Bioactive glass ceramic nanoparticles-coated poly(l-lactic acid) scaffold improved osteogenic differentiation of adipose stem cells in equine.
Abstract: Horses with big bone fractures have low chance to live mainly due to the lake of a proper treatment strategy. We believe that further attempts in equine bone tissue engineering will probably be required to meet all the needs for the lesion therapies. Therefore in this study we aimed to investigate the osteogenic differentiation capacity of equine adipose-derived stem cells (e-ASCs) on nano-bioactive glass (nBGs) coated poly(l-lactic acid) (PLLA) nanofibers scaffold (nBG-PLLA). Using electrospinning technique, PLLA scaffold was prepared successfully and coated with nBGs. Fabricated nanofibers were characterized by MTT, SEM, and FTIR analyses, and then osteogenic differentiation potential of isolated e-ASCs was investigated by the most key osteogenic markers, namely Alizarin red-S, ALP, calcium content and bone related (RUNX2, Collagen I, Osteonectin, and ALP) gene markers. Our results indicated that nBGs was successfully coated on PLLA scaffold and this scaffold had no negative (p>0.05) effect on cell growth rate as indicated by MTT assay. Moreover, e-ASCs that differentiated on nBGs-PLLA scaffold showed a higher (p<0.05) ALP activity, more (p<0.05) calcium content, and higher (p<0.05) expression of bone-related genes than that on uncoated PLLA scaffold and TCPS. According to the results, a combination of bioceramics and biopolymeric nanofibers hold valuable promising potentials to use for bone tissue engineering application and regenerative medicine.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Publication Date: 2017-07-20 PubMed ID: 28851519DOI: 10.1016/j.tice.2017.07.003Google Scholar: Lookup
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Summary
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This study explores how the osteogenic differentiation capacity of equine adipose-derived stem cells (e-ASCs) can be enhanced by coating a Poly(L-Lactic Acid) (PLLA) nanofibre scaffold with nano-bioactive glass (nBGs). The research indicates that this combined approach may hold significant potential for applications in bone tissue engineering and regenerative medicine.
Research Methodology
- The team experimented with equine stem cells derived from adipose tissue, a byproduct of many plastic surgery procedures.
- They prepared a scaffold using Poly(L-Lactic Acid) (PLLA) using the electrospinning technique.
- This scaffold was then coated with nano-bioactive glass (nBGs) and was characterized using MTT, SEM, and FTIR analyses.
- The osteogenic differentiation potential of isolated e-ASCs was examined by key osteogenic markers, including Alizarin red-S, ALP, calcium content, and bone-related gene markers (RUNX2, Collagen I, Osteonectin, ALP).
Results and Findings
- The results indicated successful coating of the PLLA scaffold with nBGs. The team confirmed that this coating had no negative impact (p>0.05) on cell growth rate (as indicated by the MTT assay).
- The stem cells that differentiated on the nBG-PLLA scaffold showed increased ALP activity, more calcium content, and higher expression of bone-related genes than on both the uncoated PLLA scaffold and Tissue Culture Polystyrene (TCPS).
- The results support the notion that the combination of bioceramics and biopolymeric nanofibers can be used effectively in bone tissue engineering applications and regenerative medicine.
Conclusion
This study provides a promising new approach for healing large bone fractures in horses, which are often fatal due to the lack of effective treatment strategies. Future research on equine bone tissue engineering could boost the development of new therapies for lesions by focusing on the combination of bioceramics with biopolymeric nanofibers.
Cite This Article
APA
Mahdavi FS, Salehi A, Seyedjafari E, Mohammadi-Sangcheshmeh A, Ardeshirylajimi A.
(2017).
Bioactive glass ceramic nanoparticles-coated poly(l-lactic acid) scaffold improved osteogenic differentiation of adipose stem cells in equine.
Tissue Cell, 49(5), 565-572.
https://doi.org/10.1016/j.tice.2017.07.003 Publication
Researcher Affiliations
- Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
- Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran. Electronic address: arsalehi@ut.ac.ir.
- Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
- Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
- Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
MeSH Terms
- Adipocytes / cytology
- Animals
- Cell Differentiation
- Cell Proliferation
- Ceramics
- Horses
- Mesenchymal Stem Cells / cytology
- Nanoparticles / chemistry
- Osteogenesis
- Polyesters
- Tissue Engineering / methods
- Tissue Scaffolds / chemistry
Citations
This article has been cited 7 times.- Geevarghese R, Sajjadi SS, Hudecki A, Sajjadi S, Jalal NR, Madrakian T, Ahmadi M, Włodarczyk-Biegun MK, Ghavami S, Likus W, Siemianowicz K, Łos MJ. Biodegradable and Non-Biodegradable Biomaterials and Their Effect on Cell Differentiation. Int J Mol Sci 2022 Dec 19;23(24).
- Tan T, Song D, Hu S, Li X, Li M, Wang L, Feng H. Structure and Properties of Bioactive Glass-Modified Calcium Phosphate/Calcium Sulfate Biphasic Porous Self-Curing Bone Repair Materials and Preliminary Research on Their Osteogenic Effect. Materials (Basel) 2022 Nov 8;15(22).
- Merlo B, Baldassarro VA, Flagelli A, Marcoccia R, Giraldi V, Focarete ML, Giacomini D, Iacono E. Peptide Mediated Adhesion to Beta-Lactam Ring of Equine Mesenchymal Stem Cells: A Pilot Study. Animals (Basel) 2022 Mar 15;12(6).
- Thyparambil NJ, Gutgesell LC, Bromet BA, Flowers LE, Greaney S, Day DE, Semon JA. Bioactive borate glass triggers phenotypic changes in adipose stem cells. J Mater Sci Mater Med 2020 Mar 23;31(4):35.
- García-Sánchez D, Fernández D, Rodríguez-Rey JC, Pérez-Campo FM. Enhancing survival, engraftment, and osteogenic potential of mesenchymal stem cells. World J Stem Cells 2019 Oct 26;11(10):748-763.
- Incesoy MA, Sarikas M, Kaya HB, Yavuz AD, Sulak B, Ozdemir AA, Ozarslan AC, Uzer G, Yıldız F, Yucel S, Gurkan V. Bioactive Glass Graft vs Allograft in Benign Bone Lesions: A Retrospective Comparative Study. HSS J 2025 Mar 20;:15563316251321825.
- Ferreira-Baptista C, Ferreira R, Fernandes MH, Gomes PS, Colaço B. Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells' Biological Profile and Osteogenic Potential in Companion Animals. Vet Sci 2023 Nov 24;10(12).
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