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Veterinary medicine and science2022; 9(1); 158-166; doi: 10.1002/vms3.1051

Assessment of the microvascular perfusion using sidestream dark-field imaging in healthy newborn foals.

Abstract: Different methods to measure tissue perfusion are available in equine neonatology, but they are not representative of microvascular derangements. Objective: To evaluate the feasibility of the sidestream dark-field (SDF) capillaroscopy to visualize the capillary microvasculature in conscious newborn foals, the differences between two imaging sites and times of measurements, and the measurements' reproducibility. Methods: Seventeen healthy newborn foals were enrolled. Three sites at the upper and lower lip mucosa were assessed by SDF, using a hand-held capiscope, at 24 h and at 4-5 days after birth. Videos were assessed independently by two observers for quality and for semiquantitative calculation of microvascular parameters, including vascular density (VD), microvascular flow index (MFI), proportion of perfused vessels (PPV), and functional capillary density (FCD). Data were analyzed using unpaired and paired Student's T-tests to assess differences between sites and time-points. Bland-Altman plots and intraclass correlation coefficient (ICC) were used to assess measurement reproducibility. Results: Differences were found between the upper and the lower lip for VD at both 24 h and 4-5 days, and for FCD at 24 h, and between the two time-points for PPV at the lower lip. ICC for measurement reproducibility was good for all parameters (0.64-0.79) for the lower lip, and was good for VD and FCD (0,76-0,79) and fair to moderate for MFI and PPV (0.31-0.41) for the upper lip. Conclusions: Measurement of the capillary microvasculature is feasible in the conscious newborn foal. The lower lip has the best measurement reproducibility. Further investigations are warranted in cardiovascularly compromised cases, particularly in septic foals.
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
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Publication Date: 2022-12-16 PubMed ID: 36524796PubMed Central: PMC9857114DOI: 10.1002/vms3.1051Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the use of sidestream dark-field (SDF) capillaroscopy to measure microvascular perfusion in healthy newborn foals. It evaluates the reliability of the measurements and differences between two measurement sites and two time points.

Research Methods

  • The research included 17 healthy newborn foals.
  • The investigators utilized SDF capillaroscopy, a non-invasive imaging technique, which uses a handheld capiscope to visualize the capillary microvasculature in the upper and lower lip mucosa of the foals.
  • Readings were taken at 24 hours and 4-5 days after birth.
  • Microvascular parameters analyzed included vascular density (VD), microvascular flow index (MFI), proportion of perfused vessels (PPV), and functional capillary density (FCD).
  • The quality of the videos and the calculation of microvascular parameters were independently evaluated by two observers.
  • Student’s T-tests were used to evaluate differences between sites and time-points.
  • Measurement reproducibility was assessed using Bland-Altman plots and the intraclass correlation coefficient (ICC).

Research Findings

  • Results indicated differences between the upper and lower lip for VD at both 24 hours and 4-5 days, and for FCD at 24 hours.
  • Differences were also found between the two time-points for PPV at the lower lip.
  • The research found that the measurement reproducibility was good for all parameters for the lower lip, and was good for VD and FCD and fair to moderate for MFI and PPV for the upper lip.

Research Conclusion

  • The study concludes that utilizing SDF capillaroscopy to measure capillary microvasculature in newborn foals is feasible.
  • The lower lip was determined to provide the best measurement reproducibility.
  • The research suggests that further studies are necessary in foals with compromised cardiovascular health, especially those that are septic.

Cite This Article

APA
Freccero F, Di Maio C, Mariella J, Lanci A, Castagnetti C, Hallowell G. (2022). Assessment of the microvascular perfusion using sidestream dark-field imaging in healthy newborn foals. Vet Med Sci, 9(1), 158-166. https://doi.org/10.1002/vms3.1051

Publication

Veterinary medicine and science
ISSN: 2053-1095
NlmUniqueID: 101678837
Country: England
Language: English
Volume: 9
Issue: 1
Pages: 158-166

Researcher Affiliations

Freccero, Francesca
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO), Italy.
Di Maio, Chiara
  • Private Practitioner, Italy.
Mariella, Jole
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO), Italy.
Lanci, Aliai
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO), Italy.
Castagnetti, Carolina
  • Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO), Italy.
  • Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Bologna, Italy.
Hallowell, Gayle
  • IVC Evidensia, Pool House Equine Clinic, Lichfield, UK.

MeSH Terms

  • Animals
  • Horses
  • Animals, Newborn
  • Reproducibility of Results
  • Microcirculation
  • Perfusion / veterinary

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Citations

This article has been cited 1 times.
  1. Huang H, Zhao N, Zhu J, Li Q, Qiao Q, Yang Y, Zhou Y, Guo C, Guo Y. Antiangiogenic Medications Impede the Oral Mucosal Microcirculation and Interfere with Oral Wound Healing: A Complication Deserving of Attention. MedComm (2020) 2025 Jul;6(7):e70279.
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