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Association between myeloperoxidase concentration in equine frozen semen and post-thawing parameters.
Abstract: Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on the impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after cell lysis. It is responsible for the formation of hypochlorous acid, a strong oxidant agent, which could damage spermatozoa. The aim of this study was to determine the relation between MPO concentration and characteristics of frozen semen from stallions. Thirty-five straws from different stallions were analysed. Post-thawing spermatozoal concentration, and progressive and total motility were determined by Computer-Assisted Semen Analysis. Freezability was determined according to post-thawing progressive motility (above or below 15%). Percentage of alive spermatozoa and abnormal forms was determined after Eosin-Nigrosin and Diff-Quick(®) staining, respectively. Post-thawing MPO concentration was measured by enzyme-linked immunosorbent assay (ELISA). Our study shows that frozen thawed semen contains large amounts of free MPO. We also observed that post-thawing MPO ELISA assay can be used as an indicator of equine semen freezability. High MPO concentration samples showed lower total and progressive motility. A higher proportion of abnormal head shape associated with acrosome reaction was observed in our late examinations of the high concentration MPO group. Our results show that MPO adversely affects total and progressive motility of equine semen. A negative correlation between normal motile forms and MPO concentration was also observed. The effect of MPO on dead or abnormal forms remains to be precised.
© 2009 Blackwell Verlag GmbH.
Publication Date: 2009-04-15 PubMed ID: 19364394DOI: 10.1111/j.1439-0531.2009.01357.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research uncovers the correlation between a pro-oxidant enzyme, myeloperoxidase (MPO), and the quality of frozen semen in stallions, revealing that high concentrations of MPO negatively affect the post-thawing parameters of the semen.
Objectives of the Research
The study aimed to assess the impact of MPO, a potent enzyme contributing to oxidative damage, on the characteristics of frozen stallion semen following thawing. The objective was to:
- Measure the MPO concentration in post-thaw semen samples from different stallions.
- Analyze the correlation between MPO concentration and semen characteristics, including sperm concentration, total and progressive motility.
- Examine the relationship between MPO levels and sperm abnormalities.
Methodology
The researchers used an enzyme-linked immunosorbent assay (ELISA) to measure the MPO concentration in thirty-five different semen samples post-thawing. Other parameters examined were:
- Post-thawing spermatozoal concentration, and progressive and total motility, using computer-assisted semen analysis.
- Percentage of live spermatozoa and abnormal forms using Eosin-Nigrosin and Diff-Quick® staining.
- Freezability of semen, determined by post-thawing progressive motility.
Findings
The findings of the study suggest that the presence of high amounts of free MPO in thawed semen correlates with two key parameters.
- High MPO concentrations were associated with reduced total and progressive motility in the sperm samples. In other words, sperm in samples with more MPO were less able to move quickly and effectively.
- A high concentration of MPO was also linked to a greater proportion of sperm abnormalities, especially regarding head shape and acrosome reactions.
Conclusions
The study concluded that MPO could have a potential harmful effect on the total and progressive motility of horse semen, with a high concentration of MPO associated with poorer sperm quality. They also uncovered a clear negative correlation between normal motile forms and MPO concentration. However, the researchers stipulate that more work is still required to determine the exact effect of MPO on dead or abnormal sperm forms. This research provides valuable insights for equine reproduction and the ongoing issue of sub-optimal semen freezability.
Cite This Article
APA
Ponthier J, Franck T, Detilleux J, Mottart E, Serteyn D, Deleuze S.
(2009).
Association between myeloperoxidase concentration in equine frozen semen and post-thawing parameters.
Reprod Domest Anim, 45(5), 811-816.
https://doi.org/10.1111/j.1439-0531.2009.01357.x Publication
Researcher Affiliations
- Equine Clinic, Veterinary Medicine Faculty, ULg University of Liége, Liége, Belgium.
MeSH Terms
- Animals
- Freezing
- Horses / physiology
- Male
- Peroxidase / metabolism
- Semen / enzymology
- Semen Preservation / methods
- Semen Preservation / veterinary
Citations
This article has been cited 2 times.- Egyptien S, Deleuze S, Ledeck J, Ponthier J. Sperm Quality Assessment in Stallions: How to Choose Relevant Assays to Answer Clinical Questions. Animals (Basel) 2023 Oct 6;13(19).
- Ponthier J, Rigaux G, Parrilla-Hernandez S, Egyptien S, Gatez C, Carrasco Leroy C, Deleuze S. Case Report: Suppression of Harem Stallion Behavior and Fertility Following Anti-Gonadotropin-Releasing Hormone Vaccination of a Captive Wild Przewalski's Horse (Equus ferus przewalskii). Front Vet Sci 2020;7:569185.
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