Bovine papillomavirus infection in equine sarcoids and in bovine bladder cancers.
Abstract: Bovine papillomavirus (BPV) type 2 is involved in carcinogenesis of the urinary bladder in cattle, while BPV-1 is commonly associated with equine sarcoid tumours. In both cases the early viral proteins are expressed, but virion is not produced. Given the similarities in BPV biology between the tumours in cattle and horses, bovine bladder cancers and equine sarcoids were compared with respect to physical status, load of viral DNA and variability of the E5 open reading frame (ORF). Rolling circle amplification demonstrated that BPV-1 and BPV-2 genomes exist as double stranded, episomal, circular forms in the two tumours. Realtime quantitative PCR revealed that equine sarcoids contained higher viral DNA loads compared to bovine bladder cancers. The BPV-1 E5 ORF showed sequence variation but BPV-2 ORF did not. The presence of BPV-1 E5 variations or their absence in the BPV-2 E5 ORF does not appear to have an effect on viral DNA load in either tumour type.
Publication Date: 2006-12-05 PubMed ID: 17150387DOI: 10.1016/j.tvjl.2006.10.012Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research studied the role of Bovine papillomavirus (BPV) types 1 and 2 in the formation of equine sarcoid tumors and bladder cancers in cattle, respectively. The study revealed that both types exist as double-stranded forms in these tumors, and while equine sarcoids contained higher viral DNA loads, variations in BPV-1 or their absence in BPV-2 did not affect the viral DNA load in either tumor type.
Research Details
- The research is centered on two types of Bovine papillomavirus (BPV): BPV-1, which is linked to equine sarcoid tumors, and BPV-2, which is associated with urinary bladder cancers in cattle. Both types of BPV express early viral proteins, but do not produce any virion, a key factor in these particular forms of carcinogenesis.
Physical Status and Viral Load
- Using a technique called rolling circle amplification, the researchers found that the genomes of BPV-1 and BPV-2 exist as double-stranded, episomal circular forms within the two types of tumors. This indicates the physical presence of the virus in both types of tumors.
- The research team quantified the amount of viral DNA present in the two tumors using real-time PCR. They found that equine sarcoids contained higher viral DNA loads than bovine bladder cancers. This could mean that the virus proliferates more in equine sarcoids as compared to bovine bladder cancers.
Variability of E5 Open Reading Frame (ORF)
- The study also evaluated the variability of the E5 Open Reading Frame (ORF) in BPV-1 and BPV-2. The E5 ORF is an important part of the BPV genome responsible for expressing E5 proteins, which could potentially play a critical role in oncogenesis.
- The researchers discovered sequence variation in the E5 ORF of BPV-1, while no such variation existed in the BPV-2 E5 ORF. Despite these variations or their absence, the viral DNA load in either type of tumor was not affected. This implies that the presence or absence of variations in the E5 ORF does not influence the degree of infection or oncogenesis initiated by the virus.
Cite This Article
APA
Yuan Z, Gallagher A, Gault EA, Campo MS, Nasir L.
(2006).
Bovine papillomavirus infection in equine sarcoids and in bovine bladder cancers.
Vet J, 174(3), 599-604.
https://doi.org/10.1016/j.tvjl.2006.10.012 Publication
Researcher Affiliations
- Division of Pathological Sciences, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, Scotland, UK.
MeSH Terms
- Animals
- Bovine papillomavirus 1 / isolation & purification
- Cattle
- Cattle Diseases / virology
- DNA, Viral / isolation & purification
- Horse Diseases / virology
- Horses
- Plasmids
- Skin Neoplasms / veterinary
- Skin Neoplasms / virology
- Urinary Bladder Neoplasms / veterinary
- Urinary Bladder Neoplasms / virology
Citations
This article has been cited 11 times.- Podstawski P, Ropka-Molik K, Semik-Gurgul E, Samiec M, Skrzyszowska M, Podstawski Z, Szmatoła T, Witkowski M, Pawlina-Tyszko K. Tracking the Molecular Scenarios for Tumorigenic Remodeling of Extracellular Matrix Based on Gene Expression Profiling in Equine Skin Neoplasia Models. Int J Mol Sci 2022 Jun 10;23(12).
- Podstawski P, Samiec M, Skrzyszowska M, Szmatoła T, Semik-Gurgul E, Ropka-Molik K. The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia. Int J Mol Sci 2022 Feb 10;23(4).
- de Alcântara BK, Lunardi M, Agnol AMD, Alfieri AF, Alfieri AA. Detection and Quantification of the E6 Oncogene in Bovine Papillomavirus Types 2 and 13 From Urinary Bladder Lesions of Cattle. Front Vet Sci 2021;8:673189.
- Roperto S, Cutarelli A, Corrado F, De Falco F, Buonavoglia C. Detection and quantification of bovine papillomavirus DNA by digital droplet PCR in sheep blood. Sci Rep 2021 May 13;11(1):10292.
- Podstawski P, Witarski W, Szmatoła T, Bugno-Poniewierska M, Ropka-Molik K. Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid. Animals (Basel) 2020 May 19;10(5).
- Yamashita-Kawanishi N, Tsuzuki M, Wei Z, Kok MK, Ishiyama D, Chambers JK, Uchida K, Dong J, Shimakura H, Haga T. Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas. J Vet Med Sci 2019 Jul 19;81(7):1000-1005.
- Araldi RP, Assaf SMR, Carvalho RF, Carvalho MACR, Souza JM, Magnelli RF, Módolo DG, Roperto FP, Stocco RC, Beçak W. Papillomaviruses: a systematic review. Genet Mol Biol 2017 Jan-Mar;40(1):1-21.
- Trewby H, Ayele G, Borzacchiello G, Brandt S, Campo MS, Del Fava C, Marais J, Leonardi L, Vanselow B, Biek R, Nasir L. Analysis of the long control region of bovine papillomavirus type 1 associated with sarcoids in equine hosts indicates multiple cross-species transmission events and phylogeographical structure. J Gen Virol 2014 Dec;95(Pt 12):2748-2756.
- Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids. J Clin Microbiol 2013 Jul;51(7):2167-71.
- Yuan ZQ, Nicolson L, Marchetti B, Gault EA, Campo MS, Nasir L. Transcriptional changes induced by bovine papillomavirus type 1 in equine fibroblasts. J Virol 2008 Jul;82(13):6481-91.
- Smith CH, Stewart HL, Stefanovski D, Levine DG. Outcomes following autologous tumor tissue implantation with or without concurrent antineoplastic therapies in the treatment of sarcoids in 50 equids. Front Vet Sci 2025;12:1559519.
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