Application of the ASVCP guidelines for the establishment of haematologic and biochemical reference intervals in Icelandic horses in Austria.
Abstract: Despite the increasing popularity of Icelandic horses, published reference intervals (RIs) in this breed are rare. Due to their isolation and their small gene pool, alterations in some variables are likely and some possible breed-specific peculiarities have been described. The purpose of the present study was the establishment of comprehensive RIs in Icelandic horses according to recently published guidelines. In a prospective observational study, blood samples were collected from the jugular vein of 142 Icelandic horses into EDTA and serum tubes. Reference intervals were established for haematologic and biochemical analytes on the Advia 2120i™ and the Dimension ExL™ by established methods. RIs were defined as central 95 % intervals bounded by the 2.5th and 97.5th percentiles with their 90 % confidence intervals, calculated according to recently published ASVCP guidelines. An inhouse-developed quality control system using observed total allowable error was used for the surveillance of the internal quality control preceding the measurements. Results: The RIs were as follows: haematocrit: 0.29-0.39, RBC: 5.79-8.63 T/l, haemoglobin: 102.0-142.3 g/l, MCV: 42-51 fl, platelets: 146-263 G/l, WBC: 4.13-8.57 G/l, segs: 1.98-4.73 G/l, lymphocytes: 1.25-3.49 G/l, monocytes: 0.06-0.31 G/l, eosinophils: 0.04-0.50 G/l, glucose: 4.0-5.7 mmol/l, urea: 3.2-6.4 mmol/l, creatinine: 79.6-141.4 μmol/l, total protein: 54.4-72.9 g/l, albumin: 27.7-36.8 g/l, total bilirubin: 8.1-21.1 μmol/l, triglycerides: 0.03-0.44 mmol/l, cholesterol: 1.75-2.90 mmol/l, ALP: 1.35-3.55 μkat/l, AST: 4.52-8.80 μkat/l, GLDH: 0.0-0.18 μkat/l, GGT: 0.11-0.39 μkat/l, CK: 2.53-6.52 μkat/l, LDH: 3.32-7.95 μkat/l, iron: 16.4-39.9 μmol/l, calcium: 2.69-3.19 mmol/l, phosphate: 0.5-1.3 mmol/l, magnesium: 0.6-0.9 mmol/l, sodium: 134-141 mmol/l, potassium: 3.6-4.7 mmol/l, chloride: 100-105 mmol/l. Conclusions: Reference intervals of several haematologic and biochemical analytes differed from the transferred historical reference intervals applied to equine samples in the authors' laboratory. These might be of clinical importance in some analytes such as creatine kinase.
Publication Date: 2015-06-14 PubMed ID: 26070944PubMed Central: PMC4466868DOI: 10.1186/s13028-015-0120-4Google Scholar: Lookup
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Summary
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The research paper focuses on establishing comprehensive reference intervals (RIs) for various haematologic and biochemical analytes in Icelandic horses using newly published guidelines.
Objective of the Study
The research aims at establishing the haematologic and biochemical reference intervals specific to Icelandic horses. The need for this research was triggered by:
- The increasing popularity of Icelandic horses, and;
- The paucity of published reference intervals (RIs) for this breed, despite some previously documented breed-specific peculiarities.
Methodology of the Study
The study used a prospective observational methodology. Researchers:
- Collected blood samples from the jugular vein of 142 Icelandic horses into Ethylenediaminetetraacetic acid (EDTA) and serum tubes;
- Performed established tests on the Advia 2120i™ and the Dimension ExL™;
- Defined reference intervals as central 95% intervals bounded by the 2.5th and 97.5th percentiles along with their 90% confidence intervals;
- Followed the latest guidelines published by the American Society for Veterinary Clinical Pathology (ASVCP) for calculation;
- Monitored the quality of the tests using an in-house developed quality control system.
Results of the Study
The research resulted in defining reference intervals for various haematologic and biochemical analytes in Icelandic horses. These include:
- Haematologic analytes: haematocrit, red blood cells (RBC), haemoglobin, mean corpuscular volume (MCV), platelets, white blood cells (WBC), segmented neutrophils (segs), lymphocytes, monocytes, eosinophils;
- Biochemical analytes: glucose, urea, creatinine, total protein, albumin, total bilirubin, triglycerides, cholesterol, alkaline phosphatase (ALP), aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), gamma-glutamyltransferase (GGT), creatine kinase (CK), lactate dehydrogenase (LDH), iron, calcium, phosphate, magnesium, sodium, potassium, chloride.
Conclusion of the Study
The findings revealed that the reference intervals for certain haematologic and biochemical analytes were different from the traditional reference intervals used for equine samples in the authors’ laboratory. The difference might have clinical importance in some cases, such as with creatine kinase. These findings emphasize the need for breed-specific reference intervals in the medical evaluation of horses.
Cite This Article
APA
Leidinger EF, Leidinger J, Figl J, Rumpler B, Schwendenwein I.
(2015).
Application of the ASVCP guidelines for the establishment of haematologic and biochemical reference intervals in Icelandic horses in Austria.
Acta Vet Scand, 57(1), 30.
https://doi.org/10.1186/s13028-015-0120-4 Publication
Researcher Affiliations
- In Vitro Veterinary Laboratories, Rennweg 95, 1030, Vienna, Austria. ernst.leidinger@invitro.at.
- In Vitro Veterinary Laboratories, Rennweg 95, 1030, Vienna, Austria. judith.leidinger@invitro.at.
- In Vitro Veterinary Laboratories, Rennweg 95, 1030, Vienna, Austria. juliafigl@gmx.net.
- Tierärztliche Praxis, Heiligenbrunnerg. 19, 7221, Marz, Austria. office@tierarzt-marz.at.
- Clinical Pathology Platform, University of Veterinary Medicine, Veterinärplatz 1, 1210, Vienna, Austria. ilse.schwendenwein@vetmeduni.ac.at.
MeSH Terms
- Animals
- Austria
- Blood Chemical Analysis / standards
- Blood Chemical Analysis / veterinary
- Female
- Guidelines as Topic
- Hematologic Tests / standards
- Hematologic Tests / veterinary
- Horses
- Male
- Prospective Studies
- Reference Values
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