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Home / Equine Research Bank / BMC Vet Res / Assessment of Leishmania infantum infection in equine popula...
BMC veterinary research2019; 15(1); 381; doi: 10.1186/s12917-019-2108-1

Assessment of Leishmania infantum infection in equine populations in a canine visceral leishmaniosis transmission area.

Abstract: Leishmaniosis, zoonosis that produces significant public health impacts, is caused by Leishmania infantum. Canines are the main domestic reservoir and, besides humans, other species of mammals could be infected when living in endemic areas. In this study, we detected equine Leishmania infantum infections in a canine visceral leishmaniosis transmission area and evaluated the clinical, haematological, biochemical and oxidative stress disorders. This study was conducted in Uruguaiana, Rio Grande do Sul, south of Brazil. Peripheral blood samples were collected from 124 animals (98 horses and 26 dogs) of both genders and several breeds after they underwent general and dermatologic examinations. Results: Twenty five Leishmania infantum infected animals (20.16%), 14 horses and 11 dogs were detected by PCR (Polymerase Chain Reaction) amplification of kinetoplast DNA regions with 96% homology to Leishmania infantum (GenBank Accession No. L 19877.1). The clinical and haematological alterations of infected equines were skin lesions, nodules, lymphadenopathy, decreased levels in red blood cells and haematocrit (p < 0.05) and increase in urea serum concentration (p < 0.05), while CVL presented a decrease in red blood cells counts (p < 0.05), increase in lymphocytes (p < 0.05), and decrease in neutrophil-lymphocyte ratio (p  0.05) in both species. Conclusions: To our knowledge, this has been the first leishmaniosis equine survey performed in south of Brazil, caused by Leishmania infantum that were able to initially identify haematological and biochemical changes in the species, even in asymptomatic animals. We present evidence supporting those findings of haematological and biochemical changes could be related to infection. Surprisingly, the clinical manifestations of equine infection were similar to those found in canine visceral leishmaniosis. The equine population could be play an important role in the cycle of leishmaniosis in south Brazil and consequently indicates a great risk of public health. This evaluation of infected animals is important to establish the clinical and laboratory parameters involved in the disease progression.
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Publication Date: 2019-10-30 PubMed ID: 31666069PubMed Central: PMC6821015DOI: 10.1186/s12917-019-2108-1Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses a study conducted to assess the presence and effects of Leishmania infantum infections in horses, living in an area where canine visceral leishmaniosis is transmitted, in the southern region of Brazil. The study discovered changes in haematological and biochemical factors in the infected equines, similar to those found in dogs with the disease, even in asymptomatic animals.

Research Details

  • The study was conducted in Uruguaiana, Rio Grande do Sul, located in the southern part of Brazil.
  • A total of 124 animals, comprising 98 horses and 26 dogs of various breeds and both genders, were examined for Leishmania infantum infection.
  • These examinations included general check-ups and detailed dermatologic assessments.
  • For the purpose of the infection detection, peripheral blood samples were collected from all the animals and analysed using PCR (Polymerase Chain Reaction) method.

Findings

  • The researchers found that 25 out of 124 animals were infected with Leishmania infantum, this included 14 horses and 11 dogs.
  • The Leishmania infantum found had a 96% homology (similarity) to the reference collected in the GenBank with Accession Number L19877.1.
  • The equines displayed skin lesions, nodules, and lymphadenopathy, with lowered red blood cell levels and haematocrit, as well as increased serum concentration of urea.
  • The same symptoms were found in dogs with visceral leishmaniosis, including a decrease in red blood cells, increased lymphocytes, and a decreased neutrophil-lymphocyte ratio.
  • The research also performed tests for oxidative stress markers in the plasma protein carbonyl and plasma lipid peroxidation but found no significant changes.

Conclusions

  • This study is the first to survey equine leishmaniosis caused by Leishmania infantum in southern Brazil.
  • It found significant haematological and biochemical changes in infected horses, including in asymptomatic cases.
  • The clinical symptoms observed in the infected horses were similar to those found in dogs with visceral leishmaniosis.
  • The results suggest that the equine population could play a significant role in the cycle of leishmaniosis in southern Brazil, presenting a substantial public health risk.
  • Further assessments of infected animals are necessary to fully understand the clinical and laboratory parameters involved in the progression of the disease.

Cite This Article

APA
Escobar TA, Dowich G, Dos Santos TP, Zuravski L, Duarte CA, Lübeck I, Manfredini V. (2019). Assessment of Leishmania infantum infection in equine populations in a canine visceral leishmaniosis transmission area. BMC Vet Res, 15(1), 381. https://doi.org/10.1186/s12917-019-2108-1

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 381
PII: 381

Researcher Affiliations

Escobar, Taiane Acunha
  • Biochemistry Postgraduate Program, Federal University of Pampa, 118 BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil. taianeescobar@hotmail.com.
Dowich, Gabriela
  • Animal Science, Postgraduate, Federal University of Pampa, 118 BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil.
Dos Santos, Thália Pacheco
  • Pharmacy, Federal University of Pampa, 118 BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil.
Zuravski, Luísa
  • Biochemistry Postgraduate Program, Federal University of Pampa, 118 BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil.
Duarte, Claudia Acosta
  • Veterinary Medicine, Federal University of Pampa, 118, BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil.
Lübeck, Irina
  • Veterinary Medicine, Federal University of Pampa, 118, BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil.
Manfredini, Vanusa
  • Biochemistry Postgraduate Program, Federal University of Pampa, 118 BR 472, Uruguaiana, Rio Grande do Sul, Km 592, Brazil.

MeSH Terms

  • Animals
  • Brazil / epidemiology
  • DNA, Kinetoplast / genetics
  • Dog Diseases / blood
  • Dog Diseases / epidemiology
  • Dog Diseases / parasitology
  • Dogs
  • Horse Diseases / blood
  • Horse Diseases / epidemiology
  • Horse Diseases / parasitology
  • Horses
  • Leishmania infantum / genetics
  • Leishmania infantum / isolation & purification
  • Leishmaniasis, Visceral / epidemiology
  • Leishmaniasis, Visceral / parasitology
  • Leishmaniasis, Visceral / veterinary
  • Zoonoses

Grant Funding

  • Finance Code 001 / Coordenau00e7u00e3o de Aperfeiu00e7oamento de Pessoal de Nu00edvel Superior
  • Nu00ba 17/2551-001 405-7 by Research Program for SUS: Shared Health Management - PPSUS: DECIT/SCTIE/MS, CNPq, FAPERGS and SES-RS / Fundau00e7u00e3o de Amparo u00e0 Pesquisa do Estado do Rio Grande do Sul

