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Home / Equine Research Bank / J Equine Sci / Antimicrobial resistance profiles and phylogenetic groups of...
Journal of equine science2020; 31(4); 85-91; doi: 10.1294/jes.31.85

Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan.

Abstract: In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association training centers. E. coli was isolated by using selective agar media, deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB). A total of 417 E. coli isolates were examined against 10 antimicrobial agents by using the broth microdilution method. The 417 E. coli isolates were phylogenetically grouped using a multiplex polymerase chain reaction. The highest proportion of resistance was observed for streptomycin (30.9%, 129/417) followed by ampicillin (19.4%, 81/417), trimethoprim (15.8%, 66/417), tetracycline (8.4%, 35/417), chloramphenicol (2.6%, 11/417), kanamycin (1.2%, 5/417), nalidixic acid (0.5%, 2/417), cefazolin (0.2%, 1/417), colistin (0.2%, 1/417), and gentamycin (0%). Multidrug-resistant (MDR) E. coli was detected in 7.9% (33/417) of isolates. The proportions of resistance against ampicillin, streptomycin, kanamycin, and chloramphenicol and of multidrug-resistant phenotypes in E. coli belonging to phylogenetic group B2 were significantly higher than those of other groups. This study clarified the distribution of antimicrobial-resistant (AMR) E. coli in Japanese racehorses. A continuous monitoring program for antimicrobial resistance is required to control the spread of AMR bacteria in racehorses.
©2020 The Japanese Society of Equine Science.
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Publication Date: 2020-12-18 PubMed ID: 33376444PubMed Central: PMC7750643DOI: 10.1294/jes.31.85Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study focuses on examining the prevalence of antimicrobial resistance in Escherichia coli, a type of bacteria, collected from healthy thoroughbred racehorses in Japan. It sheds light on the resistance profiles to different antibiotics and their relation to the bacteria’s phylogenetic groups.

Collection and Isolation Method

  • The study was conducted from March 2017 to August 2018. The researchers collected 212 fecal samples from thoroughbred racehorses at Japan Racing Association training centers.
  • They isolated the E. coli from these samples using selective agar media, namely deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB).
  • The process resulted in a total of 417 E. coli isolates.

Antimicrobial Resistance Test

  • All 417 E. coli isolates underwent a test for resistance using the broth microdilution method. This test checked the bacteria’s resistance to 10 antimicrobial agents.
  • The agents tested included streptomycin, ampicillin, trimethoprim, tetracycline, chloramphenicol, kanamycin, nalidixic acid, cefazolin, colistin, and gentamycin.
  • The study found that the E. coli samples showed the highest resistance to streptomycin at 30.9%, followed by ampicillin at 19.4%, and trimethoprim at 15.8%. The least resistance was for gentamycin, for which no resistance was observed.
  • In total, the study detected multidrug-resistant (MDR) E. coli in 7.9% of the isolates.

Phylogenetic Grouping and Resistance

  • After the resistance test, the isolates underwent multiplex polymerase chain reaction to be grouped phylogenetically. This allows the researchers to understand the genetic variations and evolution of the E. coli samples.
  • The study found that the proportions of resistance against certain antibiotics, specifically ampicillin, streptomycin, kanamycin, and chloramphenicol, and of multidrug-resistant phenotypes were significantly higher in E. coli belonging to phylogenetic group B2 when compared to other groups.

Implications

  • This study provides a clearer picture of the presence and spread of antimicrobial-resistant (AMR) E. coli in Japanese racehorses, which may help in managing antimicrobial resistance in animals.
  • The researchers suggest that there is a need for continuous monitoring of antimicrobial resistance to help control the spread of AMR bacteria among racehorses.

Cite This Article

APA
Sato W, Sukmawinata E, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, Sueyoshi M. (2020). Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan. J Equine Sci, 31(4), 85-91. https://doi.org/10.1294/jes.31.85

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 31
Issue: 4
Pages: 85-91

Researcher Affiliations

Sato, Wataru
  • Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Sukmawinata, Eddy
  • Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Uemura, Ryoko
  • Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
  • Center for Animal Diseases Control, University of Miyazaki, Miyazaki 889-2192, Japan.
Kanda, Takuya
  • Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Kusano, Kanichi
  • Miho Training Center, Racehorse Clinic, Japan Racing Association, Ibaraki 300-0400, Japan.
Kambayashi, Yoshinori
  • Miho Training Center, Racehorse Clinic, Japan Racing Association, Ibaraki 300-0400, Japan.
Sato, Takashi
  • Ritto Training Center, Racehorse Clinic, Japan Racing Association, Shiga 520-3005, Japan.
Ishikawa, Yuhiro
  • Ritto Training Center, Racehorse Clinic, Japan Racing Association, Shiga 520-3005, Japan.
Toya, Ryohei
  • Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Sueyoshi, Masuo
  • Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
  • Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.

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Citations

This article has been cited 1 times.
  1. Wongtawan T, Narinthorn R, Sontigun N, Sansamur C, Petcharat Y, Fungwithaya P, Saengsawang P, Blackall PJ, Thomrongsuwannakij T. Characterizing the antimicrobial resistance profile of Escherichia coli found in sport animals (fighting cocks, fighting bulls, and sport horses) and soils from their environment.. Vet World 2022 Nov;15(11):2673-2680.
    doi: 10.14202/vetworld.2022.2673-2680pubmed: 36590125google scholar: lookup
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