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Environmental science and pollution research international2018; 25(22); 21789-21800; doi: 10.1007/s11356-018-2274-x

Antimicrobial resistance and the presence of extended-spectrum beta-lactamase genes in Escherichia coli isolated from the environment of horse riding centers.

Abstract: The aim of the study was to determine the antimicrobial resistance profile and the occurrence of extended-spectrum beta-lactamase genes and to analyze the genetic diversity of Escherichia coli strains isolated from the environment of horse riding centers. The study was conducted using E. coli strains isolated from the air, manure, and horse nostril swabs in three horse riding centers differing in the system of horse keeping-stable (OJK Pegaz and KJK Szary) and free-range (SKH Nielepice). Resistance to antibiotics was determined using the disk-diffusion method, and the PCR technique was employed to detect the extended-spectrum β-lactamase (ESBL) genes, while the genetic diversity of strains was assessed by rep-PCR. A total of 200 strains were collected during the 2-year study, with the majority isolated from KJK Szary, while the smallest number was obtained from SKH Nielepice. The strains were mostly resistant to ampicillin, aztreonam, and ticarcillin. The tested strains were most frequently resistant to one or two antibiotics, with a maximum of ten antimicrobials at the same time. Two multidrug-resistant (MDR) strains were detected in OJK Pegaz while in KJK Szary there were two MDR and one extensively drug-resistant (XDR) strain. The ESBL mechanism was most frequently observed in OJK Pegaz (20.31% of strains) followed by KJK Szary (15.53% of strains) and SKH Nielepice (15.15% of strains). Among the ESBL-determining genes, only blaTEM and blaCTXM-9 were detected-blaTEM was mostly found in KJK Szary (53.40% of strains), while the second detected gene-blaCTXM-9-was most frequent in SKH Nielepice (6.06% of strains). The rep-PCR genotyping showed high variation among the analyzed strains, whereas its degree differed between the studied facilities, indicating that the type of horse keeping (stable vs. free-range) affects the genetic diversity of the E. coli strains. Having regard to the fact that the tested strains of E. coli were derived from non-hospitalized horses that were not treated pharmacologically, we can assume that the observed antimicrobial resistance may be of both-natural origin, i.e., not the result of the selection pressure, and acquired, the source of which could be people present in the horse riding facilities, the remaining horses which were not included in the study, and air, as well as water, fodder, and litter of the animals. Therefore, it can be concluded that the studied horses are the source of resistant E. coli and it is reasonable to continue monitoring the changes in antimicrobial resistance in those bacteria.
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Publication Date: 2018-05-23 PubMed ID: 29796881PubMed Central: PMC6063325DOI: 10.1007/s11356-018-2274-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This article discusses a study aimed at determining the antimicrobial resistance and genetic diversity of E. coli strains isolated from various environments in horse riding centers.

Objective of the Study

  • The study aimed to determine the antimicrobial resistance and the occurrence of extended-spectrum beta-lactamase (ESBL) genes in E. coli strains found in various environments at three different horse riding centers.
  • The aim was also to analyze the genetic diversity of the E. coli strains based on the methods of horse keeping employed at the horse riding centers.

Methodology

  • E. coli strains were isolated from air, manure, and nostril swabs from horses at three different horse riding centers. These centers used different methods of horse keeping, namely stable and free-range methods.
  • The antibiotics resistance was determined using the disk-diffusion method. The PCR technique was used to detect the presence of ESBL genes, and the diversity of strains was assessed through rep-PCR.

Findings

  • A majority of the 200 strains collected during the 2-year study were isolated from one particular center, whilst the smallest number was obtained from a free-range horse-keeping center.
  • The strains were mostly resistant to ampicillin, aztreonam, and ticarcillin. The tested strains frequently showed resistance to one or two antibiotics, with a maximum of ten antimicrobials at once.
  • Multi-Drug Resistant (MDR) and Extensively Drug-Resistant (XDR) strains were found predominantly in the stable-keeping centers.
  • The ESBL genes present in the strains varied with the type of horse-keeping method.

Implications and Conclusions

  • Genotyping showed considerable variation among the analysed strains, suggesting that the type of horse keeping affects the genetic diversity of E. coli strains.
  • The tested E. coli strains were derived from non-hospitalized horses. Therefore, it is plausible that the observed antimicrobial resistance could be of natural origin and not a result of selection pressure.
  • It is concluded that horses could be a source of resistant E. coli and thus, it is necessary to continue monitoring changes in antimicrobial resistance in these bacteria.

Cite This Article

APA
Wolny-Koładka K, Lenart-Boroń A. (2018). Antimicrobial resistance and the presence of extended-spectrum beta-lactamase genes in Escherichia coli isolated from the environment of horse riding centers. Environ Sci Pollut Res Int, 25(22), 21789-21800. https://doi.org/10.1007/s11356-018-2274-x

Publication

Environmental science and pollution research international
ISSN: 1614-7499
NlmUniqueID: 9441769
Country: Germany
Language: English
Volume: 25
Issue: 22
Pages: 21789-21800

Researcher Affiliations

Wolny-Koładka, Katarzyna
  • Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059, Cracow, Poland. katarzyna.wolny@urk.edu.pl.
Lenart-Boroń, Anna
  • Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059, Cracow, Poland.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Bacterial / drug effects
  • Drug Resistance, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial / drug effects
  • Drug Resistance, Multiple, Bacterial / genetics
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli Proteins / genetics
  • Genetic Variation
  • Horses / microbiology
  • Manure / microbiology
  • Poland
  • beta-Lactamases / genetics

References

This article includes 50 references
  1. Ahmed MO, Clegg PD, Williams NJ, Baptiste KE, Bennett M. Antimicrobial resistance in equine faecal Escherichia coli isolates from North West England.. Ann Clin Microbiol Antimicrob 2010 Apr 7;9:12.
    doi: 10.1186/1476-0711-9-12pmc: PMC2867969pubmed: 20374640google scholar: lookup
  2. Alekshun MN, Levy SB. Molecular mechanisms of antibacterial multidrug resistance.. Cell 2007 Mar 23;128(6):1037-50.
    doi: 10.1016/j.cell.2007.03.004pubmed: 17382878google scholar: lookup
  3. Angulo FJ, Nunnery JA, Bair HD. Antimicrobial resistance in zoonotic enteric pathogens.. Rev Sci Tech 2004 Aug;23(2):485-96.
    doi: 10.20506/rst.23.2.1499pubmed: 15702715google scholar: lookup
  4. Baraniak A. Molecular epidemiology and evolution of Enterobacteriaceae strains producing extended spectrum blactamases (ESBL) in Poland. 2010.
  5. Barry AL, Jones RN, Thornsberry C. Evaluation of the cefonicid disk test criteria, including disk quality control guidelines.. J Clin Microbiol 1983 Feb;17(2):232-9.
    pmc: PMC272613pubmed: 6601113doi: 10.1128/jcm.17.2.232-239.1983google scholar: lookup
  6. Böhme K, Fernández-No IC, Barros-Velázquez J, Gallardo JM, Calo-Mata P, Cañas B. Species differentiation of seafood spoilage and pathogenic gram-negative bacteria by MALDI-TOF mass fingerprinting.. J Proteome Res 2010 Jun 4;9(6):3169-83.
    doi: 10.1021/pr100047qpubmed: 20408567google scholar: lookup
  7. Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.. Clin Microbiol Rev 2001 Oct;14(4):933-51, table of contents.
    doi: 10.1128/CMR.14.4.933-951.2001pmc: PMC89009pubmed: 11585791google scholar: lookup
  8. Briñas L, Zarazaga M, Sáenz Y, Ruiz-Larrea F, Torres C. Beta-lactamases in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals.. Antimicrob Agents Chemother 2002 Oct;46(10):3156-63.
    doi: 10.1128/AAC.46.10.3156-3163.2002pmc: PMC128764pubmed: 12234838google scholar: lookup
  9. Bryan J, Leonard N, Fanning S, Katz L, Duggan V. Antimicrobial resistance in commensal faecal Escherichia coli of hospitalised horses.. Ir Vet J 2010 Jun 1;63(6):373-9.
    doi: 10.1186/2046-0481-63-6-373pmc: PMC3113860pubmed: 21851747google scholar: lookup
  10. Commission Regulation (EC) No 504/2008 of 6 June 2008 implementing Council Directives 90/426/EEC and 90/427/EEC as regards methods for the identification of equidae (in Polish)
  11. Costa D, Poeta P, Sáenz Y, Vinué L, Rojo-Bezares B, Jouini A, Zarazaga M, Rodrigues J, Torres C. Detection of Escherichia coli harbouring extended-spectrum beta-lactamases of the CTX-M, TEM and SHV classes in faecal samples of wild animals in Portugal.. J Antimicrob Chemother 2006 Dec;58(6):1311-2.
    doi: 10.1093/jac/dkl415pubmed: 17023496google scholar: lookup
  12. Croxatto A, Prod'hom G, Greub G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.. FEMS Microbiol Rev 2012 Mar;36(2):380-407.
    doi: 10.1111/j.1574-6976.2011.00298.xpubmed: 22092265google scholar: lookup
  13. Dolejska M, Duskova E, Rybarikova J, Janoszowska D, Roubalova E, Dibdakova K, Maceckova G, Kohoutova L, Literak I, Smola J, Cizek A. Plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre.. J Antimicrob Chemother 2011 Apr;66(4):757-64.
    doi: 10.1093/jac/dkq500pubmed: 21393204google scholar: lookup
  14. Drieux L, Brossier F, Sougakoff W, Jarlier V. Phenotypic detection of extended-spectrum beta-lactamase production in Enterobacteriaceae: review and bench guide.. Clin Microbiol Infect 2008 Jan;14 Suppl 1:90-103.
    doi: 10.1111/j.1469-0691.2007.01846.xpubmed: 18154532google scholar: lookup
  15. Dunowska M, Morley PS, Traub-Dargatz JL, Hyatt DR, Dargatz DA. Impact of hospitalization and antimicrobial drug administration on antimicrobial susceptibility patterns of commensal Escherichia coli isolated from the feces of horses.. J Am Vet Med Assoc 2006 Jun 15;228(12):1909-17.
    doi: 10.2460/javma.228.12.1909pubmed: 16784384google scholar: lookup
  16. European Committee on Antimicrobial Susceptibility Testing. Clinical breakpoints – bacteria (v. 7.0). 2017.