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 38 references
  1. Deboni SC, Barbosa M, Ramos RR. Leishmaniose visceral no Rio Grande do Sul. Boletim Epidemiológico: Centro Estadual de Vigilância em Saúde 2011.
  2. Ready PD. Epidemiology of visceral leishmaniasis.. Clin Epidemiol 2014;6:147-54.
    doi: 10.2147/CLEP.S44267pmc: PMC4014360pubmed: 24833919google scholar: lookup
  3. CEVSRS - Centro Estadual de Vigilância em Saúde. Nota Técnica conjunta da Secretaria de Vigilância em Saúde do Ministério da Saúde e da Secretaria de Estado da Saúde do Rio Grande do Sul sobre a situação da Leishmaniose visceral na Fronteira oeste do Estado Rio Grande do Sul com a Argentina. Centro Estadual de Vigilância em Saúde 2010.
  4. SES. Secretaria de Saúde do Rio Grande do Sul. Nota Técnica conjunta n° 01/2014 - CEVS-IPB-LACEN- SES/RS: Leishmaniose visceral no Estado do Rio Grande do Sul. Secretaria de Saúde 2014.
  5. Roque AL, Jansen AM. Wild and synanthropic reservoirs of Leishmania species in the Americas.. Int J Parasitol Parasites Wildl 2014 Dec;3(3):251-62.
    doi: 10.1016/j.ijppaw.2014.08.004pmc: PMC4241529pubmed: 25426421google scholar: lookup
  6. Müller N, Hentrich B, Frey CF, Welle M. Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections.. Mol Cell Probes 2015 Dec;29(6):507-510.
    doi: 10.1016/j.mcp.2015.09.008pubmed: 26427730google scholar: lookup
  7. de Rezende MB, Herrera HM, Carvalho CME, Carvalho Anjos EA, Ramos CAN, de Araújo FR, Torres JM, de Oliveira CE. Detection of Leishmania spp. in Bats from an Area of Brazil Endemic for Visceral Leishmaniasis.. Transbound Emerg Dis 2017 Dec;64(6):e36-e42.
    doi: 10.1111/tbed.12597pubmed: 28233434google scholar: lookup
  8. Han S, Wu WP, Chen K, Osman I, Kiyim K, Zhao J, Hou YY, Wang Y, Wang LY, Zheng CJ. Epidemiological survey of sheep as potential hosts for Leishmania in China.. BMC Vet Res 2018 Dec 3;14(1):378.
    doi: 10.1186/s12917-018-1701-zpmc: PMC6276147pubmed: 30509251google scholar: lookup
  9. Kouam MK, Diakou A, Kanzoura V, Papadopoulos E, Gajadhar AA, Theodoropoulos G. A seroepidemiological study of exposure to Toxoplasma, Leishmania, Echinococcus and Trichinella in equids in Greece and analysis of risk factors.. Vet Parasitol 2010 May 28;170(1-2):170-5.
    doi: 10.1016/j.vetpar.2010.02.004pubmed: 20197215google scholar: lookup
  10. Rolão N, Martins MJ, João A, Campino L. Equine infection with Leishmania in Portugal.. Parasite 2005 Jun;12(2):183-6.
    doi: 10.1051/parasite/2005122183pubmed: 15991833google scholar: lookup
  11. Müller N, Welle M, Lobsiger L, Stoffel MH, Boghenbor KK, Hilbe M, Gottstein B, Frey CF, Geyer C, von Bomhard W. Occurrence of Leishmania sp. in cutaneous lesions of horses in Central Europe.. Vet Parasitol 2009 Dec 23;166(3-4):346-51.
    doi: 10.1016/j.vetpar.2009.09.001pubmed: 19800739google scholar: lookup
  12. Soares IR, Silva SO, Moreira FM, Prado LG, Fantini P, Maranhão Rde P, da Silva Filho JM, Melo MN, Palhares MS. First evidence of autochthonous cases of Leishmania (Leishmania) infantum in horse (Equus caballus) in the Americas and mixed infection of Leishmania infantum and Leishmania (Viannia) braziliensis.. Vet Parasitol 2013 Nov 8;197(3-4):665-9.
    doi: 10.1016/j.vetpar.2013.06.014pubmed: 23845306google scholar: lookup
  13. Truppel JH, Otomura F, Teodoro U, Massafera R, da Costa-Ribeiro MC, Catarino CM, Dalagrana L, Costa Ferreira ME, Thomaz-Soccol V. Can equids be a reservoir of Leishmania braziliensis in endemic areas?. PLoS One 2014;9(4):e93731.
    doi: 10.1371/journal.pone.0093731pmc: PMC3983081pubmed: 24721908google scholar: lookup
