Antimicrobial resistance of Salmonella isolates from cattle and horses with salmonellosis in Hokkaido, Japan.
Abstract: In recent years, reported cases of salmonellosis in cattle and horses in Hokkaido, Japan, have increased. To optimize antimicrobial therapy, we assessed the antimicrobial susceptibility of Salmonella isolates from these animals. Among 112 bovine isolates, high resistance rates were observed for streptomycin (92.0%), ampicillin (88.4%), and tetracycline (79.5%), while resistance rates for kanamycin (8.9%), ciprofloxacin (1.8%), gentamicin (0.0%), and colistin (0.0%) were low. Additionally, 18.8% of the bovine isolates were resistant to cefotaxime, and all of these cefotaxime-resistant isolates carried bla. Among 16 equine isolates, no resistance was observed to cefazolin, cefotaxime, gentamicin, nalidixic acid, ciprofloxacin, colistin, or chloramphenicol. Gentamicin and kanamycin are recommended as the first-line treatment for salmonellosis in cattle, with fluoroquinolones as the second choice.
Publication Date: 2024-10-16 PubMed ID: 39414453PubMed Central: PMC11612240DOI: 10.1292/jvms.24-0236Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
Overview
- This study evaluated the antimicrobial resistance patterns of Salmonella bacteria isolated from cattle and horses with salmonellosis in Hokkaido, Japan, to inform effective treatment strategies.
Background
- Salmonellosis is a significant infectious disease affecting cattle and horses, causing gastrointestinal illness and economic losses.
- Recent years have seen an increase in reported salmonellosis cases in Hokkaido, Japan.
- Antimicrobial therapy is crucial for managing outbreaks, but rising antimicrobial resistance complicates treatment choices.
- The study aimed to assess susceptibility of Salmonella isolates to various antibiotics to guide optimal treatment regimens.
Methods and Sample
- A total of 112 Salmonella isolates were collected from cattle diagnosed with salmonellosis.
- Additionally, 16 isolates were obtained from horses diagnosed with the disease.
- Antimicrobial susceptibility testing was performed to evaluate resistance to a panel of antibiotics commonly used in veterinary practice.
Findings: Antimicrobial Resistance in Cattle Isolates
- High resistance rates were observed to the following antibiotics:
- Streptomycin: 92.0%
- Ampicillin: 88.4%
- Tetracycline: 79.5%
- Low resistance rates were found for:
- Kanamycin: 8.9%
- Ciprofloxacin: 1.8%
- Gentamicin and colistin: 0.0% (no resistance)
- Notably, 18.8% of isolates were resistant to cefotaxime, a third-generation cephalosporin.
- All cefotaxime-resistant isolates carried the bla gene, which is typically associated with beta-lactamase enzyme production conferring resistance to beta-lactam antibiotics like cephalosporins.
Findings: Antimicrobial Resistance in Horse Isolates
- Among the 16 equine isolates:
- No resistance was detected against cefazolin, cefotaxime, gentamicin, nalidixic acid, ciprofloxacin, colistin, or chloramphenicol.
- This indicates a generally low level of antimicrobial resistance in Salmonella from horses compared to cattle.
Implications for Treatment
- Based on resistance patterns, gentamicin and kanamycin are recommended as the first-choice antimicrobials for treating salmonellosis in cattle in this region.
- Fluoroquinolones (such as ciprofloxacin), having low resistance rates, are considered the second-line treatment option.
- The high resistance levels to commonly used antibiotics like streptomycin, ampicillin, and tetracycline suggest they may be less effective and should be used cautiously.
- Continuous monitoring of antimicrobial susceptibility is essential to adapt treatment protocols over time and to prevent treatment failures.
Conclusion
- This study highlights the concerning high resistance rates among Salmonella isolates from cattle, especially to older antibiotics, emphasizing the need for targeted antimicrobial use.
- Salmonella isolates from horses showed lower resistance levels, indicating different resistance dynamics between animal species.
- Veterinary practitioners in Hokkaido should consider these results when selecting antimicrobial agents for salmonellosis treatment to ensure efficacy and limit the spread of resistance.
Cite This Article
APA
Sasaki Y, Suzuki S, Kusaba N, Rahman N, Aikawa C, Okamura M.
(2024).
Antimicrobial resistance of Salmonella isolates from cattle and horses with salmonellosis in Hokkaido, Japan.
J Vet Med Sci, 86(12), 1227-1232.
https://doi.org/10.1292/jvms.24-0236 Publication
Researcher Affiliations
- Division of Veterinary Science, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
- Division of Veterinary Science, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
- Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
- Division of Veterinary Science, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
- Division of Veterinary Science, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
- Division of Veterinary Science, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
MeSH Terms
- Animals
- Cattle
- Horses
- Japan
- Salmonella Infections, Animal / microbiology
- Salmonella Infections, Animal / epidemiology
- Salmonella Infections, Animal / drug therapy
- Salmonella / drug effects
- Salmonella / isolation & purification
- Anti-Bacterial Agents / pharmacology
- Anti-Bacterial Agents / therapeutic use
- Horse Diseases / microbiology
- Horse Diseases / drug therapy
- Cattle Diseases / microbiology
- Cattle Diseases / drug therapy
- Cattle Diseases / epidemiology
- Drug Resistance, Bacterial
- Microbial Sensitivity Tests
Conflict of Interest Statement
The authors have no conflict of interest.