  17. Excoffier L, Lischer HE. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows.. Mol Ecol Resour 2010 May;10(3):564-7.
    doi: 10.1111/j.1755-0998.2010.02847.xpubmed: 21565059google scholar: lookup
  18. Gniadkowski M, Żabicka D, Hryniewicz W. Recommended selection of the antimicrobial susceptibility tests for bacteria. Determination of Gram-negative rods susceptibility. 2009.
  19. Hanberger H, Arman D, Gill H, Jindrák V, Kalenic S, Kurcz A, Licker M, Naaber P, Scicluna EA, Vanis V, Walther SM. Surveillance of microbial resistance in European Intensive Care Units: a first report from the Care-ICU programme for improved infection control.. Intensive Care Med 2009 Jan;35(1):91-100.
    doi: 10.1007/s00134-008-1237-ypubmed: 18670757google scholar: lookup
  20. Heuer H, Schmitt H, Smalla K. Antibiotic resistance gene spread due to manure application on agricultural fields.. Curr Opin Microbiol 2011 Jun;14(3):236-43.
    doi: 10.1016/j.mib.2011.04.009pubmed: 21546307google scholar: lookup
  21. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies.. Mol Biol Evol 2006 Feb;23(2):254-67.
    doi: 10.1093/molbev/msj030pubmed: 16221896google scholar: lookup
  22. Kadlec K, Schwarz S. Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study.. J Antimicrob Chemother 2008 Sep;62(3):469-73.
    doi: 10.1093/jac/dkn233pubmed: 18550679google scholar: lookup
  23. Kosikowska U, Stępień-Pyśniak D, Pietras-Ożga D, Andrzejczuk J, Juda M, Malm A. Application of MALDI-TOF MS for identification of clinical isolates of bacteria from humans and animals. Diagn Lab 2015;51(1):23–30.
  24. Kronvall G, Petersson AC, Ljunggren K, Soltesz V. Single-strain regression analysis for quality control of cephalothin-susceptibility testing and determination of interpretive breakpoints.. Acta Pathol Microbiol Immunol Scand B 1984 Feb;92(1):13-22.
    pubmed: 6369872doi: 10.1111/j.1699-0463.1984.tb02788.xgoogle scholar: lookup
  25. Lenart-Boroń A. Antimicrobial resistance and prevalence of extended-spectrum beta-lactamase genes in Escherichia coli from major rivers in Podhale, southern Poland. Int J Environ Sci Technol 2017;14:241–250.
    doi: 10.1007/s13762-016-1155-4google scholar: lookup
  26. Lenart-Boroń A, Augustyniak K, Boroń P. Screening of antimicrobial resistance and molecular detection of fluoroquinolone resistance mechanisms in chicken faeces-derived Escherichia coli. Vet Med 2016;61(2):80–89.
    doi: 10.17221/8721-VETMEDgoogle scholar: lookup
  27. Lenart-Boroń A, Wolny-Koładka K, Juraszek K, Kasprowicz A. Phenotypic and molecular assessment of antimicrobial resistance profile of airborne Staphylococcus spp. isolated from flats in Kraków.. Aerobiologia (Bologna) 2017;33(3):435-444.
    doi: 10.1007/s10453-017-9481-7pmc: PMC5591801pubmed: 28955110google scholar: lookup
  28. Maddox TW, Clegg PD, Diggle PJ, Wedley AL, Dawson S, Pinchbeck GL, Williams NJ. Cross-sectional study of antimicrobial-resistant bacteria in horses. Part 1: Prevalence of antimicrobial-resistant Escherichia coli and methicillin-resistant Staphylococcus aureus.. Equine Vet J 2012 May;44(3):289-96.
    doi: 10.1111/j.2042-3306.2011.00441.xpubmed: 21848534google scholar: lookup
  29. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance.. Clin Microbiol Infect 2012 Mar;18(3):268-81.
    doi: 10.1111/j.1469-0691.2011.03570.xpubmed: 21793988google scholar: lookup
  30. Marcadé G, Deschamps C, Boyd A, Gautier V, Picard B, Branger C, Denamur E, Arlet G. Replicon typing of plasmids in Escherichia coli producing extended-spectrum beta-lactamases.. J Antimicrob Chemother 2009 Jan;63(1):67-71.
    doi: 10.1093/jac/dkn428pubmed: 18931389google scholar: lookup
  31. Nunnery J, Angulo FJ, Tollefson L. Public health and policy.. Prev Vet Med 2006 Feb 24;73(2-3):191-5.
    doi: 10.1016/j.prevetmed.2005.09.014pubmed: 16269192google scholar: lookup
  32. . Operator’s Manual MAS-100TM professional Microbial Air Monitoring System for the Microbiological Testing of Air. .
