Antioxidant capacity and athletic condition of endurance horses undergoing nutraceutical supplementation.
Abstract: Endurance is an equestrian discipline that primarily relies on aerobic metabolism. Intense aerobic exercise produces reactive oxygen species due to an imbalance between oxidant and antioxidant substances, known as oxidative stress, which may reduce athletic performance. This study evaluated the effects of a feed supplement containing natural antioxidants and omega-3 fatty acids on the blood antioxidant activity and the athletic condition of endurance horses undergoing an exercise test. Twelve Arabian endurance horses were randomly assigned to treatment or control groups. At T0, blood lactate, whole blood and red blood cells (RBC) antioxidant capacity were assessed. The horses performed an exercise test with heart rate monitoring. After 30 minutes, blood lactate, antioxidant capacity and serum creatine kinase (CK) were measured. The treatment group received the dietary supplement for 21 days, while controls maintained their diet. Then, the protocol was repeated (T1). Variables were compared within and between groups through two-way ANOVA and post-hoc tests. Significant time*group effects were observed for serum CK (p = 0.026), RBC antioxidant capacity at rest (p = 0.034) and post-exercise (p = 0.019). At T1, in treatment group, CK was lower than controls (p = 0.006), while RBC antioxidant capacity increased at rest (p = 0.037) and after exercise (p = 0.006) compared to T0. The dietary supplement showed efficacy in enhancing RBC antioxidant capacity, and it could be beneficial for horses engaged in intense aerobic exercise.
Copyright © 2025. Published by Elsevier Inc.
Publication Date: 2025-01-24 PubMed ID: 39864602DOI: 10.1016/j.jevs.2025.105364Google Scholar: Lookup
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Summary
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This research studied the impact of a dietary supplement, rich in natural antioxidants and omega-3 fatty acids, on the physical performance and blood antioxidant activity of Arabian endurance horses. It was concluded that the supplement was effective in boosting the antioxidant capacity of red blood cells, which may be beneficial for horses involved in intense aerobic sports.
Objective and Methodology of the Study
- The study aimed to examine the effect of a feed supplement composed of natural antioxidants and omega-3 fatty acids on the blood antioxidant function and athletic condition of endurance Arabian horses undergoing an exercise test.
- The study relied on a sample group of twelve Arabian endurance horses, which were randomly divided into a treatment group and a control group.
- At the starting point of the study (T0), several parameters such as blood lactate levels, antioxidant capacity of the whole blood and red blood cells (RBCs) were assessed. The horses were then put through an exercise test while their heart rates were monitored.
- Thirty minutes post the exercise, the blood lactate, antioxidant capacity, and serum creatine kinase (CK) levels were measured. The treatment group horses were then subjected to the dietary supplement for a period of 21 days, while the control group maintained their regular diet.
- The protocol was repeated again (T1) and various parameters were compared within and between the two groups through statistical methods like two-way ANOVA and post-hoc tests.
Key Findings of the Study
- The research provided considerable evidence of a significant interaction between time and group effects on factors like serum CK, RBC antioxidant capacity at rest, and post-exercise.
- At the end of the experiment (T1), the dietary supplement group showed lower CK levels in comparison to the control group.
- Furthermore, the treatment group experienced an increase in RBC antioxidant capacity both at rest and after exercise compared to the baseline (T0).
- The research concluded that the dietary supplement enriched with antioxidants and omega-3 fatty acids was productive in enhancing the antioxidant capacity of RBCs.
- Thus, it was suggested that this could be beneficial for horses engaged in intense aerobic exercise as it potentially bolsters their athletic performance by combating oxidative stress.
Cite This Article
APA
Stucchi L, Rossi R, Mainardi E, Ferrucci F.
(2025).
Antioxidant capacity and athletic condition of endurance horses undergoing nutraceutical supplementation.
J Equine Vet Sci, 105364.
https://doi.org/10.1016/j.jevs.2025.105364 Publication
Researcher Affiliations
- Department of Veterinary Medicine, University of Sassari, via Vienna 2, 07100 Sassari, Italy. Electronic address: lstucchi@uniss.it.
- Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
- Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
- Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
Conflict of Interest Statement
Declaration of competing interest None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper.
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