Antiviral effect of recombinant equine interferon-gamma on several equine viruses.
Abstract: Recombinant equine interferon-gamma (reIFN-gamma) was prepared using a baculovirus expression system and its antiviral activity was investigated using several equine viruses. The reIFN-gamma suppressed the replication of all equine viruses used in the present experiment in horse cell cultures, but did not affect the growth of host cells at concentrations of less than 1000 u/ml. A strong antiviral effect was observed, especially against RNA viruses. Equine picornavirus, equine rhinovirus and equine arteritis virus could not be propagated at all in 100 u/ml reIFN-gamma when 100 TCID(50) of infective viruses was inoculated to cultivated horse cells. DNA viruses, equine herpesvirus types 1, 2, 3 and 4 and equine adenovirus, were less sensitive to reIFN-gamma but their growth became less than 1/100 in the cells treated with 100 u/ml reIFN-gamma compared to untreated cells. The antiviral effects were decreased in the cells of heterologous species and more than 1000 u/ml reIFN-gamma was required to induce an antiviral effect.
Copyright 2009 Elsevier B.V. All rights reserved.
Publication Date: 2009-11-18 PubMed ID: 20004981DOI: 10.1016/j.vetimm.2009.11.006Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article details the effects of using recombinant equine interferon-gamma (reIFN-gamma), produced through a baculovirus expression system, to combat various equine viruses, demonstrating a strong suppressive effect on viral replication in horse cells.
About the Interferon
- The study emphasizes the use of a specialized protein, recombinant equine interferon-gamma (reIFN-gamma).
- This protein was created using a baculovirus expression system, a popular method for producing eukaryotic proteins in high quantities.
- reIFN-gamma belongs to the group of interferons, which are proteins naturally released by host cells in response to the presence of pathogens such as viruses, bacteria or parasites.
Interferon’s Antiviral Activity
- The antiviral activity of reIFN-gamma was tested on several equine (horse) viruses.
- The research found that reIFN-gamma managed to suppress the replication of all tested equine viruses in horse cell cultures.
- Significantly, it did not affect the growth of host cells at concentrations less than 1000 u/ml.
Specific Effects on Different Viruses
- The research showed that the effectiveness of reIFN-gamma varied depending on the type of virus at hand.
- A strong antiviral effect was observed, especially against RNA viruses. These include equine picornavirus, equine rhinovirus, and equine arteritis virus, all of which could not be propagated at all in the presence of 100 u/ml reIFN-gamma.
- DNA viruses, namely equine herpesvirus types 1, 2, 3 and 4, and equine adenovirus, were less sensitive to reIFN-gamma.
- However, even these DNA viruses experienced reduced growth, becoming less than 1/100 in the presence of 100 u/ml of reIFN-gamma.
Interferon’s Potency in Heterologous Species
- The potency of the reIFN-gamma was observed to decrease when applied to cells of heterologous species.
- In these cases, more than 1000 u/ml of reIFN-gamma was required to induce a noticeable antiviral effect.
Cite This Article
APA
Sentsui H, Wu D, Murakami K, Kondo T, Matsumura T.
(2009).
Antiviral effect of recombinant equine interferon-gamma on several equine viruses.
Vet Immunol Immunopathol, 135(1-2), 93-99.
https://doi.org/10.1016/j.vetimm.2009.11.006 Publication
Researcher Affiliations
- National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan. Electronic address: sentusi.hiroshi@nihon-u.ac.jp.
- National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan; National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 15000, China.
- National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
- Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Shimotsuke, Tochigi 329-0412, Japan.
MeSH Terms
- Adenoviridae / drug effects
- Alphavirus / drug effects
- Animals
- Antiviral Agents / adverse effects
- Antiviral Agents / pharmacology
- Aphthovirus / drug effects
- Cells, Cultured
- Dose-Response Relationship, Drug
- Equartevirus / drug effects
- Herpesvirus 1, Equid / drug effects
- Herpesvirus 3, Equid / drug effects
- Herpesvirus 4, Equid / drug effects
- Horse Diseases / drug therapy
- Horse Diseases / virology
- Horses / virology
- Interferon-gamma / adverse effects
- Interferon-gamma / pharmacology
- Picornaviridae / drug effects
- Recombinant Proteins
- Rhadinovirus / drug effects
- Virus Replication / drug effects
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