  33. Picozzi SC, Casellato S, Rossini M, Paola G, Tejada M, Costa E, Carmignani L. Extended-spectrum beta-lactamase-positive Escherichia coli causing complicated upper urinary tract infection: Urologist should act in time.. Urol Ann 2014 Apr;6(2):107-12.
    doi: 10.4103/0974-7796.130536pmc: PMC4021646pubmed: 24833818google scholar: lookup
  34. Rawat D, Nair D. Extended-spectrum β-lactamases in Gram Negative Bacteria.. J Glob Infect Dis 2010 Sep;2(3):263-74.
    doi: 10.4103/0974-777X.68531pmc: PMC2946684pubmed: 20927289google scholar: lookup
  35. Sader HS, Ferraro MJ, Reller LB, Schreckenberger PC, Swenson JM, Jones RN. Reevaluation of Clinical and Laboratory Standards Institute disk diffusion breakpoints for tetracyclines for testing Enterobacteriaceae.. J Clin Microbiol 2007 May;45(5):1640-3.
    doi: 10.1128/JCM.00143-07pmc: PMC1865907pubmed: 17360844google scholar: lookup
  36. Sáenz Y, Briñas L, Domínguez E, Ruiz J, Zarazaga M, Vila J, Torres C. Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains of human, animal, and food origins.. Antimicrob Agents Chemother 2004 Oct;48(10):3996-4001.
    doi: 10.1128/AAC.48.10.3996-4001.2004pmc: PMC521888pubmed: 15388464google scholar: lookup
  37. Scott Weese J. Antimicrobial resistance in companion animals.. Anim Health Res Rev 2008 Dec;9(2):169-76.
    doi: 10.1017/S1466252308001485pubmed: 18983722google scholar: lookup
  38. Seng P, Rolain JM, Fournier PE, La Scola B, Drancourt M, Raoult D. MALDI-TOF-mass spectrometry applications in clinical microbiology.. Future Microbiol 2010 Nov;5(11):1733-54.
    doi: 10.2217/fmb.10.127pubmed: 21133692google scholar: lookup
  39. Simarro E, Navarro F, Ruiz J, Miró E, Gómez J, Mirelis B. Salmonella enterica serovar virchow with CTX-M-like beta-lactamase in Spain.. J Clin Microbiol 2000 Dec;38(12):4676-8.
    pmc: PMC87664pubmed: 11101623doi: 10.1128/jcm.38.12.4676-4678.2000google scholar: lookup
  40. Turnidge JD. Cefazolin and enterobacteriaceae: rationale for revised susceptibility testing breakpoints.. Clin Infect Dis 2011 Apr 1;52(7):917-24.
    doi: 10.1093/cid/cir031pubmed: 21427400google scholar: lookup
  41. Venglovsky J, Sasakova N, Placha I. Pathogens and antibiotic residues in animal manures and hygienic and ecological risks related to subsequent land application.. Bioresour Technol 2009 Nov;100(22):5386-91.
    doi: 10.1016/j.biortech.2009.03.068pubmed: 19386485google scholar: lookup
  42. Versalovic J, Schneider M, De Bruin FJ, Lupski JR. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Method Mol Cell Biol 1994;5:25–40.
  43. Villesen P. FaBox: an online toolbox for fasta sequences. Mol Ecol Notes 2007;7:965–968.
    doi: 10.1111/j.1471-8286.2007.01821.xgoogle scholar: lookup
  44. Vo AT, van Duijkeren E, Fluit AC, Gaastra W. Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses.. Vet Microbiol 2007 Oct 6;124(3-4):248-55.
    doi: 10.1016/j.vetmic.2007.04.027pubmed: 17521833google scholar: lookup
  45. Waran N. Welfare of horses. 2002;pp. 79–80.
  46. Wellington EM, Boxall AB, Cross P, Feil EJ, Gaze WH, Hawkey PM, Johnson-Rollings AS, Jones DL, Lee NM, Otten W, Thomas CM, Williams AP. The role of the natural environment in the emergence of antibiotic resistance in gram-negative bacteria.. Lancet Infect Dis 2013 Feb;13(2):155-65.
    doi: 10.1016/S1473-3099(12)70317-1pubmed: 23347633google scholar: lookup
  47. Wolny-Koładka K, Lenart-Boroń A. Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli.. Water Air Soil Pollut 2016;227:146.
    doi: 10.1007/s11270-016-2833-zpmc: PMC4835524pubmed: 27158170google scholar: lookup
  48. Wolny-Koładka K, Lenart-Boroń A, Kasprowicz A. Disc-diffusion and PCR detection of methicillin resistance in environmental airborne strains of Staphylococcus spp.. Pol J Microbiol 2014;63(3):363-8.
    pubmed: 25546949
  49. Wolny-Koładka K, Malina D. Toxicity assessment of silver nanoparticles against Escherichia coli strains isolated from horse dung. Micro Nano Lett 2017;12:772–776.
    doi: 10.1049/mnl.2017.0129google scholar: lookup
  50. Wróblewski Z, Wojtaszek A. Principles of good veterinary practice in horses therapy. Życie Weterynaryjne 2015;90(5):298–301.

