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Journal of equine veterinary science2026; 106051; doi: 10.1016/j.jevs.2026.106051

Aqua training promotes changes of skeletal muscle mass in show jumping horses.

Abstract: The correct prescription of aquatic treadmill training for equine athletes depends on understanding the muscular changes generated by working in the water. Objective: The aim of this study was to test the hypothesis that the inclusion of a water treadmill in a routine training protocol would alter muscle mass of show jumping horses. Methods: Six fit-to-compete horses were trained for 10 weeks with a water treadmill (twice a week, 20 minutes, water level at the carpus), in addition to regular physical exercise. Body composition, infrared thermography (IRT) of propulsive muscles (m. brachiocephalic, m. semitendinosus and m. semimembranosus) and field jumping tests for aspartate aminotransferase (AST) and creatine kinase (CK) analysis and kinematic parameters were assessed before and after the protocol. Data was analyzed using paired t-test (p<0.05). Results: Body weight altered (550.5 ± 14.5 vs. 587.0 ± 41.9 kg), related to a fat-free mass gain (485.1 ± 19.1 vs. 520.1 ± 42.2 kg). Fat mass remained unchanged (65.4 ± 11.4 vs. 66.9 ± 10.3 kg), as no significant changes were observed for rump fat thickness (0.69 ± 0.45 vs. 0.59 ± 0.40 cm). IRT temperature of the selected muscles and AST and CK levels increased after training. Improvements in strike power (2.53 ± 0.38 vs. 3.18 ± 0.38 G), take-off angle (20.0 ± 5.0° vs. 24.5 ± 5.4°) and the aerial phase duration (665.3 ± 179.2 vs. 765.3 ± 41.1 ms) for the vertical jumps and in take-off angle (21.7 ± 6.4° vs. 26.1 ± 5.8°) for oxer jumps. Conclusions: These results suggest that the inclusion of the water treadmill promoted muscular recruitment and gain in muscle mass, contributing to a greater strength in the core muscles, to elevate the forehand, and in the propulsive hind limb muscles, to increase strike power.
Publication Date: 2026-06-06 PubMed ID: 42252004DOI: 10.1016/j.jevs.2026.106051Google Scholar: Lookup
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Summary

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Overview

Introduction and Objective

  • The correct use of aquatic treadmill training for competitive horses requires understanding how water-based exercise affects muscle development.
  • The study aimed to test whether incorporating water treadmill training into a horse’s regular routine changes muscle mass and improves muscular function relevant to show jumping.

Methods

  • Subjects: Six show jumping horses in competition-ready fitness condition.
  • Training Intervention: Each horse underwent 10 weeks of training, including 20-minute sessions twice weekly on an aquatic treadmill with water level at the carpus (horse’s wrist area).
  • Regular Exercise: Horses continued their usual physical training alongside the aquatic treadmill work.
  • Assessments conducted before and after the training period included:
    • Body composition measurements focusing on body weight, fat-free mass, and fat mass.
    • Infrared thermography (IRT) to measure surface temperature of key propulsive muscles: brachiocephalic, semitendinosus, semimembranosus.
    • Blood enzyme tests (AST and CK) to monitor muscle activity and recovery after field jumping tests.
    • Kinematic parameters of jumping such as strike power, take-off angle, and aerial phase duration for vertical and oxer jumps.
  • Statistical analysis: Paired t-tests were used to evaluate changes before and after the intervention with significance level p<0.05.

Results

  • Body composition:
    • Body weight increased significantly from approximately 550.5 kg to 587.0 kg.
    • Fat-free mass (muscle and lean tissue) increased significantly from about 485.1 kg to 520.1 kg.
    • Fat mass stayed stable around 65-67 kg, with no significant change in rump fat thickness, indicating muscle gain rather than fat gain.
  • Muscle activity and biochemistry:
    • Infrared thermography showed increased temperature in the brachiocephalic, semitendinosus, and semimembranosus muscles after training, suggesting greater muscle recruitment or blood flow.
    • Both aspartate aminotransferase (AST) and creatine kinase (CK) levels rose post-training, which are markers linked to muscle metabolism and response to exercise.
  • Jumping performance:
    • Vertical jumps showed improvements in strike power (force of impact with ground), from 2.53 G to 3.18 G.
    • Take-off angle increased significantly for vertical jumps (20° to 24.5°) and oxer jumps (21.7° to 26.1°), indicating better jump technique or strength.
    • Duration of the aerial phase (time in the air) in vertical jumps lengthened from 665.3 ms to 765.3 ms, reflecting enhanced jumping ability.

Conclusions

  • Adding water treadmill sessions to regular training promotes muscular recruitment in key propulsive muscles of show jumping horses.
  • The significant gain in muscle mass is primarily in fat-free tissue, implying improved muscle development rather than fat accumulation.
  • Enhanced muscle strength contributes to better take-off power, jump angles, and aerial phase duration, all indicators of improved jumping performance.
  • The training likely improves core strength to elevate the forehand (front of the horse) and strengthens hind limb muscles responsible for propulsion.
  • Overall, aquatic treadmill training appears to be an effective supplement to traditional conditioning for competitive show jumping horses.

Cite This Article

APA
Nascimento C, Santos C, Walton M, Gonçalves T, Freire G, Nicolau M, Silvestre F, Simões J, Borges J, Pires N, Filho HM, Clayton H, Coelho C. (2026). Aqua training promotes changes of skeletal muscle mass in show jumping horses. J Equine Vet Sci, 106051. https://doi.org/10.1016/j.jevs.2026.106051

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Pages: 106051
PII: S0737-0806(26)00286-8

Researcher Affiliations

Nascimento, C
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal; Hidrovet - Centro de Reabilitação Equina, Rua do Alecrim S/N, 2710-295 Janas, Portugal.
Santos, C
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal.
Walton, M
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal.
Gonçalves, T
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal.
Freire, G
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal; Hidrovet - Centro de Reabilitação Equina, Rua do Alecrim S/N, 2710-295 Janas, Portugal.
Nicolau, M
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal; Hidrovet - Centro de Reabilitação Equina, Rua do Alecrim S/N, 2710-295 Janas, Portugal.
Silvestre, F
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal; Hidrovet - Centro de Reabilitação Equina, Rua do Alecrim S/N, 2710-295 Janas, Portugal.
Simões, J
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal; Research in Veterinary Medicine (I-MVET), Faculty of Veterinary Medicine, Lusófona University, Lisbon University Centre, Campo Grande 376, 1749-024 Lisbon, Portugal.
Borges, J
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal.
Pires, N
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal.
Filho, H Manso
  • Núcleo de Pesquisa Equina, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manuel de Medeiros S/N, 51171-900 Recife, Pernambuco, Brazil.
Clayton, H
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, 48824 MI, United States.
Coelho, C
  • Faculty of Veterinary Medicine, Lusofona University, Campo Grande 376, 1749-024 Lisbon, Portugal; Research in Veterinary Medicine (I-MVET), Faculty of Veterinary Medicine, Lusófona University, Lisbon University Centre, Campo Grande 376, 1749-024 Lisbon, Portugal; MED - Mediterranean Institute for Agriculture, Environment and Development, Universidade de Évora, 7006-554 Évora, Portugal.. Electronic address: clarisse.coelho@ulusofona.pt.

Conflict of Interest Statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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