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Home / Equine Research Bank / Acta Vet Scand / Blood pressure in Warmblood horses before and during a eugly...
Acta veterinaria Scandinavica2016; 58(Suppl 1); 65; doi: 10.1186/s13028-016-0247-y

Blood pressure in Warmblood horses before and during a euglycemic-hyperinsulinemic clamp.

Abstract: Insulin resistance (IR) in humans is related to hypertension and impaired vasodilation. Insulin administration has been shown to lower blood pressure both in insulin resistant as well as in insulin sensitive individuals. The aim of the study was to investigate the association between insulin sensitivity and alterations in blood pressure in healthy horses before and after a euglycemic-hyperinsulinemic clamp (EHC). A 3-h EHC was performed in 13 healthy horses (11 mares, 2 geldings). Blood samples for measurement of plasma glucose and insulin were collected before the start of the EHC, every 10 min during the EHC and immediately after the EHC. Mean, systolic- and diastolic blood pressure was measured before and during the final 10 min of the EHC using an indirect high-definition oscillometric monitor (HDO, horse model) applied to the middle of the coccygeal artery. Five consecutive measurements were made in each horse and on each occasion. Insulin and glucose data from the EHC were used to calculate the mean rate of glucose disposal per unit of insulin during steady state (M/I ratio). Insulin resistance was defined as a M/I ratio <5 mg/kg/min/mUL (Lindåse et al. in Am J Vet Res 77:300-309, 2016). Results: Insulin administration decreased systolic, diastolic and mean arterial pressure in all horses. The M/I ratio for all horses was negatively correlated with the decrease in systolic blood pressure (r = 0.55, P = 0.004) and mean arterial pressure (r = 0.31, P = 0.048) but not diastolic blood pressure (r = 0.12, P = 0.26). Eight horses were defined as insulin resistant (IR) and five horses had normal insulin sensitivity. The five horses with normal insulin sensitivity showed a greater decrease in systolic blood pressure (-17.0 ± 7.4 vs. -3.4 ± 4.6 mmHg, P = 0.001) and MAP (19.2 ± 14.7 vs. 6.9 ± 8.7 mmHg, P = 0.04) than IR horses. There was no difference in the decrease in diastolic blood pressure between groups (16 ± 12.8 vs. 8.9 ± 12.1 mmHg, P = 0.17). Conclusions: This study indicates that there is a relationship between insulin sensitivity and systolic and MAP in horses. However, studies on a larger number of horses are needed to confirm this association.
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Publication Date: 2016-10-20 PubMed ID: 27766986PubMed Central: PMC5073943DOI: 10.1186/s13028-016-0247-yGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on analyzing the connection between insulin sensitivity and alterations in blood pressure in healthy horses, using a euglycemic-hyperinsulinemic clamp (EHC) process.

Research Method

  • A euglycemic-hyperinsulinemic clamp (EHC) was performed on 13 healthy horses. This method involved maintaining their blood glucose levels while raising the insulin levels.
  • Blood samples were gathered at specific intervals to measure both plasma glucose and insulin levels.
  • A high-definition oscillometric monitor was used for measuring the systolic, diastolic, and mean arterial pressure of the horses both before and during the EHC test.
  • The M/I ratio, representing the mean rate of glucose disposal per insulin unit during steady state, was calculated from the collected insulin and glucose data.
  • Horses with M/I ratio less than 5 mg/kg/min/mUL were categorized as insulin-resistant (IR), as per the set criterion.

Key Findings

  • The administration of insulin resulted in decreased systolic, diastolic, and mean arterial pressure in all horses.
  • A negative correlation was found between the M/I ratio and the decrease in systolic blood pressure (r = 0.55, P = 0.004) and mean arterial pressure (r = 0.31, P = 0.048). This suggests that as insulin sensitivity decreased, the blood pressure tended to decrease less.
  • No significant correlation was observed between M/I ratios and diastolic blood pressure changes.
  • Of the 13 horses, eight were classified as insulin-resistant and five as insulin-sensitive. The insulin-sensitive horses showed a more significant decrease in systolic blood pressure and mean arterial pressure than the insulin-resistant ones.

Conclusion

  • This research indicates a potential connection between insulin sensitivity and fluctuations in the systolic and mean arterial pressure in horses.
  • However, the researchers recommend further studies with a larger sample size to conclusively establish this association.

Cite This Article

APA
Nostell KE, Lindåse SS, Bröjer JT. (2016). Blood pressure in Warmblood horses before and during a euglycemic-hyperinsulinemic clamp. Acta Vet Scand, 58(Suppl 1), 65. https://doi.org/10.1186/s13028-016-0247-y

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 58
Issue: Suppl 1
Pages: 65
PII: 65

Researcher Affiliations

Nostell, Katarina E A
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 750 07, Uppsala, Sweden. Katarina.Nostell@slu.se.
Lindåse, Sanna S
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 750 07, Uppsala, Sweden.
Bröjer, Johan T
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 750 07, Uppsala, Sweden.

MeSH Terms

  • Animals
  • Blood Glucose / analysis
  • Blood Glucose / drug effects
  • Blood Pressure / drug effects
  • Blood Pressure / physiology
  • Female
  • Glucose Clamp Technique / veterinary
  • Horses / physiology
  • Insulin / blood
  • Insulin / pharmacology
  • Insulin Resistance
  • Male

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Citations

This article has been cited 2 times.
  1. D' Fonseca NMM, Beukers M, Wijnberg ID, Navas de Solis C, de Ruijter-Villani M, van Doorn DA, Stout TAE, Roelfsema E. Effect of a long-term high-energy diet on cardiovascular parameters in Shetland pony mares. J Vet Intern Med 2021 Sep;35(5):2427-2436.
    doi: 10.1111/jvim.16229pubmed: 34350640google scholar: lookup
  2. da Silva AA, do Carmo JM, Li X, Wang Z, Mouton AJ, Hall JE. Role of Hyperinsulinemia and Insulin Resistance in Hypertension: Metabolic Syndrome Revisited. Can J Cardiol 2020 May;36(5):671-682.
    doi: 10.1016/j.cjca.2020.02.066pubmed: 32389340google scholar: lookup
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