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Home / Equine Research Bank / Reprod Domest Anim / Assessing the fertility of two mares cloned from the same fo...
Reproduction in domestic animals = Zuchthygiene2019; 55(1); 44-53; doi: 10.1111/rda.13584

Assessing the fertility of two mares cloned from the same founder animal.

Abstract: Two cloned mares, produced from the same sample of skin fibroblasts, were bred during four breeding seasons from their second year of age, as embryo donors, in exactly the same conditions, using the same stallions for both cloned mares. The aim of this study was to test the embryo donor potential of cloned mares and to compare the results obtained from two cloned mares of the same mare with other embryo donor mares (n = 31-39 per breeding season) at the same stud. For both cloned mares, 19 embryos were recovered by 43 collection attempts (44%) (7/22 for one; 12/21 for the other), 16 (84%) pregnancies (5/7 for one, 11/12 for the other) were obtained at day 14 post-ovulation (D14 ), and 12 (3/7 for one; 9/12 for the other) foals were born. One cloned mare was a less efficient donor mare than the other (p < .05), In control donor mares, 623 embryo collections were performed, with a recovery rate (80%-496/623) significantly higher than for cloned mares. The recovery rate in the subpopulation of 2-5-year-old control donor mares (same age of cloned mares) (89%-127/143) and The recovery rate in the subpopulation of 12 control mares bred with the seven same stallions as clones (55%-17/31), were both higher than for cloned mare (p < .05). The success rate of transfer was not different between embryos produced by cloned mares (84%-16/19) and those produced by control donor mares (79%-392/496). However, the foaling rate per embryo collection was significantly lower for cloned mares (28%-12/43) than for control donor mares (52% - 325/623) (p < .05).
© 2019 Blackwell Verlag GmbH.
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Publication Date: 2019-12-07 PubMed ID: 31688983DOI: 10.1111/rda.13584Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research observed the fertility and embryo donation abilities of two cloned mares, which were bred over four seasons and compared to a control group of uncloned mares. The study found significant differences in fertility rates and embryo survival between the cloned mares and the control group.

Methodology

  • The research involved two cloned mares that were bred over four seasons, starting from their second year. These mares were cloned from the same skin fibroblast samples and bred in identical conditions, using the same stallions.
  • The team performed 43 collection attempts on both cloned mares, yielding a total of 19 embryos.
  • The cloned mares’ fertility performance was compared to a control group of uncloned, embryo donor mares (between 31 to 39 per breeding season) at the same stud.

Results

  • Of the 19 embryos collected from the cloned mares, 16 or 84% led to pregnancies detected 14 days post-ovulation. From these pregnancies, 12 resulted in live foals.
  • One of the cloned mares was significantly less efficient as a donor than the other, demonstrating an inconsistency in fertility performance among cloned mares.
  • In the control group, there were 623 embryo collection attempts, with a recovery rate of 80% – significantly higher than that of the cloned mares.
  • The embryo recovery rate was even higher among certain subgroups of the control, specifically, in the 2-5-year-old mares (89%) and the 12 mares bred with the same seven stallions as the cloned mares (55%). Both rates were found to be higher than that of the cloned mares.

Conclusion

  • No significant difference was observed in the success rate of embryo transfers between the cloned mares and the control group. However, the foaling rate per embryo collection was significantly lower in the cloned mares compared to the uncloned mares.
  • The research hence suggests that cloned mares may not be as efficient as their natural counterparts in terms of fertility and embryo recovery rates.

Cite This Article

APA
Dordas-Perpinyà M, Pintart C, Terris H, Normandin L, Bruyas JF. (2019). Assessing the fertility of two mares cloned from the same founder animal. Reprod Domest Anim, 55(1), 44-53. https://doi.org/10.1111/rda.13584

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 1
Pages: 44-53

Researcher Affiliations

Dordas-Perpinyà, Marta
  • Theriogenology Unit, Nantes Veterinary College, Nantes cedex 03, France.
Pintart, Cécile
  • Theriogenology Unit, Nantes Veterinary College, Nantes cedex 03, France.
Terris, Hubert
  • Haras de Hus, Petit Mars, France.
Normandin, Laure
  • Haras de Hus, Petit Mars, France.
Bruyas, Jean-François
  • Theriogenology Unit, Nantes Veterinary College, Nantes cedex 03, France.

MeSH Terms

  • Animals
  • Clone Cells / physiology
  • Embryo Transfer / veterinary
  • Embryo, Mammalian
  • Embryonic Development
  • Female
  • Fertility / genetics
  • Fertility / physiology
  • Horses / embryology
  • Horses / genetics
  • Horses / physiology
  • Male
  • Pregnancy

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