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  1. Anyanwu MU, Jaja IF, Nwobi OC, Mgbeahuruike AC, Ikpendu CN, Okafor NA, Oguttu JW. Epidemiology and Traits of Mobile Colistin Resistance (mcr) Gene-Bearing Organisms from Horses. Microorganisms 2022 Jul 25;10(8).
    doi: 10.3390/microorganisms10081499pubmed: 35893557google scholar: lookup
  2. Thomson K, Eskola K, Eklund M, Suominen K, Määttä M, Junnila J, Nykäsenoja S, Niinistö K, Grönthal T, Rantala M. Characterisation of and risk factors for extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) in an equine hospital with a special reference to an outbreak caused by Klebsiella pneumoniae ST307:CTX-M-1. Acta Vet Scand 2022 Feb 9;64(1):4.
    doi: 10.1186/s13028-022-00621-6pubmed: 35139865google scholar: lookup
  3. Bolukaoto JY, Singh A, Alfinete N, Barnard TG. Occurrence of Hybrid Diarrhoeagenic Escherichia coli Associated with Multidrug Resistance in Environmental Water, Johannesburg, South Africa. Microorganisms 2021 Oct 17;9(10).
    doi: 10.3390/microorganisms9102163pubmed: 34683484google scholar: lookup
  4. Formenti N, Calò S, Parisio G, Guarneri F, Birbes L, Pitozzi A, Scali F, Tonni M, Guadagno F, Giovannini S, Salogni C, Ianieri A, Bellini S, Pasquali P, Alborali GL. ESBL/AmpC-Producing Escherichia coli in Wild Boar: Epidemiology and Risk Factors. Animals (Basel) 2021 Jun 22;11(7).
    doi: 10.3390/ani11071855pubmed: 34206498google scholar: lookup
  5. Shnaiderman-Torban A, Navon-Venezia S, Paitan Y, Archer H, Abu Ahmad W, Bonder D, Hanael E, Nissan I, Zizelski Valenci G, Weese SJ, Steinman A. Extended spectrum β lactamase-producing Enterobacteriaceae shedding by race horses in Ontario, Canada. BMC Vet Res 2020 Dec 9;16(1):479.
    doi: 10.1186/s12917-020-02701-zpubmed: 33298039google scholar: lookup
  6. Sukmawinata E, Uemura R, Sato W, Mitoma S, Kanda T, Sueyoshi M. IncI1 Plasmid Associated with bla(CTX-M-2) Transmission in ESBL-Producing Escherichia coli Isolated from Healthy Thoroughbred Racehorse, Japan. Antibiotics (Basel) 2020 Feb 7;9(2).
    doi: 10.3390/antibiotics9020070pubmed: 32046117google scholar: lookup
  7. Hordijk J, Farmakioti E, Smit LAM, Duim B, Graveland H, Theelen MJP, Wagenaar JA. Fecal Carriage of Extended-Spectrum-β-Lactamase/AmpC-Producing Escherichia coli in Horses. Appl Environ Microbiol 2020 Apr 1;86(8).
    doi: 10.1128/AEM.02590-19pubmed: 32033947google scholar: lookup
  8. Arul Selvaraj RC, Rajendran M, Nagaiah HP. Re-Potentiation of β-Lactam Antibiotic by Synergistic Combination with Biogenic Copper Oxide Nanocubes against Biofilm Forming Multidrug-Resistant Bacteria. Molecules 2019 Aug 22;24(17).
    doi: 10.3390/molecules24173055pubmed: 31443467google scholar: lookup
  9. Khalid E, Tartor YH, Ammar AM, Abdelaziz R, Mahmmod Y, Abdelkhalek A. Controlling drug-resistant bacteria in Arabian horses: bacteriophage cocktails for treating wound infections. Front Vet Sci 2025;12:1609955.
    doi: 10.3389/fvets.2025.1609955pubmed: 41169678google scholar: lookup
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