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Archives of virology2010; 156(2); 275-283; doi: 10.1007/s00705-010-0857-z

Assessing the variability of Brazilian Vaccinia virus isolates from a horse exanthematic lesion: coinfection with distinct viruses.

Abstract: During the last bovine vaccinia (BV) outbreaks, several Vaccinia virus (VACV) strains were isolated and characterised, revealing significant polymorphisms between strains, even within conserved genes. Although the epidemiology of VACV has been studied in BV outbreaks, there is little data about the circulation of the Brazilian VACV isolates. This study describes the genetic and biological characterisation of two VACV isolates, Pelotas 1 virus (P1V) and Pelotas 2 virus (P2V), which were obtained concomitantly from a horse affected by severe cutaneous disease. Despite being isolated from the same exanthematic clinical sample, P1V and P2V showed differences in their plaque phenotype and in one-step growth curves. Moreover, P1V and P2V presented distinct virulence profiles in a BALB/c mouse model, as observed with other Brazilian VACV isolates. Sequencing and phylogenetic analysis of four different genes demonstrated that the isolates are segregated in different VACV clusters. Our results raise interesting questions about the diversity of VACV isolates in Brazil.
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Publication Date: 2010-11-16 PubMed ID: 21080203DOI: 10.1007/s00705-010-0857-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores the variability and characteristics of two Vaccinia virus strains, Pelotas 1 virus and Pelotas 2 virus, extracted from a horse affected by severe skin disease. The study finds noticeable differences between the two strains’ growth patterns, virulence profiles, and gene sequencing, suggesting a greater diversity among Vaccinia virus strains in Brazil than previously known.

Exploring the Variability of Vaccinia Virus Strains

  • The paper notes that researchers have isolated and examined numerous Vaccinia virus (VACV) strains during recent bovine vaccinia outbreaks. These studies revealed substantial polymorphisms—or genetic variations—between the strains, even concerning genes typically regarded as conserved or unchanging.
  • However, the study points to a lack of data about the circulation of Brazilian VACV isolates, which this research aims to address by studying two VACV strains. These strains, dubbed Pelotas 1 virus (P1V) and Pelotas 2 virus (P2V), were isolated concurrently from a horse suffering from a severe cutaneous disease.

Differences between P1V and P2V Strains

  • Despite both strains being isolated from the same clinical sample, P1V and P2V exhibited differences in their plaque phenotype, which is a term used to describe the visible effects of a virus on infected cells observed in laboratory experiments.
  • The researchers also observed differences in one-step growth curves, suggesting the two virus strains multiply at different rates.
  • The virulence profiles—or the extent of the disease-causing capability—of P1V and P2V were distinct, mirroring the diversity found in other Brazilian VACV isolates.

Genetic Divergences

  • Sequencing and subsequent phylogenetic analysis of four different genes demonstrated that these isolates fall into different VACV clusters. This segregation suggests a more extensive genetic divergence between the strains.

Significance of the Study

  • This research reveals that there is a greater diversity among VACV isolates in Brazil than previously understood, posing interesting questions that need further investigation. It could potentially lead to more effective outbreak control strategies and nuanced vaccine development, given the considerable genetic variation and differing virulence found even among viruses isolated from the same sample.

Cite This Article

APA
Campos RK, Brum MC, Nogueira CE, Drumond BP, Alves PA, Siqueira-Lima L, Assis FL, Trindade GS, Bonjardim CA, Ferreira PC, Weiblen R, Flores EF, Kroon EG, Abrahão JS. (2010). Assessing the variability of Brazilian Vaccinia virus isolates from a horse exanthematic lesion: coinfection with distinct viruses. Arch Virol, 156(2), 275-283. https://doi.org/10.1007/s00705-010-0857-z

Publication

Archives of virology
ISSN: 1432-8798
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 156
Issue: 2
Pages: 275-283

Researcher Affiliations

Campos, Rafael K
  • Departamento de Microbiologia, Instituto de Ciências Biológicas, Laboratório de Vírus, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, Minas Gerais, Brazil.
Brum, Mário C S
    Nogueira, Carlos E W
      Drumond, Betânia P
        Alves, Pedro A
          Siqueira-Lima, Larissa
            Assis, Felipe L
              Trindade, Giliane S
                Bonjardim, Cláudio A
                  Ferreira, Paulo C
                    Weiblen, Rudi
                      Flores, Eduardo F
                        Kroon, Erna G
                          Abrahão, Jônatas S

                            MeSH Terms

                            • Amino Acid Sequence
                            • Animals
                            • Base Sequence
                            • Brazil
                            • Cattle
                            • DNA, Viral / genetics
                            • Exanthema / veterinary
                            • Exanthema / virology
                            • Genes, Viral
                            • Hemagglutinins, Viral / genetics
                            • Horse Diseases / virology
                            • Horses
                            • Male
                            • Mice
                            • Mice, Inbred BALB C
                            • Molecular Sequence Data
                            • Phylogeny
                            • Sequence Homology, Amino Acid
                            • Sequence Homology, Nucleic Acid
                            • Species Specificity
                            • Vaccinia / veterinary
                            • Vaccinia / virology
                            • Vaccinia virus / classification
                            • Vaccinia virus / genetics
                            • Vaccinia virus / isolation & purification
                            • Vaccinia virus / pathogenicity
                            • Virulence / genetics

                            Citations

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