References
This article includes 21 references
- Arai N, Sekizuka T, Tamamura-Andoh Y, Barco L, Hinenoya A, Yamasaki S, Iwata T, Watanabe-Yanai A, Kuroda M, Akiba M, Kusumoto M. Identification of a recently dominant sublineage in S 4,[5],12:i:- sequence type 34 isolated from food animals in Japan.. 12: 690947.
- Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, 3rd ed., CLSI guideline M45. .
- Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 34th informational supplement. M100-Ed34. .
- Costa LF, Paixão TA, Tsolis RM, Bäumler AJ, Santos RL. Salmonellosis in cattle: advantages of being an experimental model.. 93: 1–6.
- Grandolfo E, Parisi A, Ricci A, Lorusso E, de Siena R, Trotta A, Buonavoglia D, Martella V, Corrente M. High mortality in foals associated with subsp. Abortusequi infection in Italy.. 30: 483–485.
- Holschbach CL, Peek SF. 2018. in dairyncattle. 34: 133–154. doi:n10.1016/j.cvfa.2017.10.005n
- Hong Y, Ji R, Wang Z, Gu J, Jiao X, Li Q. Development and application of a multiplex PCR method to differentiate serovar Typhimurium from its monophasic variants in pig farms.. 109: 104135.
- Kijima M, Shirakawa T, Uchiyama M, Kawanishi M, Ozawa M, Koike R. Trends in the serovar and antimicrobial resistance in clinical isolates of from cattle and pigs between 2002 and 2016 in Japan.. 127: 1869–1875.
- Kumagai Y, Pires SM, Kubota K, Asakura H. Attributing human foodborne diseases to food sources and water in Japan using analysis of outbreak surveillance data.. 83: 2087–2094.
- Lee K, Iwata T, Nakadai A, Kato T, Hayama S, Taniguchi T, Hayashidani H. Prevalence of and spp. in feral raccoons (Procyon lotor) and masked palm civets () in Japan.. 58: 424–431.
- National Veterinary Assay Laboratory of Japan. Report on the Japanese veterinary antimicrobial resistance monitoring system 2016–2017. .
- Niwa H, Anzai T, Izumiya H, Morita-Ishihara T, Watanabe H, Uchida I, Tozaki T, Hobo S. Antimicrobial resistance and genetic characteristics of Typhimurium isolated from horses in Hokkaido, Japan.. 71: 1115–1119.
- Noda T, Murakami K, Etoh Y, Okamoto F, Yatsuyanagi J, Sera N, Furuta M, Onozuka D, Oda T, Asai T, Fujimoto S. Increase in resistance to extended-spectrum cephalosporins in isolated from retail chicken products in Japan.. 10: e0116927.
- . Salmonella and salmonellosis in horses: an overview.. Vet Rec : 659–660.
- Pérez-Pérez FJ, Hanson ND. Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR.. 40: 2153–2162.
- Sarashina T, Ichijo S, Osame T, Seno N, Hosokawa K, Watanabe T, Yoshida S, Akajima A, Sasaki S. Therapeutic effect of gentamicin on diarrhea of calves. 38: 235–238 (in Japanese with English summary).
- Sasaki Y, Murakami M, Haruna M, Maruyama N, Mori T, Ito K, Yamada Y. Prevalence and characterization of foodborne pathogens in dairy cattle in the eastern part of Japan. 75: 543–546.
- Sasaki Y, Ikeda T, Yonemitsu K, Kuroda M, Ogawa M, Sakata R, Uema M, Momose Y, Ohya K, Watanabe M, Hara-Kudo Y, Okamura M, Asai T. Antimicrobial resistance profiles of and spp. isolated from enteritis patients in Japan. 85: 463–470.
- Stepan RM, Sherwood JS, Petermann SR, Logue CM. Molecular and comparative analysis of enterica Senftenberg from humans and animals using PFGE, MLST and NARMS. 11: 153.
- World Health Organization. Critically important antimicrobials for human medicine, 6th revision. .
- Yamaguchi E, Fujii K, Kayano M, Sakurai Y, Nakatani A, Sasaki M, Hertl JA, Grohn YT. Is enterica shared between wildlife and cattle in cattle farming areas? An 11-year retrospective study in Tokachi district, Hokkaido, Japan. 8: 758–770.
Citations
This article has been cited 1 times.- Sasaki Y, Ohya K, Momose Y, Uema M, Ikeda T, Sasaki M, Asai T. Serovars and Antimicrobial Resistance of Salmonella in Food Workers and Livestock Products: Insights into Foodborne Transmission Pathways in Eastern Japan.. Pathogens 2025 Sep 23;14(10).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists