FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Biomedicines2023; 11(6); 1602; doi: 10.3390/biomedicines11061602

Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes.

Abstract: Cartilage injury defects in animals and humans result in the development of osteoarthritis and the progression of joint deterioration. Cell isolation from equine hyaline cartilage and evaluation of their ability to repair equine joint cartilage injuries establish a new experimental protocol for an alternative approach to osteochondral lesions treatment. Chondrocytes (CCs), isolated from the autologous cartilage of the trachea, grown in the laboratory, and subsequently arthroscopically implanted into the lesion site, were used to regenerate a chondral lesion of the carpal joint of a horse. Biopsies of the treated cartilage taken after 8 and 13 months of implantation for histological and immunohistochemical evaluation of the tissue demonstrate that the tissue was still immature 8 months after implantation, while at 13 months it was organized almost similarly to the original hyaline cartilage. Finally, a tissue perfectly comparable to native articular cartilage was detected 24 months after implantation. Histological investigations demonstrate the progressive maturation of the hyaline cartilage at the site of the lesion. The hyaline type of tracheal cartilage, used as a source of CCs, allows for the repair of joint cartilage injuries through the neosynthesis of hyaline cartilage that presents characteristics identical to the articular cartilage of the original tissue.
Get Full Text
Publication Date: 2023-05-31 PubMed ID: 37371697PubMed Central: PMC10296174DOI: 10.3390/biomedicines11061602Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores a revolutionary procedure used to treat equine cartilage injuries, using chondrocytes isolated from the horse’s hyaline cartilage. These chondrocyte cells were successfully cultivated and used to repair cartilage injuries in the joint of the horse, demonstrating a promising new treatment method for osteochondral lesions.

Objective of the Research

  • The research was conducted in response to the pervasive problem of cartilage injury defects in both animals and humans. These injuries often lead to the development of debilitating conditions like osteoarthritis and overall joint deterioration.
  • The aim was to create a new experimental protocol for treating osteochondral lesions in an innovative manner.

Methodology

  • Chondrocytes were isolated from the hyaline cartilage of the trachea in a horse, cultivated in a laboratory, and then arthroscopically implanted into the cartilage injury site (a lesion in the carpal joint). The researchers used these chondrocytes with the intention of regenerating the damaged cartilage.
  • The progress of the implanted chondrocytes was analyzed through biopsies, conducted after 8, 13, and 24 months, which were then examined through histological and immunohistochemical evaluations.

Findings of the Study

  • The tissue from the treated cartilage was found to be immature 8 months after chondrocyte implantation, but was largely akin to the original hyaline cartilage at the 13-month mark.
  • Notably, 24 months after the chondrocyte implantation, the regenerated tissue was practically identical to the horse’s original articular cartilage.
  • The study’s histological investigations highlighted the progressive maturity of the implanted hyaline cartilage at the site of the lesion over time.

Significance of the Research

  • This research paves the way for new treatment protocol for cartilage injuries, substituting the use of autologous cartilage with laboratory-grown chondrocytes.
  • The successful outcomes suggest the potential of this method, not only in equine models, but also in human application. If the results can be replicated, it would represent a significant breakthrough in osteochondral lesion treatment.

Cite This Article

APA
(2023). Articular Cartilage Regeneration by Hyaline Chondrocytes: A Case Study in Equine Model and Outcomes. Biomedicines, 11(6), 1602. https://doi.org/10.3390/biomedicines11061602

Publication

Biomedicines
ISSN: 2227-9059
NlmUniqueID: 101691304
Country: Switzerland
Language: English
Volume: 11
Issue: 6
PII: 1602

