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Home / Equine Research Bank / Front Vet Sci / Antimicrobial Effects of Equine Platelet Lysate.
Frontiers in veterinary science2021; 8; 703414; doi: 10.3389/fvets.2021.703414

Antimicrobial Effects of Equine Platelet Lysate.

Abstract: The development of antimicrobial resistant bacteria and the lack of novel antibiotic strategies to combat those bacteria is an ever-present problem in both veterinary and human medicine. The goal of this study is to evaluate platelet lysate (PL) as a biological alternative antimicrobial product. Platelet lysate is an acellular platelet-derived product rich in growth factors and cytokines that is manufactured via plateletpheresis and pooled from donor horses. In the current study, we sought to define the antimicrobial properties of PL on select gram-positive and gram-negative bacteria. Results from an end-point assay showed that PL did not support bacterial growth, and in fact significantly reduced bacterial content compared to normal growth media. An assay was then utilized to further determine the effects on bacterial growth dynamics and showed that all strains exhibited a slower growth rate and lower yield in the presence of PL. The specific effects of PL were unique for each bacterial strain: and growth was affected in a concentration-dependent manner, such that higher amounts of PL had a greater effect, while this was not true for or . Furthermore, the onset of exponential growth was delayed for and in the presence of PL, which has significant clinical implications for developing a dosing schedule. In conclusion, our findings demonstrate the potential value of PL as a broad-spectrum antimicrobial that would offer an alternative to traditional antibiotics for the treatment of bacterial infection in equine species.
Copyright © 2021 Gordon, Álvarez-Narváez and Peroni.
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Publication Date: 2021-08-19 PubMed ID: 34490395PubMed Central: PMC8416987DOI: 10.3389/fvets.2021.703414Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study looks at the potential antimicrobial properties of platelet lysate (PL), a product derived from horse platelets, as an alternative to traditional antibiotics in treating bacterial infections in horses. It emerged that PL did not support bacterial growth and even significantly reduced bacterial content.

Research Context

  • The research was conducted as a response to the growing problem of antimicrobial resistant bacteria and the lack of innovative antibiotic strategies in both veterinary and human medicine.
  • It was directed towards finding a biological alternative antimicrobial product, with a special focus on platelet lysate (PL).

Methodology

  • Platelet lysate is an acellular product, rich in growth factors and cytokines obtained from donor horses using plateletpheresis.
  • The research sought to define the antimicrobial properties of PL on selected gram-positive and gram-negative bacteria.
  • An end-point assay was conducted to see how well bacterial growth was supported by the PL. The results from this assay showed that PL did not support and even significantly reduced bacterial growth compared to normal growth media.
  • The team also utilized an assay to determine the effects on bacterial growth dynamics where it emerged that all strains exhibited a slower growth rate and lower yield when exposed to PL.

Key Findings

  • Distinct effects of PL were observed specific to each bacterial strain. The growth of certain bacterial strains was affected in a concentration-dependent manner, implying that higher amounts of PL had a greater effect on these bacteria.
  • For some bacteria, this trend did not hold true and, more interestingly, the onset of exponential growth was delayed for some bacterial strains in the presence of PL.
  • This has significant clinical implications for developing a dosing schedule while using PL as a potential antimicrobial product.

Conclusion

  • The study concludes with a demonstration of the potential value of PL as a broad-spectrum antimicrobial, likely offering a viable and effective alternative to traditional antibiotics for the treatment of bacterial infection in equine species.

Cite This Article

APA
Gordon J, Álvarez-Narváez S, Peroni JF. (2021). Antimicrobial Effects of Equine Platelet Lysate. Front Vet Sci, 8, 703414. https://doi.org/10.3389/fvets.2021.703414

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 8
Pages: 703414
PII: 703414

Researcher Affiliations

Gordon, Julie
  • Department of Large Animal Medicine, Veterinary Medical Center, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Álvarez-Narváez, Sonsiray
  • Athens Veterinary Diagnostic Laboratory, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Peroni, John F
  • Department of Large Animal Medicine, Veterinary Medical Center, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Citations

This article has been cited 4 times.
  1. Tyrnenopoulou P, Fthenakis GC. Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids. Antibiotics (Basel) 2023 Mar 28;12(4).
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  2. Mollabashi M, Klopfer A, Lunardon T, Darzenta N, Davis E, Murray M, Sumner SM, Naskou MC. Plasma and complement proteins are essential for the antimicrobial activity of canine platelet lysate. Front Vet Sci 2025;12:1605649.
    doi: 10.3389/fvets.2025.1605649pubmed: 40666728google scholar: lookup
  3. Egli P, Boone L, Huber L, Higgins C, Gaonkar PP, Arrington J, Naskou MC, Peroni J, Gordon J, Lascola KM. Pilot study characterizing a single pooled preparation of equine platelet lysate for nebulization in the horse. Front Vet Sci 2024;11:1488942.
    doi: 10.3389/fvets.2024.1488942pubmed: 39726585google scholar: lookup
  4. Lunardon T, Sumner SM, Mollabashi M, Darzenta N, Davis E, Naskou MC. Growth factor and cytokine characterization of canine platelet lysate with variable leukocyte concentration, plasma content, and heat-sensitive proteins. Front Vet Sci 2024;11:1408080.
    doi: 10.3389/fvets.2024.1408080pubmed: 39071789google scholar: lookup
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