  14. Gama A, Elias J, Ribeiro AJ, Alegria N, Schallig HD, Silva F, Santarém N, Cardoso L, Cotovio M. Cutaneous leishmaniosis in a horse from northern Portugal.. Vet Parasitol 2014 Feb 24;200(1-2):189-92.
    doi: 10.1016/j.vetpar.2013.12.005pubmed: 24388338google scholar: lookup
  15. Koehler K, Stechele M, Hetzel U, Domingo M, Schönian G, Zahner H, Burkhardt E. Cutaneous leishmaniosis in a horse in southern Germany caused by Leishmania infantum.. Vet Parasitol 2002 Oct 16;109(1-2):9-17.
    doi: 10.1016/S0304-4017(02)00246-7pubmed: 12383621google scholar: lookup
  16. Feitosa FLF, Leal J, Mendes LCN, Peiro JR, Perri SHV, VMFd L. Estudo soroepidemiológico de leishmaniose em equinos na região de Araçatuba-SP, Brasil, área endêmica Para leishmaniose visceral. Braz J Vet Res Anim Sci 2012;49(6):500–502.
    doi: 10.11606/issn.1678-4456.v49i6p500-502google scholar: lookup
  17. Freitas JC, Nunes-Pinheiro DC, Lopes Neto BE, Santos GJ, Abreu CR, Braga RR, Campos Rde M, Oliveira LF. Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi.. Rev Soc Bras Med Trop 2012 Feb;45(1):24-9.
    doi: 10.1590/S0037-86822012000100006pubmed: 22370824google scholar: lookup
  18. Almeida AB, Sousa VR, Gasparetto ND, da Silva GF, Figueiredo FB, Dutra V, Nakazato L, Madeira MF. Canine visceral leishmaniasis: diagnostic approaches based on polymerase chain reaction employing different biological samples.. Diagn Microbiol Infect Dis 2013 Jul;76(3):321-4.
    doi: 10.1016/j.diagmicrobio.2013.03.017pubmed: 23619344google scholar: lookup
  19. Cerqueira EJ, Sherlock I, Gusmão A, Barbosa Júnior Ade A, Nakatani M. [Experimental infection of Equus asinus with Leishmania chagasi Cunha & Chagas, 1937].. Rev Soc Bras Med Trop 2003 Nov-Dec;36(6):695-701.
    doi: 10.1590/S0037-86822003000600009pubmed: 15049109google scholar: lookup
  20. Lopes AP, Sousa S, Dubey JP, Ribeiro AJ, Silvestre R, Cotovio M, Schallig HD, Cardoso L, Cordeiro-da-Silva A. Prevalence of antibodies to Leishmania infantum and Toxoplasma gondii in horses from the north of Portugal.. Parasit Vectors 2013 Jun 17;6:178.
    doi: 10.1186/1756-3305-6-178pmc: PMC3686701pubmed: 23773870google scholar: lookup
  21. Barroso PA, Nevot MC, Hoyos CL, Locatelli FM, Lauthier JJ, Ruybal P, Cardozo RM, Russo PD, Vassiliades CN, Mora MC, Estévez JO, Hashiguchi Y, Korenaga M, Basombrío MA, Marco JD. Genetic and clinical characterization of canine leishmaniasis caused by Leishmania (Leishmania) infantum in northeastern Argentina.. Acta Trop 2015 Oct;150:218-23.
    doi: 10.1016/j.actatropica.2015.08.007pubmed: 26277067google scholar: lookup
  22. Lima IS, Silva JS, Almeida VA, Junior FG, Souza PA, Larangeira DF, Moura-Neto JP, Fraga DB, de Freitas LA, dos-Santos WL. Severe clinical presentation of visceral leishmaniasis in naturally infected dogs with disruption of the splenic white pulp.. PLoS One 2014;9(2):e87742.
    doi: 10.1371/journal.pone.0087742pmc: PMC3911999pubmed: 24498367google scholar: lookup
  23. Fernández-Bellon H, Solano-Gallego L, Bardagí M, Alberola J, Ramis A, Ferrer L. Immune response to Leishmania infantum in healthy horses in Spain.. Vet Parasitol 2006 Jan 30;135(2):181-5.
    doi: 10.1016/j.vetpar.2005.09.007pubmed: 16213661google scholar: lookup
  24. Cardoso L, Lopes AP, Sherry K, Schallig H, Solano-Gallego L. Low seroprevalence of Leishmania infantum infection in cats from northern Portugal based on DAT and ELISA.. Vet Parasitol 2010 Nov 24;174(1-2):37-42.
    doi: 10.1016/j.vetpar.2010.08.022pubmed: 20851524google scholar: lookup