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 27 references
  1. Liu Y, Shah KM, Luo J. Strategies for Articular Cartilage Repair and Regeneration.. Front Bioeng Biotechnol 2021;9:770655.
    doi: 10.3389/fbioe.2021.770655pmc: PMC8719005pubmed: 34976967google scholar: lookup
  2. McIlwraith CW, Frisbie DD, Kawcak CE. The horse as a model of naturally occurring osteoarthritis.. Bone Joint Res 2012 Nov;1(11):297-309.
    doi: 10.1302/2046-3758.111.2000132pmc: PMC3626203pubmed: 23610661google scholar: lookup
  3. Baccarin RYA, Seidel SRT, Michelacci YM, Tokawa PKA, Oliveira TM. Osteoarthritis: a common disease that should be avoided in the athletic horse's life.. Anim Front 2022 Jun;12(3):25-36.
    doi: 10.1093/af/vfac026pmc: PMC9197312pubmed: 35711506google scholar: lookup
  4. Mirza U, Shubeena S, Shah M.S, Zaffer B, Jan A, Anjum S. Microfracture: A technique for repair of chondral defects.. J. Entomol. Zool. Stud. 2018;6:1092–1097.
  5. McIlwraith CW, Fortier LA, Frisbie DD, Nixon AJ. Equine Models of Articular Cartilage Repair.. Cartilage 2011 Oct;2(4):317-26.
    doi: 10.1177/1947603511406531pmc: PMC4297134pubmed: 26069590google scholar: lookup
  6. Van Hecke L, Magri C, Duchateau L, Beerts C, Geburek F, Suls M, Da Dalt L, Patruno M, Saunders J, Broeckx SY, Depuydt E, Spaas JH. Repeated intra-articular administration of equine allogeneic peripheral blood-derived mesenchymal stem cells does not induce a cellular and humoral immune response in horses.. Vet Immunol Immunopathol 2021 Sep;239:110306.
    doi: 10.1016/j.vetimm.2021.110306pubmed: 34365135google scholar: lookup
  7. Cokelaere S, Malda J, van Weeren R. Cartilage defect repair in horses: Current strategies and recent developments in regenerative medicine of the equine joint with emphasis on the surgical approach.. Vet J 2016 Aug;214:61-71.
    doi: 10.1016/j.tvjl.2016.02.005pubmed: 27387728google scholar: lookup
  8. Hunziker EB, Lippuner K, Keel MJ, Shintani N. An educational review of cartilage repair: precepts & practice--myths & misconceptions--progress & prospects.. Osteoarthritis Cartilage 2015 Mar;23(3):334-50.
    doi: 10.1016/j.joca.2014.12.011pubmed: 25534362google scholar: lookup
  9. Fugazzola MC, van Weeren PR. Surgical osteochondral defect repair in the horse-a matter of form or function?. Equine Vet J 2020 Jul;52(4):489-499.
    doi: 10.1111/evj.13231pmc: PMC7317185pubmed: 31958175google scholar: lookup
  10. Bourebaba L, Röcken M, Marycz K. Osteochondritis dissecans (OCD) in Horses - Molecular Background of its Pathogenesis and Perspectives for Progenitor Stem Cell Therapy.. Stem Cell Rev Rep 2019 Jun;15(3):374-390.
    doi: 10.1007/s12015-019-09875-6pmc: PMC6534522pubmed: 30796679google scholar: lookup
  11. McIlwraith C.W., Nixon A.J., Wright I.M., Boening K.J.. Diagnostic and Surgical Arthroscopy in the Horse.. 3rd ed. Elsevier; Amsterdam, The Netherlands: 2005. Arthroscopic methods 1009 for cartilage repair; pp. 455–742.
  12. Apostolakos JM, Lazaro L, Williams RJ. The Use of Bone Marrow Concentrate in the Treatment of Full-Thickness Chondral Defects.. HSS J 2019 Feb;15(1):96-99.
    doi: 10.1007/s11420-018-9647-zpmc: PMC6384207pubmed: 30863240google scholar: lookup
  13. Davies RL, Kuiper NJ. Regenerative Medicine: A Review of the Evolution of Autologous Chondrocyte Implantation (ACI) Therapy.. Bioengineering (Basel) 2019 Mar 13;6(1).
    doi: 10.3390/bioengineering6010022pmc: PMC6466051pubmed: 30871236google scholar: lookup
  14. McIlwraith CW, Frisbie DD. Microfracture: Basic Science Studies in the Horse.. Cartilage 2010 Apr;1(2):87-95.
    doi: 10.1177/1947603510367427pmc: PMC4297049pubmed: 26069539google scholar: lookup
  15. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
    doi: 10.3390/ani11010234pmc: PMC7832834pubmed: 33477808google scholar: lookup
  16. Lo Monaco M, Merckx G, Ratajczak J, Gervois P, Hilkens P, Clegg P, Bronckaers A, Vandeweerd JM, Lambrichts I. Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures.. Stem Cells Int 2018;2018:9079538.
    doi: 10.1155/2018/9079538pmc: PMC5832141pubmed: 29535784google scholar: lookup
  17. Urlić I, Ivković A. Cell Sources for Cartilage Repair-Biological and Clinical Perspective.. Cells 2021 Sep 21;10(9).
    doi: 10.3390/cells10092496pmc: PMC8468484pubmed: 34572145google scholar: lookup
  18. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know.. Front Vet Sci 2018;5:70.
    doi: 10.3389/fvets.2018.00070pmc: PMC5911772pubmed: 29713634google scholar: lookup
  19. Zha K, Li X, Yang Z, Tian G, Sun Z, Sui X, Dai Y, Liu S, Guo Q. Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application.. NPJ Regen Med 2021 Mar 19;6(1):14.
    doi: 10.1038/s41536-021-00122-6pmc: PMC7979687pubmed: 33741999google scholar: lookup
  20. Daou F, Cochis A, Leigheb M, Rimondini L. Current Advances in the Regeneration of Degenerated Articular Cartilage: A Literature Review on Tissue Engineering and Its Recent Clinical Translation.. Materials (Basel) 2021 Dec 21;15(1).
    doi: 10.3390/ma15010031pmc: PMC8745794pubmed: 35009175google scholar: lookup
  21. Shaz B.H., Hilley C.D., Gil M.R.. Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects.. 3rd ed. Elsevier; Amsterdam, The Netherlands: 2019. pp. 219–223.
  22. Watkins A, Fasanello D, Stefanovski D, Schurer S, Caracappa K, D'Agostino A, Costello E, Freer H, Rollins A, Read C, Su J, Colville M, Paszek M, Wagner B, Reesink H. Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models.. BMC Vet Res 2021 May 12;17(1):189.
    doi: 10.1186/s12917-021-02873-2pmc: PMC8117281pubmed: 33980227google scholar: lookup
  23. Dozin B, Malpeli M, Cancedda R, Bruzzi P, Calcagno S, Molfetta L, Priano F, Kon E, Marcacci M. Comparative evaluation of autologous chondrocyte implantation and mosaicplasty: a multicentered randomized clinical trial.. Clin J Sport Med 2005 Jul;15(4):220-6.
    doi: 10.1097/01.jsm.0000171882.66432.80pubmed: 16003035google scholar: lookup
  24. Armiento AR, Alini M, Stoddart MJ. Articular fibrocartilage - Why does hyaline cartilage fail to repair?. Adv Drug Deliv Rev 2019 Jun;146:289-305.
    doi: 10.1016/j.addr.2018.12.015pubmed: 30605736google scholar: lookup
  25. Żylińska B, Silmanowicz P, Sobczyńska-Rak A, Jarosz Ł, Szponder T. Treatment of Articular Cartilage Defects: Focus on Tissue Engineering.. In Vivo 2018 Nov-Dec;32(6):1289-1300.
    doi: 10.21873/invivo.11379pmc: PMC6365730pubmed: 30348681google scholar: lookup
  26. Jammes M, Contentin R, Cassé F, Galéra P. Equine osteoarthritis: Strategies to enhance mesenchymal stromal cell-based acellular therapies.. Front Vet Sci 2023;10:1115774.
    doi: 10.3389/fvets.2023.1115774pmc: PMC9950114pubmed: 36846261google scholar: lookup
  27. Mustonen AM, Lehmonen N, Oikari S, Capra J, Raekallio M, Mykkänen A, Paakkonen T, Rilla K, Niemelä T, Nieminen P. Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.. Sci Rep 2022 Oct 20;12(1):17550.
    doi: 10.1038/s41598-022-21398-8pmc: PMC9585069pubmed: 36266410google scholar: lookup

