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Home / Equine Research Bank / Front Vet Sci / Basal and inducible Osterix expression reflect equine mesenc...
Frontiers in veterinary science2023; 10; 1125893; doi: 10.3389/fvets.2023.1125893

Basal and inducible Osterix expression reflect equine mesenchymal progenitor cell osteogenic capacity.

Abstract: Mesenchymal stem cells are characterized by their capacities for extensive proliferation through multiple passages and, classically, tri-lineage differentiation along osteogenic, chondrogenic and adipogenic lineages. This study was carried out to compare osteogenesis in equine bone marrow-, synovium- and adipose-derived cells, and to determine whether osteogenic capacity is reflected in the basal expression of the critical osteogenic transcription factors Runx2 and Osterix. Unassigned: Bone marrow, synovium and adipose tissue was collected from six healthy 2-year-old horses. Cells were isolated from these sources and expanded through two passages. Basal expression of Runx2 and Osterix was assessed in undifferentiated third passage cells, along with their response to osteogenic culture conditions. Unassigned: Bone marrow-derived cells had significantly higher basal expression of Osterix, but not Runx2. In osteogenic medium, bone-marrow cells rapidly developed dense, multicellular aggregates that stained strongly for mineral and alkaline phosphatase activity. Synovial and adipose cell cultures showed far less matrix mineralization. Bone marrow cells significantly up-regulated alkaline phosphatase mRNA expression and enzymatic activity at 7 and 14 days. Alkaline phosphatase expression and activity were increased in adipose cultures after 14 days, although these values were less than in bone marrow cultures. There was no change in alkaline phosphatase in synovial cultures. In osteogenic medium, bone marrow cultures increased both Runx2 and Osterix mRNA expression significantly at 7 and 14 days. Expression of both transcription factors did not change in synovial or adipose cultures. Unassigned: These results demonstrate that basal Osterix expression differs significantly in progenitor cells derived from different tissue sources and reflects the osteogenic potential of the cell populations.
Copyright © 2023 Andrietti, Durgam, Naumann and Stewart.
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Publication Date: 2023-03-23 PubMed ID: 37035801PubMed Central: PMC10076790DOI: 10.3389/fvets.2023.1125893Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the osteogenesis (bone-formation) capabilities of stem cells procured from various parts of a horse’s body. The study found that the basal expression of the osteogenic transcription factor, Osterix, in these cells can help depict their potential for bone-formation.

Research Methodology

  • The researchers collected bone marrow, synovium, and adipose tissue from six healthy 2-year-old horses.
  • Cells were isolated from these tissues and cultivated through two passages.
  • The scientists assessed the basal expression of Runx2 and Osterix—critical osteogenic transcription factors—in these third passage cells. They also observed these cells’ response to osteogenic culture conditions.

Findings

  • The bone marrow-derived cells showed significantly higher basal expression of Osterix, but not Runx2
  • When placed in osteogenic medium, bone-marrow cells rapidly formed dense, multicellular aggregates that stained strongly for mineral and alkaline phosphatase activity.
  • In comparison, synovial and adipose cell cultures showed less matrix mineralization.
  • At day 7 and 14, bone marrow cells noticeably up-regulated the expression and enzymatic activity of alkaline phosphatase.
  • After 14 days, alkaline phosphatase expression and activity were also found increased in adipose cultures, but they were lesser than in bone marrow cultures. There was no change in synovial cultures.
  • In the osteogenic medium, bone marrow cultures showed a significant increase in the mRNA expression of both Runx2 and Osterix at 7 and 14 days. This expression did not change in synovial or adipose cultures.

Conclusions

  • The study concluded that the basal expression of Osterix significantly varies in progenitor cells derived from different tissue sources.
  • This attribute correlates with the osteogenic potential (potential for bone formation) of these cell populations.

Cite This Article

APA
Andrietti ALP, Durgam SS, Naumann B, Stewart M. (2023). Basal and inducible Osterix expression reflect equine mesenchymal progenitor cell osteogenic capacity. Front Vet Sci, 10, 1125893. https://doi.org/10.3389/fvets.2023.1125893

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 10
Pages: 1125893
PII: 1125893

Researcher Affiliations

Andrietti, Antonella Liza Pantaleoni
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL, United States.
Durgam, Sushmitha S
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL, United States.
Naumann, Brittany
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL, United States.
Stewart, Matthew
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL, United States.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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