.1994pubmed: 8107209google scholar: lookup
          35. Carey N, Dalziel RG. Sequence variation in the gp135 gene of Maedi visna virus strain EV1.. Virus Genes 1994 Mar;8(2):115-23.
            doi: 10.1007/BF01703610pubmed: 8073632google scholar: lookup
          36. Sellon DC, Fuller FJ, McGuire TC. The immunopathogenesis of equine infectious anemia virus.. Virus Res 1994 May;32(2):111-38.
            doi: 10.1016/0168-1702(94)90038-8pubmed: 8067050google scholar: lookup
          37. Choi WS, Collignon C, Thiriart C, Burns DP, Stott EJ, Kent KA, Desrosiers RC. Effects of natural sequence variation on recognition by monoclonal antibodies neutralize simian immunodeficiency virus infectivity.. J Virol 1994 Sep;68(9):5395-402.
            doi: 10.1128/JVI.68.9.5395-5402.1994pubmed: 7520089google scholar: lookup
          38. Kao WY, Case ST. A novel giant secretion polypeptide in Chironomus salivary glands: implications for another Balbiani ring gene.. J Cell Biol 1985 Sep;101(3):1044-51.
            doi: 10.1083/jcb.101.3.1044pubmed: 4030890google scholar: lookup
          39. Desai SM, Kalyanaraman VS, Casey JM, Srinivasan A, Andersen PR, Devare SG. Molecular cloning and primary nucleotide sequence analysis of a distinct human immunodeficiency virus isolate reveal significant divergence in its genomic sequences.. Proc Natl Acad Sci U S A 1986 Nov;83(21):8380-4.
            doi: 10.1073/pnas.83.21.8380pubmed: 3490666google scholar: lookup
          40. . Immunology of HIV infection and AIDS: memorandum from a WHO/IUIS meeting.. Bull World Health Organ 1987;65(4):453-63.
            pubmed: 3319268
          41. Payne SL, Salinovich O, Nauman SM, Issel CJ, Montelaro RC. Course and extent of variation of equine infectious anemia virus during parallel persistent infections.. J Virol 1987 Apr;61(4):1266-70.
            doi: 10.1128/JVI.61.4.1266-1270.1987pubmed: 3029423google scholar: lookup
          42. Henderson LE, Sowder RC, Smythers GW, Oroszlan S. Chemical and immunological characterizations of equine infectious anemia virus gag-encoded proteins.. J Virol 1987 Apr;61(4):1116-24.
            doi: 10.1128/JVI.61.4.1116-1124.1987pubmed: 3029406google scholar: lookup
          43. Robey WG, Arthur LO, Matthews TJ, Langlois A, Copeland TD, Lerche NW, Oroszlan S, Bolognesi DP, Gilden RV, Fischinger PJ. Prospect for prevention of human immunodeficiency virus infection: purified 120-kDa envelope glycoprotein induces neutralizing antibody.. Proc Natl Acad Sci U S A 1986 Sep;83(18):7023-7.
            doi: 10.1073/pnas.83.18.7023pubmed: 3018753google scholar: lookup
          44. Devare SG, Srinivasan A, Bohan CA, Spira TJ, Curran JW, Kalyanaraman VS. Genomic diversity of the acquired immunodeficiency syndrome retroviruses is reflected in alteration of its translational products.. Proc Natl Acad Sci U S A 1986 Aug;83(15):5718-22.
            doi: 10.1073/pnas.83.15.5718pubmed: 3016717google scholar: lookup
          45. Salinovich O, Payne SL, Montelaro RC, Hussain KA, Issel CJ, Schnorr KL. Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection.. J Virol 1986 Jan;57(1):71-80.
            doi: 10.1128/JVI.57.1.71-80.1986pubmed: 3001367google scholar: lookup
          46. Race RE, Chesebro B, Bloom ME, Aasted B, Wolfinbarger J. Monoclonal antibodies against Aleutian disease virus distinguish virus strains and differentiate sites of virus replication from sites of viral antigen sequestration.. J Virol 1986 Jan;57(1):285-93.
            doi: 10.1128/JVI.57.1.285-293.1986pubmed: 3001352google scholar: lookup
          47. Hahn BH, Gonda MA, Shaw GM, Popovic M, Hoxie JA, Gallo RC, Wong-Staal F. Genomic diversity of the acquired immune deficiency syndrome virus HTLV-III: different viruses exhibit greatest divergence in their envelope genes.. Proc Natl Acad Sci U S A 1985 Jul;82(14):4813-7.
            doi: 10.1073/pnas.82.14.4813pubmed: 2991896google scholar: lookup
          48. Kono Y. Antigenic variation of equine infectious anemia virus as detected by virus neutralization. Brief report.. Arch Virol 1988;98(1-2):91-7.
