Assessment of vitamin E concentrations in serum and cerebrospinal fluid of horses following oral administration of vitamin E.
Abstract: To determine concentrations of alpha-tocopherol in serum and CSF of healthy horses following administration of supplemental vitamin E in feed. Methods: 10 healthy adult horses. Methods: Horses were allocated to receive supplemental d-alpha-tocopherol (1,000 U/d [group A; n=5] or 10,000 U/d [group B; 5]) in feed for 10 days. Blood samples were collected before (baseline), during, and at intervals for 10 days after discontinuation of vitamin E administration for assessment of serum alpha-tocopherol concentration. Cerebrospinal fluid samples were collected prior to and 24 hours after cessation of vitamin E administration. Alpha-tocopherol concentrations in serum and CSF samples were analyzed via high-performance liquid chromatography; changes in those values during the treatment period were compared between groups, and the relationship of serum and CSF alpha-tocopherol concentrations was evaluated. Results: In both groups, serum alpha-tocopherol concentration increased significantly from baseline during vitamin E administration; values in group B were significantly greater than those in group A during and after treatment. At the end of vitamin E administration, CSF alpha-tocopherol concentration was not significantly greater than the baseline value in either group; however, the increase in CSF concentration was significant when the group data were combined and analyzed. Serum and CSF alpha-tocopherol concentrations were significantly correlated at baseline for all horses, but were not strongly correlated after 10 days of vitamin E administration. Conclusions: In healthy horses, daily oral administration of supplemental vitamin E in feed resulted in increases in serum and CSF alpha-tocopherol concentrations.
Publication Date: 2008-06-04 PubMed ID: 18518659DOI: 10.2460/ajvr.69.6.785Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study examines the effect of oral administration of vitamin E on its concentration levels in the blood and spinal fluid of healthy horses. It found that both blood and cerebrospinal fluid (CSF) concentration of alpha-tocopherol (a form of vitamin E) increased significantly with supplemental vitamin E in their diet.
Experimental Design and Methods
- The study was carried out on 10 healthy adult horses which were divided into two groups. One group (Group A) received a 1,000 U/daily dose of supplemental d-alpha-tocopherol (a form of vitamin E) while the other group (Group B) received a higher 10,000 U/daily dose.
- The supplemental vitamin E was administered to the horses’ feed for 10 days.
- Blood samples were collected from the horses before, during, and at intervals for 10 days after stopping the vitamin E administration. These samples were used to determine serum alpha-tocopherol concentration.
- Cerebrospinal fluid (CSF) samples were analyzed 24 hours after the end of the vitamin E administration to determine CSF alpha-tocopherol concentrations.
- The analysis of alpha-tocopherol concentrations was done via high-performance liquid chromatography.
Results and Analysis
- Results showed that the serum alpha-tocopherol concentration increased significantly from the baseline during the vitamin E administration in both groups. However, the increase was greater in Group B (that received a higher dose) than in Group A.
- The CSF alpha-tocopherol concentration at the end of the vitamin E administration wasn’t significantly greater than the baseline in either group. Nevertheless, when group data were combined and analyzed, the rise in CSF concentration was deemed significant.
- The research also found that at the beginning of the experiment, there was a significant correlation between serum and CSF alpha-tocopherol concentrations. But, such a correlation became weak after 10 days of vitamin E administration.
Conclusions
- The research concluded that daily oral administration of supplemental vitamin E in the feed of healthy horses led to increases in serum and CSF alpha-tocopherol concentrations.
- It’s implied that the quantity of the vitamin E supplement impacts the increase, with higher doses causing larger increases.
- However, the correlation between the vitamin E levels in the two body fluids seemed to reduce over time with vitamin E administration.
Cite This Article
APA
Higgins JK, Puschner B, Kass PH, Pusterla N.
(2008).
Assessment of vitamin E concentrations in serum and cerebrospinal fluid of horses following oral administration of vitamin E.
Am J Vet Res, 69(6), 785-790.
https://doi.org/10.2460/ajvr.69.6.785 Publication
Researcher Affiliations
- Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
MeSH Terms
- Administration, Oral
- Animals
- Dietary Supplements
- Female
- Horses / blood
- Horses / cerebrospinal fluid
- Male
- Random Allocation
- Statistics, Nonparametric
- Vitamin E / administration & dosage
- alpha-Tocopherol / blood
- alpha-Tocopherol / cerebrospinal fluid
Citations
This article has been cited 6 times.- Hales EN, Habib H, Favro G, Katzman S, Sakai RR, Marquardt S, Bordbari MH, Ming-Whitfield B, Peterson J, Dahlgren AR, Rivas V, Ramirez CA, Peng S, Donnelly CG, Dizmang BS, Kallenberg A, Grahn R, Miller AD, Woolard K, Moeller B, Puschner B, Finno CJ. Increased α-tocopherol metabolism in horses with equine neuroaxonal dystrophy.. J Vet Intern Med 2021 Sep;35(5):2473-2485.
- Finno CJ. Veterinary Pet Supplements and Nutraceuticals.. Nutr Today 2020 Mar-Apr;55(2):97-101.
- Burns EN, Finno CJ. Equine degenerative myeloencephalopathy: prevalence, impact, and management.. Vet Med (Auckl) 2018;9:63-67.
- Finno CJ, Bordbari MH, Gianino G, Ming-Whitfield B, Burns E, Merkel J, Britton M, Durbin-Johnson B, Sloma EA, McMackin M, Cortopassi G, Rivas V, Barro M, Tran CK, Gennity I, Habib H, Xu L, Puschner B, Miller AD. An innate immune response and altered nuclear receptor activation defines the spinal cord transcriptome during alpha-tocopherol deficiency in Ttpa-null mice.. Free Radic Biol Med 2018 May 20;120:289-302.
- Brown JC, Valberg SJ, Hogg M, Finno CJ. Effects of feeding two RRR-α-tocopherol formulations on serum, cerebrospinal fluid and muscle α-tocopherol concentrations in horses with subclinical vitamin E deficiency.. Equine Vet J 2017 Nov;49(6):753-758.
- Finno CJ, Estell KE, Katzman S, Winfield L, Rendahl A, Textor J, Bannasch DL, Puschner B. Blood and Cerebrospinal Fluid α-Tocopherol and Selenium Concentrations in Neonatal Foals with Neuroaxonal Dystrophy.. J Vet Intern Med 2015 Nov-Dec;29(6):1667-75.
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