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Home / Equine Research Bank / Braz J Microbiol / Antiviral activity of a Bacillus sp. P34 peptide against pat...
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014; 45(3); 1089-1094; doi: 10.1590/s1517-83822014000300043

Antiviral activity of a Bacillus sp. P34 peptide against pathogenic viruses of domestic animals.

Abstract: P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV were incubated for 6 h at 37 °C the viral titer reduced from 10(4.5) TCID50 to 10(2.75) TCID50, showing a percent of inhibition of 98.6%. In conclusion, our results demonstrated that P34 inhibited EAV and FHV-1 replication in infected cell cultures and it showed virucidal activity against EAV. Since there is documented resistance to the current drugs used against herpesviruses and there is no treatment for equine viral arteritis, it is advisable to search for new antiviral compounds to overcome these infections.
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Publication Date: 2014-10-09 PubMed ID: 25477947PubMed Central: PMC4204951DOI: 10.1590/s1517-83822014000300043Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the in vitro antiviral properties of peptide P34, an antimicrobial compound produced by a Bacillus strain isolated from a fish in the Brazilian Amazon. The study explores the effectiveness of P34 against a range of common animal viruses, finding notable success against the equine arteritis virus and feline herpesvirus type 1.

Introduction to the Peptide P34

  • The peptide P34, involved in the research, is an antimicrobial produce of the Bacillus species. The specific Bacillus strain producing P34 was found in the intestines of a fish within the Amazon basin in Brazil.
  • Previous studies suggested the peptide has antibacterial activity, thus, this research aimed to analyze its antiviral properties.

Aim and Methodology of The Study

  • The primary purpose of this study was to evaluate the effectiveness of P34 as an antiviral against several known pathogenic viruses affecting domestic animals. Virus types tested included canine adenovirus type 2, canine coronavirus, canine distemper virus, and more.
  • In vitro investigations were performed, meaning the experiments were conducted outside of a living organism, likely in a controlled, laboratory environment.

Significant Findings

  • The study found that peptide P34 showcased significant antiviral properties when applied to equine arteritis virus (EAV) and feline herpesvirus type 1 (FHV-1).
  • P34 managed to inhibit the replication of EAV by 99.9% and FHV-1 by 94.4%, representing a substantial reduction in the viruses’ ability to proliferate.
  • Additionally, a virucidal activity was noticed specifically against EAV, which means P34 is capable of deactivate or destroying this virus.

Impact and Future Implications

  • The results of this study highlight the potential of P34 as a source of new antiviral compounds, specifically against EAV and FHV-1.
  • This finding is crucial as there exist issues of resistance against the current drugs used to treat herpesviruses, coupled with the fact that there are no existent treatments for equine viral arteritis.
  • Therefore, continued exploration and development of P34 and likely compounds could pave the way for new and more effective treatments against such challenging infections.

Cite This Article

APA
Scopel e Silva D, de Castro CC, da Silva e Silva F, Sant'anna V, Vargas GD, de Lima M, Fischer G, Brandelli A, da Motta Ade S, Hübner Sde O. (2014). Antiviral activity of a Bacillus sp. P34 peptide against pathogenic viruses of domestic animals. Braz J Microbiol, 45(3), 1089-1094. https://doi.org/10.1590/s1517-83822014000300043

Publication

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
NlmUniqueID: 101095924
Country: Brazil
Language: English
Volume: 45
Issue: 3
Pages: 1089-1094

Researcher Affiliations

Scopel e Silva, Débora
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
de Castro, Clarissa Caetano
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
da Silva e Silva, Fábio
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Sant'anna, Voltaire
  • Departamento de Ciência e Tecnologia de Alimentos Universidade Estadual do Rio Grande do Sul EncantadoRS Brazil Departamento de Ciência e Tecnologia de Alimentos, Universidade Estadual do Rio Grande do Sul, Encantado, RS, Brazil.
Vargas, Gilberto D'Avila
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
de Lima, Marcelo
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Fischer, Geferson
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Brandelli, Adriano
  • Laboratório de Bioquímica e Microbiologia Aplicada Departamento de Ciência de Alimentos Instituto de Ciência e Tecnologia de Alimentos Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
da Motta, Amanda de Souza
  • Instituto de Ciências Básicas da Saúde Departamento de Microbiologia Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Hübner, Silvia de Oliveira
  • Laboratório de Virologia e Imunologia Animal Faculdade de Veterinária Universidade Federal de Pelotas PelotasRS Brazil Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

MeSH Terms

  • Animals
  • Animals, Domestic / virology
  • Antimicrobial Cationic Peptides / isolation & purification
  • Antimicrobial Cationic Peptides / pharmacology
  • Antiviral Agents / isolation & purification
  • Antiviral Agents / pharmacology
  • Bacillus / isolation & purification
  • Bacillus / metabolism
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / pharmacology
  • Brazil
  • Fishes / microbiology
  • Gastrointestinal Tract / microbiology
  • Microbial Viability / drug effects
  • Temperature
  • Time Factors
  • Viral Load
  • Virus Replication / drug effects
  • Viruses / drug effects

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