            doi: 10.1007/BF01321009pubmed: 2829799google scholar: lookup
          49. Le SY, Chen JH, Chatterjee D, Maizel JV. Sequence divergence and open regions of RNA secondary structures in the envelope regions of the 17 human immunodeficiency virus isolates.. Nucleic Acids Res 1989 Apr 25;17(8):3275-88.
            doi: 10.1093/nar/17.8.3275pubmed: 2726458google scholar: lookup
          50. Poss ML, Mullins JI, Hoover EA. Posttranslational modifications distinguish the envelope glycoprotein of the immunodeficiency disease-inducing feline leukemia virus retrovirus.. J Virol 1989 Jan;63(1):189-95.
            doi: 10.1128/JVI.63.1.189-195.1989pubmed: 2535725google scholar: lookup
          51. Gerencer M, Valpotić I, Jukić B, Tomasković M, Basić I. Qualitative analyses of cellular immune functions in equine infectious anemia show homology with AIDS.. Arch Virol 1989;104(3-4):249-57.
            doi: 10.1007/BF01315547pubmed: 2523215google scholar: lookup
          52. Carpenter S, Chesebro B. Change in host cell tropism associated with in vitro replication of equine infectious anemia virus.. J Virol 1989 Jun;63(6):2492-6.
            doi: 10.1128/JVI.63.6.2492-2496.1989pubmed: 2470916google scholar: lookup
          53. McGuire TC, Norton LK, O'Rourke KI, Cheevers WP. Antigenic variation of neutralization-sensitive epitopes of caprine arthritis-encephalitis lentivirus during persistent arthritis.. J Virol 1988 Sep;62(9):3488-92.
            doi: 10.1128/JVI.62.9.3488-3492.1988pubmed: 2457116google scholar: lookup
          54. Hussain KA, Issel CJ, Schnorr KL, Rwambo PM, West M, Montelaro RC. Antigenic mapping of the envelope proteins of equine infectious anemia virus: identification of a neutralization domain and a conserved region on glycoprotein 90.. Arch Virol 1988;98(3-4):213-24.
            doi: 10.1007/BF01322170pubmed: 2450529google scholar: lookup
          55. Carpenter S, Evans LH, Sevoian M, Chesebro B. Role of the host immune response in selection of equine infectious anemia virus variants.. J Virol 1987 Dec;61(12):3783-9.
            doi: 10.1128/JVI.61.12.3783-3789.1987pubmed: 2446008google scholar: lookup
          56. Hussain KA, Issel CJ, Schnorr KL, Rwambo PM, Montelaro RC. Antigenic analysis of equine infectious anemia virus (EIAV) variants by using monoclonal antibodies: epitopes of glycoprotein gp90 of EIAV stimulate neutralizing antibodies.. J Virol 1987 Oct;61(10):2956-61.
            doi: 10.1128/JVI.61.10.2956-2961.1987pubmed: 2442410google scholar: lookup
          57. Bolton V, Pedersen NC, Higgins J, Jennings M, Carlson J. Unique p24 epitope marker to identify multiple human immunodeficiency virus variants in blood from the same individuals.. J Clin Microbiol 1987 Aug;25(8):1411-5.
            doi: 10.1128/jcm.25.8.1411-1415.1987pubmed: 2442190google scholar: lookup
          58. Stanley J, Bhaduri LM, Narayan O, Clements JE. Topographical rearrangements of visna virus envelope glycoprotein during antigenic drift.. J Virol 1987 Apr;61(4):1019-28.
            doi: 10.1128/JVI.61.4.1019-1028.1987pubmed: 2434662google scholar: lookup
          59. Modrow S, Hahn BH, Shaw GM, Gallo RC, Wong-Staal F, Wolf H. Computer-assisted analysis of envelope protein sequences of seven human immunodeficiency virus isolates: prediction of antigenic epitopes in conserved and variable regions.. J Virol 1987 Feb;61(2):570-8.
            doi: 10.1128/JVI.61.2.570-578.1987pubmed: 2433466google scholar: lookup
          60. Kamps MP, Sefton BM. Neither arginine nor histidine can carry out the function of lysine-295 in the ATP-binding site of p60src.. Mol Cell Biol 1986 Mar;6(3):751-7.
            doi: 10.1128/mcb.6.3.751-757.1986pubmed: 2430174google scholar: lookup
          61. Wilson C, Reitz MS Jr, Aldrich K, Klasse PJ, Blomberg J, Gallo RC, Robert-Guroff M. The site of an immune-selected point mutation in the transmembrane protein of human immunodeficiency virus type 1 does not constitute the neutralization epitope.. J Virol 1990 Jul;64(7):3240-8.
            doi: 10.1128/JVI.64.7.3240-3248.1990pubmed: 2352323google scholar: lookup
          62. Poss ML, Quackenbush SL, Mullins JI, Hoover EA. Characterization and significance of delayed processing of the feline leukemia virus FeLV-FAIDS envelope glycoprotein.. J Virol 1990 Sep;64(9):4338-45.