  25. Mahmoud MS, El-Ezz NT, Abdel-Shafy S, Nassar SA, El Namaky AH, Khalil WK, Knowles D, Kappmeyer L, Silva MG, Suarez CE. Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches.. Parasit Vectors 2016 May 4;9:260.
    pmc: PMC4857240pubmed: 27146413doi: 10.1186/s13071-016-1539-9google scholar: lookup
  26. Abbehusen MMC, Almeida VDA, Solcà MDS, Pereira LDS, Costa DJ, Gil-Santana L, Bozza PT, Fraga DBM, Veras PST, Dos-Santos WLC, Andrade BB, Brodskyn CI. Clinical and immunopathological findings during long term follow-up in Leishmania infantum experimentally infected dogs.. Sci Rep 2017 Nov 21;7(1):15914.
    pmc: PMC5698407pubmed: 29162847doi: 10.1038/s41598-017-15651-8google scholar: lookup
  27. Almeida BF, Narciso LG, Bosco AM, Pereira PP, Braga ET, Avanço SV, Marcondes M, Ciarlini PC. Neutrophil dysfunction varies with the stage of canine visceral leishmaniosis.. Vet Parasitol 2013 Sep 1;196(1-2):6-12.
    doi: 10.1016/j.vetpar.2013.02.016pubmed: 23498648google scholar: lookup
  28. Cohen G, Rudnicki M, Hörl WH. Uremic toxins modulate the spontaneous apoptotic cell death and essential functions of neutrophils.. Kidney Int Suppl 2001 Feb;78:S48-52.
    doi: 10.1046/j.1523-1755.2001.59780048.xpubmed: 11168982google scholar: lookup
  29. Almeida BF, Narciso LG, Melo LM, Preve PP, Bosco AM, Lima VM, Ciarlini PC. Leishmaniasis causes oxidative stress and alteration of oxidative metabolism and viability of neutrophils in dogs.. Vet J 2013 Dec;198(3):599-605.
    doi: 10.1016/j.tvjl.2013.08.024pubmed: 24080475google scholar: lookup
  30. Silva AC, de Almeida BF, Soeiro CS, Ferreira WL, de Lima VM, Ciarlini PC. Oxidative stress, superoxide production, and apoptosis of neutrophils in dogs with chronic kidney disease.. Can J Vet Res 2013 Apr;77(2):136-41.
    pmc: PMC3605930pubmed: 24082406
  31. Sarkari B, Naraki T, Ghatee MA, Abdolahi Khabisi S, Davami MH. Visceral Leishmaniasis in Southwestern Iran: A Retrospective Clinico-Hematological Analysis of 380 Consecutive Hospitalized Cases (1999-2014).. PLoS One 2016;11(3):e0150406.
    doi: 10.1371/journal.pone.0150406pmc: PMC4778872pubmed: 26942443google scholar: lookup
  32. Torrecilha RBP, Utsunomiya YT, Bosco AM, Almeida BF, Pereira PP, Narciso LG, Pereira DCM, Baptistiolli L, Calvo-Bado L, Courtenay O, Nunes CM, Ciarlini PC. Correlations between peripheral parasite load and common clinical and laboratory alterations in dogs with visceral leishmaniasis.. Prev Vet Med 2016 Sep 15;132:83-87.
    doi: 10.1016/j.prevetmed.2016.08.006pubmed: 27664450google scholar: lookup
  33. Reis AB, Teixeira-Carvalho A, Giunchetti RC, Guerra LL, Carvalho MG, Mayrink W, Genaro O, Corrêa-Oliveira R, Martins-Filho OA. Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.. Clin Exp Immunol 2006 Nov;146(2):303-11.
    doi: 10.1111/j.1365-2249.2006.03206.xpmc: PMC1942052pubmed: 17034583google scholar: lookup
  34. Sambrook J, Russel DW. Molecular cloning: a laboratory manual. 3. New York: Cold Spring Harbor; 2001.
  35. Reznick AZ, Packer L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay.. Methods Enzymol 1994;233:357-63.
    doi: 10.1016/S0076-6879(94)33041-7pubmed: 8015470google scholar: lookup
  36. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. Determination of carbonyl content in oxidatively modified proteins.. Methods Enzymol 1990;186:464-78.
    doi: 10.1016/0076-6879(90)86141-Hpubmed: 1978225google scholar: lookup
  37. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.. Anal Biochem 1979 Jun;95(2):351-8.
    doi: 10.1016/0003-2697(79)90738-3pubmed: 36810google scholar: lookup
  38. Brazil. Establish procedures for the scientific use for animals. Union Oficial Diary (Brazil Federal Constitucion) Law number 11.794/2008 s1.

