Assessment of colostral transfer and systemic availability of immunoglobulin G in new-born foals using a newly developed enzyme-linked immunosorbent assay (ELISA) system.
Abstract: To measure the immunoglobulin G (IgG) concentration in colostrum, milk and serum samples, a sandwich enzyme-linked immunosorbent assay (ELISA) detection system was developed. The system provided high reproducibility and sensitivity for routine diagnostic purposes. The period of fluctuating serum concentrations of IgG was monitored in new-born foals and their mares for a period of 6 weeks postnatum and postpartum, respectively. All foals received colostrum from their mares. The mean IgG concentration in the precolostral mare serum was approximately 19.0 mg/ml and decreased significantly to 13.8 mg/ml within the first 24 h postpartum. The IgG value fell to a minimum of 11.2 mg/ml by day 21 and increased to 21.6 mg/ml by day 42 postpartum. Within the first 4 h postpartum, mean IgG concentrations of 54.5 mg/ml were measured in the colostrum. A significant decrease to 10.1 mg IgG/ml colostrum was then noted 9-12 h postpartum. The mean IgG concentrations in foal serum increased from 0.3 mg/ml (precolostral value) to 9.6 mg/ml within 5-8 h postnatum. After 13-16 h postnatum, the highest IgG value of 15.7 mg/ml was reached. Over time the mean IgG concentration decreased significantly to 7.9 mg/ml at day 35. At the end of the observation period (day 42 postnatum) the mean IgG concentration once again increased to 11.2 mg/ml serum. In addition, the possible influence of various parameters on IgG concentration were examined. No significant influences could be shown by the breed, mare age, number of pregnancies, days of gestation, month foaled, foal sex, or the different farms. Finally, the cumulative incidence of failure of passive transfer (FPT) defined as IgG levels < 4 mg/ml foal serum, and partial FPT (PFPT) at levels ranging from 4 to 8 mg/ml foal serum was determined. From a total of 70 foals, 10.0% showed FPT and 18.6% showed PFPT.
Publication Date: 2001-11-01 PubMed ID: 11686785DOI: 10.1046/j.1439-0396.2001.00313.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research investigated the concentration of immunoglobulin G (IgG) in the colostrum, milk, and serum samples of newborn foals and their mares using a newly developed enzyme-linked immunosorbent assay (ELISA) detection system. The study found no significant effects from breed, mare age, number of pregnancies, days of gestation, month foaled, foal sex, or the different farms on IgG concentration. Out of 70 foals, 10.0% demonstrated a failure of passive transfer (FPT), and 18.6% showed partial FPT.
Methodology
- The researchers developed a sandwich ELISA detection system to monitor IgG concentration in colostrum, milk, and serum samples.
- The system was noted for its high reproducibility and sensitivity, making it suitable for routine diagnostic purposes.
- IgG concentrations were monitored in newborn foals and their mares for six weeks after birth.
Findings
- The average IgG concentration in the precolostral mare serum was approximately 19.0 mg/ml, decreasing significantly to 13.8 mg/ml within the first 24 hours postpartum.
- The IgG value dropped to a minimum of 11.2 mg/ml by day 21 before rising again to 21.6 mg/ml by day 42 postpartum.
- Within the first four hours postpartum, mean IgG concentrations of 54.5 mg/ml were recorded in the colostrum. However, this concentration significantly decreased to 10.1 mg IgG/ml within 9-12 hours postpartum.
- Meanwhile, the average IgG concentrations in foal serum rose from 0.3 mg/ml (precolostral value) to 9.6 mg/ml within 5-8 hours postnatum. The highest level of 15.7 mg/ml was reached after 13-16 hours postnatum. Over time, the IgG concentration significantly decreased to 7.9 mg/ml at day 35 before increasing again to 11.2 mg/ml by day 42 postnatum.
- No significant influences on IgG concentration were found from the breed, mare age, number of pregnancies, days of gestation, month of foaling, the sex of the foal, or the difference between farms.
Failure of Passive Transfer (FPT)
- The study also identified the incidence of FPT, which is defined as IgG levels that are less than 4 mg/ml in foal serum. The incidence of partial FPT (PFPT), characterized by levels ranging from 4 to 8 mg/ml foal serum, was also examined.
- Out of a total of 70 foals, 10.0% presented with FPT, and 18.6% showed PFPT.
Cite This Article
APA
Erhard MH, Luft C, Remler HP, Stangassinger M.
(2001).
Assessment of colostral transfer and systemic availability of immunoglobulin G in new-born foals using a newly developed enzyme-linked immunosorbent assay (ELISA) system.
J Anim Physiol Anim Nutr (Berl), 85(5-6), 164-173.
https://doi.org/10.1046/j.1439-0396.2001.00313.x Publication
Researcher Affiliations
- Institute of Veterinary Physiology, Veterinary Faculty, University of Leipzig, Germany.
MeSH Terms
- Animals
- Animals, Newborn / blood
- Animals, Newborn / immunology
- Colostrum / immunology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Female
- Horses / blood
- Horses / immunology
- Immunity, Maternally-Acquired
- Immunoglobulin G / analysis
- Male
- Milk / immunology
- Reproducibility of Results
- Sensitivity and Specificity
- Time Factors
Citations
This article has been cited 5 times.- Rampacci E, Mazzola K, Beccati F, Passamonti F. Diagnostic characteristics of refractometry cut-off points for the estimation of immunoglobulin G concentration in mare colostrum.. Equine Vet J 2023 Jan;55(1):102-110.
- Turini L, Bonelli F, Nocera I, Battaglia F, Meucci V, Panzani D, Mele M, Sgorbini M. Evaluation of jennies' colostrum: IgG concentrations and absorption in the donkey foals. A preliminary study.. Heliyon 2020 Aug;6(8):e04598.
- Aoki T, Chiba A, Itoh M, Nambo Y, Yamagishi N, Shibano KI, Cheong SH. Colostral and foal serum immunoglobulin G levels and associations with perinatal abnormalities in heavy draft horses in Japan.. J Equine Sci 2020 Jun;31(2):29-34.
- Baptista VDS, Guttmann PM, Rusca AC, Moreira da Silva K, Macieira DB, de Alencar NX, Lessa DAB. Evaluation of acquired passive immunity in mule foals up to 60 days of age.. J Equine Sci 2020 Mar;31(1):1-4.
- Staubli D, Sager H, Haerdi C, Haessig M, Gottstein B. Precolostral serology in calves born from Neospora-seropositive mothers.. Parasitol Res 2006 Sep;99(4):398-404.
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