Association of equine sperm population parameters with outcome of intracytoplasmic sperm injections.
Abstract: Limited clinical information is available regarding sperm population parameters that are important for use with equine intracytoplasmic sperm injection (ICSI). Therefore, the appropriateness of a sample of sperm is typically not known before ICSI. The aim of our study was to determine which sperm population characteristics were predictive of ICSI outcome. Frozen-thawed sperm samples (n = 114) from 37 stallions in a clinical program were analyzed after ICSI for percentages of normal morphology (MORPH+), live as assessed by eosin/nigrosin stain (LIVE+), membrane intact as assessed by hypoosmotic swelling test (HOS+), and DNA fragmentation determined by sperm chromatin dispersion (DNA-). ICSI was performed on 147 oocytes, and cleavage (≥2 cells), embryo development (morula or blastocyst), and pregnancy status after embryo transfer were determined. Among the examined sperm parameters, LIVE + correlated positively with MORPH+ and HOS+, and MORPH + negatively with DNA-; no other significant correlations were observed. When used for ICSI, sperm population percentages for MORPH+ and DNA- were not predictive of ICSI outcome, including cleavage, embryo development, and establishment of a pregnancy. Sperm population percentages significantly affecting ICSI outcomes were LIVE+ and HOS + for oocyte cleavage, LIVE + for embryo development, and HOS + for establishment of a pregnancy. The probability of a pregnancy was significantly higher for sperm populations having HOS+ ≥40% than populations having HOS+ ≤20%. The mean age of the donor mare per sperm-injected oocyte did not differ for oocyte cleavage, embryo production, or establishment of pregnancy. In our study, the probability of sperm-injected oocytes to develop into an embryo (morula or blastocyst) improved when sperm were selected from a population with higher indicators of membrane integrity (LIVE+ and HOS+).
Copyright © 2018. Published by Elsevier Inc.
Publication Date: 2018-07-05 PubMed ID: 30006126DOI: 10.1016/j.theriogenology.2018.06.027Google Scholar: Lookup
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- Journal Article
Summary
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The research paper explores the correlation between different characteristics of equine (horse) sperm and the success rate of a fertility procedure called intracytoplasmic sperm injection (ICSI). The study finds that the liveliness and membrane integrity of the sperm play significant roles in the success of the procedure and subsequent embryo development and establishment of pregnancy.
Introduction and Aim of the Study
- The paper starts by noting the lack of clinical information available about important sperm parameters for successful equine ICSI procedures.
- The objective of the study was to identify sperm characteristics that could predict the outcome of an ICSI procedure.
Materials and Methods
- Sperm samples from 37 stallions were frozen, thawed, and then analyzed.
- The sperm samples were tested for normal morphology (MORPH+), vitality as determined by eosin/nigrosin stain (LIVE+), membrane integrity using hypoosmotic swelling test (HOS+), and the level of DNA fragmentation (DNA-).
- ICSI was performed on 147 oocytes and the progress of cleavage, embryo development, and pregnancy status after embryo transfer were observed.
Results
- The results showed a positive correlation between LIVE+, MORPH+ and HOS+, while MORPH+ negatively correlated with DNA-.
- MORPH+ and DNA- percentages were not predictors of ICSI outcomes such as oocyte cleavage, embryo development, and establishment of a pregnancy.
- LIVE+ and HOS+ percentages, however, significantly affected ICSI outcomes, specifically oocyte cleavage and embryo development, with HOS+ also affecting the establishment of pregnancy.
- A higher probability of pregnancy was observed for sperm populations with HOS+ values higher or equal to 40%, compared to populations with HOS+ values less than or equal to 20%.
Conclusions
- The age of the donor mare had no significant difference for oocyte cleavage, embryo production or establishment of pregnancy.
- The study concludes that the chances of successful embryo development following ICSI improves when sperm are selected from a population with higher integrity indicators (LIVE+ and HOS+).
In essence, this research brings valuable insights into the equine ICSI process, highlighting the importance of sperm vitality and membrane integrity on the procedure’s success rate.
Cite This Article
APA
Gonzalez-Castro RA, Carnevale EM.
(2018).
Association of equine sperm population parameters with outcome of intracytoplasmic sperm injections.
Theriogenology, 119, 114-120.
https://doi.org/10.1016/j.theriogenology.2018.06.027 Publication
Researcher Affiliations
- Department of Biomedical Sciences, Colorado State University, Equine Reproduction Laboratory, 3101 Rampart Road, Fort Collins, CO, 80521, USA.
- Department of Biomedical Sciences, Colorado State University, Equine Reproduction Laboratory, 3101 Rampart Road, Fort Collins, CO, 80521, USA. Electronic address: Elaine.Carnevale@colostate.edu.
MeSH Terms
- Animals
- Embryo Culture Techniques / veterinary
- Embryo Transfer
- Embryonic Development
- Female
- Horses
- Male
- Pregnancy
- Sperm Injections, Intracytoplasmic / veterinary
- Spermatozoa / physiology
Citations
This article has been cited 3 times.- Catandi GD, LiPuma L, Obeidat YM, Maclellan LJ, Broeckling CD, Chen T, Chicco AJ, Carnevale EM. Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares. Reproduction 2022 Apr 1;163(4):183-198.
- Gimeno BF, Bariani MV, Laiz-Quiroga L, Martínez-León E, Von-Meyeren M, Rey O, Mutto AÁ, Osycka-Salut CE. Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status. Animals (Basel) 2021 Jan 3;11(1).
- Ribas-Maynou J, Yeste M, Salas-Huetos A. The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals. Biology (Basel) 2020 Jul 21;9(7).
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