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  1. Montaner-Angoiti E, Llobat L. Is leishmaniasis the new emerging zoonosis in the world?. Vet Res Commun 2023 Jul 12;.
    doi: 10.1007/s11259-023-10171-5pubmed: 37438495google scholar: lookup
  2. Mendes Junior AAV, Filgueira CPB, Miranda LFC, de Almeida AB, Cantanhêde LM, Fagundes A, Pereira SA, Menezes RC, Cupolillo E. First report of Leishmania (Mundinia) martiniquensis in South American territory and confirmation of Leishbunyavirus infecting this parasite in a mare.. Mem Inst Oswaldo Cruz 2023;118:e220220.
    doi: 10.1590/0074-02760220220pubmed: 37194810google scholar: lookup
  3. Ratzlaff FR, Osmari V, da Silva D, de Paula Vasconcellos JS, Pötter L, Fernandes FD, de Mello Filho JA, de Avila Botton S, Vogel FSF, Sangioni LA. Identification of infection by Leishmania spp. in wild and domestic animals in Brazil: a systematic review with meta-analysis (2001-2021).. Parasitol Res 2023 Jul;122(7):1605-1619.
    doi: 10.1007/s00436-023-07862-ypubmed: 37154922google scholar: lookup
  4. Álvarez L, Marín-García PJ, Llobat L. Genetic haplotypes associated with immune response to Leishmania infantum infection in dogs.. Vet Res Commun 2023 Sep;47(3):1675-1685.
    doi: 10.1007/s11259-023-10123-zpubmed: 37059873google scholar: lookup
  5. Álvarez L, Marín-García PJ, Rentero-Garrido P, Martinez-Jimenez CP, Llobat L. Interleukin 6 and interferon gamma haplotypes are related to cytokine serum levels in dogs in an endemic Leishmania infantum region.. Infect Dis Poverty 2023 Feb 10;12(1):9.
    doi: 10.1186/s40249-023-01058-3pubmed: 36759910google scholar: lookup
  6. Derghal M, Tebai A, Balti G, Souguir-Omrani H, Chemkhi J, Rhim A, Bouattour A, Guizani I, M'Ghirbi Y, Guerbouj S. High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.. Parasit Vectors 2022 Jan 8;15(1):12.
    doi: 10.1186/s13071-021-05138-xpubmed: 34996507google scholar: lookup
  7. Pradella GD, Duarte CA, Escobar TA, Zuravski L, Góss GC, Skupien JA, Lübeck I. Risk and protective factors of Leishmaniasis in the rural area of the western border region of Rio Grande do Sul, Brazil.. BMC Vet Res 2021 Oct 14;17(1):330.
    doi: 10.1186/s12917-021-03021-6pubmed: 34649576google scholar: lookup
  8. Ruggerone B, Scavone D, Troìa R, Giunti M, Dondi F, Paltrinieri S. Comparison of Protein Carbonyl (PCO), Paraoxonase-1 (PON1) and C-Reactive Protein (CRP) as Diagnostic and Prognostic Markers of Septic Inflammation in Dogs.. Vet Sci 2021 May 29;8(6).
    doi: 10.3390/vetsci8060093pubmed: 34072427google scholar: lookup
  9. González E, Molina R, Iriso A, Ruiz S, Aldea I, Tello A, Fernández D, Jiménez M. Opportunistic feeding behaviour and Leishmania infantum detection in Phlebotomus perniciosus females collected in the human leishmaniasis focus of Madrid, Spain (2012-2018).. PLoS Negl Trop Dis 2021 Mar;15(3):e0009240.
    doi: 10.1371/journal.pntd.0009240pubmed: 33720936google scholar: lookup
  10. Leonel JAF, Tannihão B, Arantes JA, Vioti G, Benassi JC, Brandi RA, Ferreira HL, Keid LB, Soares RM, Oliveira TMFS. Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR.. Rev Inst Med Trop Sao Paulo 2021;63:e12.
    doi: 10.1590/S1678-9946202163012pubmed: 33533815google scholar: lookup
  11. Morelli S, Colombo M, Dimzas D, Barlaam A, Traversa D, Di Cesare A, Russi I, Spoletini R, Paoletti B, Diakou A. Leishmania infantum Seroprevalence in Cats From Touristic Areas of Italy and Greece.. Front Vet Sci 2020;7:616566.
    doi: 10.3389/fvets.2020.616566pubmed: 33363245google scholar: lookup
  12. Gazzonis AL, Bertero F, Moretta I, Morganti G, Mortarino M, Villa L, Zanzani SA, Morandi B, Rinnovati R, Vitale F, Manfredi MT, Cardoso L, Veronesi F. Detecting antibodies to Leishmania infantum in horses from areas with different epizooticity levels of canine leishmaniosis and a retrospective revision of Italian data.. Parasit Vectors 2020 Oct 22;13(1):530.
    doi: 10.1186/s13071-020-04385-8pubmed: 33092640google scholar: lookup
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