            doi: 10.1128/JVI.64.9.4338-4345.1990pubmed: 2166820google scholar: lookup
          63. Rwambo PM, Issel CJ, Adams WV Jr, Hussain KA, Miller M, Montelaro RC. Equine infectious anemia virus (EIAV) humoral responses of recipient ponies and antigenic variation during persistent infection.. Arch Virol 1990;111(3-4):199-212.
            doi: 10.1007/BF01311054pubmed: 2162160google scholar: lookup
          64. Coppola MA, Thomas CY. A host gene regulates the structure of the transmembrane envelope protein of murine leukemia viruses.. J Exp Med 1990 May 1;171(5):1739-52.
            doi: 10.1084/jem.171.5.1739pubmed: 2159051google scholar: lookup
          65. Burns DP, Desrosiers RC. Selection of genetic variants of simian immunodeficiency virus in persistently infected rhesus monkeys.. J Virol 1991 Apr;65(4):1843-54.
            doi: 10.1128/JVI.65.4.1843-1854.1991pubmed: 2002545google scholar: lookup
          66. Le Grice SF, Panin M, Kalayjian RC, Richter NJ, Keith G, Darlix JL, Payne SL. Purification and characterization of recombinant equine infectious anemia virus reverse transcriptase.. J Virol 1991 Dec;65(12):7004-7.
            doi: 10.1128/JVI.65.12.7004-7007.1991pubmed: 1719238google scholar: lookup
          67. Clabough DL, Gebhard D, Flaherty MT, Whetter LE, Perry ST, Coggins L, Fuller FJ. Immune-mediated thrombocytopenia in horses infected with equine infectious anemia virus.. J Virol 1991 Nov;65(11):6242-51.
            doi: 10.1128/JVI.65.11.6242-6251.1991pubmed: 1717720google scholar: lookup
          68. Kodama T, Burns DP, Silva DP, Veronese FD, Desrosiers RC. Strain-specific neutralizing determinant in the transmembrane protein of simian immunodeficiency virus.. J Virol 1991 Apr;65(4):2010-8.
            doi: 10.1128/JVI.65.4.2010-2018.1991pubmed: 1705994google scholar: lookup
          69. Perryman LE, O'Rourke KI, Mason PH, McGuire TC. Equine monoclonal antibodies recognize common epitopes on variants of equine infectious anaemia virus.. Immunology 1990 Dec;71(4):592-4.
            pubmed: 1703988
          70. Pincus SH, Wehrly K, Tschachler E, Hayes SF, Buller RS, Reitz M. Variants selected by treatment of human immunodeficiency virus-infected cells with an immunotoxin.. J Exp Med 1990 Sep 1;172(3):745-57.
            doi: 10.1084/jem.172.3.745pubmed: 1696955google scholar: lookup
          71. Rwambo PM, Issel CJ, Hussain KA, Montelaro RC. In vitro isolation of a neutralization escape mutant of equine infectious anemia virus (EIAV).. Arch Virol 1990;111(3-4):275-80.
            doi: 10.1007/BF01311062pubmed: 1693846google scholar: lookup
          72. Sellon DC, Perry ST, Coggins L, Fuller FJ. Wild-type equine infectious anemia virus replicates in vivo predominantly in tissue macrophages, not in peripheral blood monocytes.. J Virol 1992 Oct;66(10):5906-13.
            doi: 10.1128/JVI.66.10.5906-5913.1992pubmed: 1382143google scholar: lookup
          73. Ball JM, Rushlow KE, Issel CJ, Montelaro RC. Detailed mapping of the antigenicity of the surface unit glycoprotein of equine infectious anemia virus by using synthetic peptide strategies.. J Virol 1992 Feb;66(2):732-42.
            doi: 10.1128/JVI.66.2.732-742.1992pubmed: 1370556google scholar: lookup
          74. Issel CJ, Horohov DW, Lea DF, Adams WV Jr, Hagius SD, McManus JM, Allison AC, Montelaro RC. Efficacy of inactivated whole-virus and subunit vaccines in preventing infection and disease caused by equine infectious anemia virus.. J Virol 1992 Jun;66(6):3398-408.
            doi: 10.1128/JVI.66.6.3398-3408.1992pubmed: 1316455google scholar: lookup
          75. Weiner AJ, Geysen HM, Christopherson C, Hall JE, Mason TJ, Saracco G, Bonino F, Crawford K, Marion CD, Crawford KA. Evidence for immune selection of hepatitis C virus (HCV) putative envelope glycoprotein variants: potential role in chronic HCV infections.. Proc Natl Acad Sci U S A 1992 Apr 15;89(8):3468-72.
            doi: 10.1073/pnas.89.8.3468pubmed: 1314389google scholar: lookup
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