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Molecular biology reports2019; 46(4); 4453-4461; doi: 10.1007/s11033-019-04900-w

Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta.

Abstract: The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (p < 0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.
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Publication Date: 2019-06-07 PubMed ID: 31175514DOI: 10.1007/s11033-019-04900-wGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the possible association between specific gene variants in horses from the Danube delta region in Romania and their resistance or lack of resistance to the West Nile virus. It was discovered that horses with specific genetic markers in the SLC11A1 and TLR4 genes were most likely to have an immunity to the virus. Further research is required to understand the implication of these findings.

Research Background

  • The West Nile virus (WNV) is a disease carried by mosquitoes that can cause severe inflammation of the brain and spinal cord in both humans and animals.
  • Horses, due to their specific susceptibility to the WNV infection, serve as a sentinel species to study and anticipate the spread of the virus.

Who and What was Studied

  • A population of semi-wild Romanian horses living in the Danube delta region was used for this study.
  • Genetic polymorphisms – variations in the DNA sequence – were analyzed to determine any associations with resistance to the West Nile virus.
  • Thirty-six SNPs (Single Nucleotide Polymorphisms) located in 28 immunity-related genes and 26 microsatellites located in the Major Histocompatibility Complex (MHC) and LY49 complex genomic regions were compared among 57 horses with the WNV virus and 32 without.

Study Results

  • The most significant association was found between horses with specific genetic markers in the SLC11A1 and TLR4 genes and resistance to the West Nile virus.
  • Markers from five other immune system-related genes (ADAM17, CXCR3, IL12A, MAVS, TNFA) along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status, but to a lesser extent.
  • The OAS1 gene, previously associated with severity of disease from the West Nile virus, did not demonstrate any significant association with resistance to the disease among this population of horses.

Study Significance

  • This research provides initial understanding of specific genetic factors that may contribute to the resistance of horses to the West Nile virus.
  • The findings may ultimately contribute to understanding the susceptibility to the West Nile virus in other species, including humans.
  • Further research, however, is required to fully comprehend the evident correlations and their potential application in genetic selection or therapeutic interventions.

Cite This Article

APA
Stejskalova K, Janova E, Horecky C, Horecka E, Vaclavek P, Hubalek Z, Relling K, Cvanova M, D'Amico G, Mihalca AD, Modry D, Knoll A, Horin P. (2019). Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta. Mol Biol Rep, 46(4), 4453-4461. https://doi.org/10.1007/s11033-019-04900-w

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 46
Issue: 4
Pages: 4453-4461

Researcher Affiliations

Stejskalova, K
  • Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic.
Janova, E
  • Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic.
  • CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic.
Horecky, C
  • Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.
  • CEITEC-MENDELU, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.
Horecka, E
  • Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.
  • CEITEC-MENDELU, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.
Vaclavek, P
  • SVU Jihlava, Rantirovska 93/20, Horni Kosov, 58601, Jihlava, Czech Republic.
Hubalek, Z
  • Institute of Vertebrate Biology of the Academy of Sciences, Květná 8, 60365, Brno, Czech Republic.
Relling, K
  • Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1, 612 42, Brno, Czech Republic.
Cvanova, M
  • Faculty of Medicine, Institute of Biostatistics and Analyses, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
D'Amico, G
  • Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400362, Cluj-Napoca, Romania.
Mihalca, A D
  • Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400362, Cluj-Napoca, Romania.
Modry, D
  • CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic.
  • Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1, 612 42, Brno, Czech Republic.
  • Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice, 370 05, Czech Republic.
Knoll, A
  • Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.
  • CEITEC-MENDELU, Mendel University in Brno, Zemědělská 1/1665, 613 00, Brno, Czech Republic.
Horin, P
  • Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic. horin@dior.ics.muni.cz.
  • CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Palackeho 1, 61242, Brno, Czech Republic. horin@dior.ics.muni.cz.

MeSH Terms

  • Animals
  • Antibodies, Viral / analysis
  • Antibodies, Viral / blood
  • Enzyme-Linked Immunosorbent Assay
  • Horses / blood
  • Horses / genetics
  • Horses / immunology
  • Microsatellite Repeats / genetics
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide / genetics
  • Romania
  • Sentinel Species
  • West Nile Fever / genetics
  • West Nile Fever / immunology
  • West Nile Fever / veterinary
  • West Nile virus / pathogenicity

Grant Funding

  • CZ.1.05/1.1.00/02 / Central European Institute of Technology
  • NPU LQ1601 / Ministerstvo u0160kolstvu00ed, Mlu00e1deu017ee a Tu011blovu00fdchovy

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Citations

This article has been cited 3 times.
  1. Stejskalova K, Vychodilova L, Janova E, Oppelt J, Horin P. Innate Immunity Toll-Like Triad TLR6-1-10 and Its Diversity in Distinct Horse Breeds. Vet Med Sci 2025 Mar;11(2):e70230.
    doi: 10.1002/vms3.70230pubmed: 39918481google scholar: lookup
  2. Dinu S, Stancu IG, Cotar AI, Ceianu CS, Pintilie GV, Karpathakis I, Fălcuță E, Csutak O, Prioteasa FL. Continuous and Dynamic Circulation of West Nile Virus in Mosquito Populations in Bucharest Area, Romania, 2017-2023. Microorganisms 2024 Oct 17;12(10).
    doi: 10.3390/microorganisms12102080pubmed: 39458389google scholar: lookup
  3. Stejskalova K, Janova E, Splichalova P, Futas J, Oppelt J, Vodicka R, Horin P. Twelve toll-like receptor (TLR) genes in the family Equidae - comparative genomics, selection and evolution. Vet Res Commun 2024 Apr;48(2):725-741.
    doi: 10.1007/s11259-023-10245-4pubmed: 37874499google scholar: lookup
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