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Home / Equine Research Bank / J Virol / Biological characterization of Rev variation in equine infec...
Journal of virology1998; 72(5); 4421-4426; doi: 10.1128/JVI.72.5.4421-4426.1998

Biological characterization of Rev variation in equine infectious anemia virus.

Abstract: Sequence analysis identified significant variation in the second exon of equine infectious anemia virus (EIAV) rev. Functional analysis indicated that limited amino acid variation in Rev significantly altered the export activity of the protein but did not affect Rev-dependent alternative splicing. EIAV Rev can mediate export through two independent cis-acting Rev-responsive elements (RREs), and differences among Rev variants were more pronounced when both RREs were present. Variation in Rev may be an important mechanism for regulation of virus replication in vivo and may contribute to changes in clinical disease.
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Publication Date: 1998-04-29 PubMed ID: 9557734PubMed Central: PMC109674DOI: 10.1128/JVI.72.5.4421-4426.1998Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The article reveals the significant variations found in the second exon of the equine infectious anemia virus (EIAV) rev. These variations can notably impact the export activity of the protein without affecting the rev-dependent alternative splicing. The paper further posits that the observed differences in the Rev variants may contribute to changes in the clinical disease and can regulate virus replication.

Detailed Explanation

  • The research revolves around the sequence analysis of the equine infectious anemia virus (EIAV) rev. EIAV is a retrovirus that infects horses, similar to the human immunodeficiency virus (HIV) in humans. The rev gene is crucial in the life cycle of retroviruses, as it regulates the export of viral RNA from the nucleus to the cytoplasm of the host cells.
  • The researchers have identified significant variation in the second exon of the rev gene. An exon is a portion of a gene that codes for proteins. Thus, changes in exons have the potential to alter the protein structure and function drastically.
  • Functionally, it was observed that even limited variation in the amino acids making up the Rev protein could notably alter the export capabilities of the protein. Varying the structure of the protein can change the way it interacts with other molecules, leading to changes in functionality. This makes such variations a potential interest in understanding and combating the virus.
  • Interestingly, despite the changes in export activity, these identified Rev variations did not affect rev-dependent alternative splicing. Alternative splicing is a mechanism whereby different protein variants are produced from the same gene, contributing significantly to protein diversity and functional complexity within the cell.
  • The study notes that EIAV Rev can mediate export through two independent cis-acting Rev-responsive elements (RREs). An RRE is a specific RNA sequence to which the Rev protein binds. Differences among Rev variants were more pronounced when both RREs were present. This finding shows that the Rev protein interaction with the RREs, critical in Rev’s role in the virus’ life cycle, is impacted by the Rev variations.
  • Lastly, the authors suggest that variation in Rev may be an influential mechanism for the regulation of virus replication in vivo (inside the organism). By changing how the Rev protein functions, the virus may alter how quickly or easily it can replicate inside the host organism. These variations may also contribute to changes in clinical disease, impacting how the disease manifests and progresses within its host.

Cite This Article

APA
Belshan M, Harris ME, Shoemaker AE, Hope TJ, Carpenter S. (1998). Biological characterization of Rev variation in equine infectious anemia virus. J Virol, 72(5), 4421-4426. https://doi.org/10.1128/JVI.72.5.4421-4426.1998

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 72
Issue: 5
Pages: 4421-4426

Researcher Affiliations

Belshan, M
  • Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50011, USA.
Harris, M E
    Shoemaker, A E
      Hope, T J
        Carpenter, S

          MeSH Terms

          • Alternative Splicing
          • Amino Acid Sequence
          • Animals
          • Equidae
          • Gene Products, rev / genetics
          • Gene Products, rev / physiology
          • Genetic Variation
          • Infectious Anemia Virus, Equine / genetics
          • Molecular Sequence Data
          • RNA, Viral

          Grant Funding

          • AI35477 / NIAID NIH HHS

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          Citations

          This article has been cited 25 times.
          1. Jackson PEH, Holsey J, Turse L, Hammarskjold ML, Rekosh D. Rev-Rev Response Element Activity Selection Bias at the Human Immunodeficiency Virus Transmission Bottleneck.. Open Forum Infect Dis 2023 Oct;10(10):ofad486.
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