Auditory enrichment on facial and physiological responses of Pantaneiro geldings and mares under short-term stress.
Abstract: This study aimed to evaluate the effect of auditory stimuli on mares and geldings during social isolation and movement restriction, and their ability to promote the reduction of stress responses. The research was conducted with eight Pantaneiro horses, divided into Experiment I (4 mares) and Experiment II (4 geldings), both experiments were executed equally using a 4x4 Latin square design with experimental (classical, country and new age) and control (no music) treatments. Physiological parameters (heart rate, heart rate variability, respiratory rate, ocular temperature by infrared thermography, and surface temperature by infrared thermometer) and facial expressions (eye closure (AU143), blink (AU145), half blink (AU47), inner brow raiser (AU101), eye white increase (AD1), tongue show (AD19), nostril dilator (AD38), chewing (AD81), ears forward (EAD101), and ear rotator (EAD104)) were assessed throughout the 24 minutes that the animals remained in the restraining stock. In Experiment I, there was a reduction (p < 0.05) in heart rate during classical and country treatments and an increase (p < 0.05) in auricular and body temperatures during the classical treatment. Additionally, the frequencies of expressions of nostril dilator (AD38), ear rotator (EAD104), and half blink (AU47) were lower (p < 0.05). In Experiment II, there was an increase (p < 0.05) in ocular temperature during the country and control treatments, and a higher frequency (p < 0.05) of ears forward (EAD101) during the country treatment. Exposure to Beethoven's 9th Symphony (classical genre) and Hank Williams Jr.'s "Ramblin' In My Shoes" (country genre) reduced stress in mares. Geldings showed less pronounced responses to music genres, indicating a possible preference for Janet Marlow's "Horsing Around" (new age genre). Music can be used as auditory enrichment for horses in the specific context of this study.
Copyright: © 2025 da Fé et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publication Date: 2025-05-20 PubMed ID: 40392914DOI: 10.1371/journal.pone.0323649Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study investigates how different types of music affect the stress levels of Pantaneiro horses when they’re socially isolated or movement-restricted. It suggests that playing music, especially classical and country pieces, may help lower stress responses in these horses.
Key Objectives of the Research
- The researchers were interested in understanding how auditory stimulation could influence stress responses in Pantaneiro horses. The horses were put under conditions of social isolation and movement restriction, context in which stress levels are typically elevated.
Methodology
- The study involved eight Pantaneiro horses, with four mares (female horses) participating in the first experiment and four geldings (castrated male horses) in the second.
- Both experiments followed a 4×4 Latin square design, a statistical method used in experiments to minimize the influence of extraneous factors.
- Each group of horses was exposed to different types of music – classical, country, and new age – and a control group was maintained without any music.
- During each 24-minute session when the horses were in the restraining stock (a device that keeps them in a fixed position), various physiological parameters and facial expressions were measured. These included heart rate, respiratory rate, eye closure, blink and half blink, ear rotation, nostril dilation, and others.
Findings
- In the first experiment with mares, the researchers noticed a significant drop in heart rate when classical and country music were played. The mares also showed increased body and auricular (ear) temperatures under classical music, and reduced frequency of certain facial expressions.
- In the second experiment with geldings, there was a significant increase in ocular (eye) temperature during the country and control treatments, and a higher frequency of ears-forward expression under country music. The geldings showed less pronounced responses to music compared to mares, indicating a possible preference for new age music.
- Notably, the researchers found that playing Beethoven’s 9th Symphony (classical genre) and Hank Williams Jr.’s “Ramblin’ In My Shoes” (country genre) led to reduced stress in mares. However, the specific songs might not have the same effects in a broader population of horses.
Study Implications
- The study indicates that auditory stimuli, particularly certain genres of music, can be effective in alleviating stress in Pantaneiro horses. This could be beneficial during situations of social isolation or when movement is restricted.
- While the study is limited in its sample size and the specific horse breed, its findings suggest that further research could be useful in understanding how to better manage the stress levels of horses in different situations.
Cite This Article
APA
da Fé VCS, Dos Santos VMO, de Lima ACB, Hernandes MSP, Caldara FR, Gomes MNB.
(2025).
Auditory enrichment on facial and physiological responses of Pantaneiro geldings and mares under short-term stress.
PLoS One, 20(5), e0323649.
https://doi.org/10.1371/journal.pone.0323649 Publication
Researcher Affiliations
- Graduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
- Graduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
- Graduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
- Graduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
- Graduate Program in Animal Science, Faculty of Agricultural Sciences, Federal University of Grande Dourados, Dourados, Brazil.
- Graduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
MeSH Terms
- Animals
- Horses / physiology
- Female
- Heart Rate / physiology
- Acoustic Stimulation
- Body Temperature / physiology
- Facial Expression
- Stress, Psychological / physiopathology
- Stress, Physiological
- Male
- Respiratory Rate / physiology
Conflict of Interest Statement
The authors have declared that no competing interests exist.
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists