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Proceedings of the National Academy of Sciences of the United States of America1977; 74(2); 524-528; doi: 10.1073/pnas.74.2.524

Bovine papilloma virus: presence of virus-specific DNA sequences in naturally occurring equine tumors.

Abstract: Four of five spontaneous benign equine connective tissue tumors of unknown etiology and a bovine papilloma virus (BPV)-induced equine tumor contained BPV-specific DNA sequences as determined by DNA-DNA hybridization of DNA from tumors with BPV DNA labeled in vitro. Analysis of the kinetics of reassociation indicated that 20-75% of the BPV genome was present in the various tumors. The number of partial BPV genome equivalents ranged from 60 to 500 copies per diploid quantity of cellular DNA. Thermal denaturation profiles of duplexes formed between labeled BPV DNA and DNA from tumor cells indicated two tumors contained viral DNA with base sequences identical to BPV DNA. Three tumors (including DNA from the BPV-induced tumor) contained BPV-related DNA sequences that were less thermally stable. The decrease in thermal denaturation temperature may be due to the presence of (adenine + thymine)-rich regions of the BPV genome in the tumor cells.
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Publication Date: 1977-02-01 PubMed ID: 191813PubMed Central: PMC392322DOI: 10.1073/pnas.74.2.524Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses findings that DNA sequences specific to Bovine Papilloma Virus (BPV) were found in naturally occurring benign tumors in horses. The study further points out the existence of partial DNA copies of BPV and their thermal stability in the tumors.

Findings of BPV-Specific DNA Sequences in Equine Tumors

  • The research discusses experiments on five spontaneous benign equine connective tissue tumors of unknown cause. It was observed that four of these tumors, along with a tumor induced by BPV, contained BPV-specific DNA sequences.
  • The presence of these sequences was identified through DNA-DNA hybridization. In this process, DNA from the tumors was mixed with BPV DNA labeled in vitro. If the BPV-specific DNA sequences were present in the tumor DNA, the two types of DNA would bind (or hybridize) together, demonstrating their presence.

Analysis of Reassociation and Genome Presence

  • Following further examination into the kinetics of reassociation, it was indicated that between 20% to 75% of the BPV genome was present in the different tumors. This finding suggests that a substantial portion of the virus’s genetic material was replicated within the tumor cells.
  • It was also found that the number of partial equivalents of the BPV genome ranged from 60 to 500 copies per each diploid quantity of cellular DNA, further establishing the presence and replication of the virus DNA into the cellular DNA of the tumors.

Thermal Denaturation of BPV DNA

  • Thermal denaturation profiles of the duplexes formed between the labeled BPV DNA and the tumor cell DNA were analyzed. The study found that two tumors contained viral DNA with base sequences identical to the BPV DNA, and that three tumors (including the one induced by BPV) contained BPV-related DNA sequences which were less stable when exposed to heat.
  • This reduced thermal stability could potentially be due to the presence of adenine and thymine-rich regions of the BPV genome in the tumor cells. Adenine and thymine form weaker bonds in DNA double strands and are more easily separated by heat.

Cite This Article

APA
Lancaster WD, Olson C, Meinke W. (1977). Bovine papilloma virus: presence of virus-specific DNA sequences in naturally occurring equine tumors. Proc Natl Acad Sci U S A, 74(2), 524-528. https://doi.org/10.1073/pnas.74.2.524

Publication

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
NlmUniqueID: 7505876
Country: United States
Language: English
Volume: 74
Issue: 2
Pages: 524-528

Researcher Affiliations

Lancaster, W D
    Olson, C
      Meinke, W

        MeSH Terms

        • Animals
        • Bovine papillomavirus 1 / metabolism
        • DNA, Neoplasm / biosynthesis
        • DNA, Viral / biosynthesis
        • Horses
        • Hot Temperature
        • Kinetics
        • Neoplasms, Experimental / metabolism
        • Nucleic Acid Hybridization
        • Nucleic Acid Renaturation
        • Papillomaviridae / metabolism
        • Temperature

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