Citations

This article has been cited 3 times.
  1. Barbaro K, Marconi G, Innocenzi E, Altigeri A, Zepparoni A, Monteleone V, Alimonti C, Marcoccia D, Ghisellini P, Rando C, Ottoboni S, Rau JV, Eggenhöffner R, Scicluna MT. Regenerative treatment of canine osteogenic lesions with Platelet-Rich Plasma and hydroxyapatite: a case report. Front Vet Sci 2024;11:1459714.
    doi: 10.3389/fvets.2024.1459714pubmed: 39376921google scholar: lookup
  2. Reis IL, Lopes B, Sousa P, Sousa AC, Rêma A, Caseiro AR, Briote I, Rocha AM, Pereira JP, Mendonça CM, Santos JM, Lamas L, Atayde LM, Alvites RD, Maurício AC. Case report: Equine metacarpophalangeal joint partial and full thickness defects treated with allogenic equine synovial membrane mesenchymal stem/stromal cell combined with umbilical cord mesenchymal stem/stromal cell conditioned medium. Front Vet Sci 2024;11:1403174.
    doi: 10.3389/fvets.2024.1403174pubmed: 38840629google scholar: lookup
  3. Canonici F, Marcoccia D, Bonini P, Monteleone V, Innocenzi E, Zepparoni A, Altigeri A, Caciolo D, Tofani S, Ghisellini P, Rando C, Pechkova E, Rau JV, Eggenhöffner R, Scicluna MT, Barbaro K. Arthroscopic Treatment of a Subchondral Bone Cyst via Stem Cells Application: A Case Study in Equine Model and Outcomes. Biomedicines 2023 Dec 14;11(12).
    doi: 10.3390/biomedicines11123307pubmed: 38137527google scholar: lookup
Subscribe to our newsletter
Join 99,780 horse owners like you who receive our email updates on horse health and nutrition.