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Journal of clinical microbiology2013; 51(7); 2167-2171; doi: 10.1128/JCM.00371-13

Bovine papillomavirus type 13 DNA in equine sarcoids.

Abstract: Equine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.
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Publication Date: 2013-05-01 PubMed ID: 23637294PubMed Central: PMC3697707DOI: 10.1128/JCM.00371-13Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied equine sarcoids, the most common skin tumors in horses, and found that a newly identified strain of bovine papillomavirus, Bovine Papillomavirus 13, can be associated with these tumors. This expands our understanding of how these tumors might be caused, from previously known types 1 and 2, to include type 13 as well.

Understanding Equine Sarcoids and Bovine Papillomavirus

  • Equine sarcoids are aggressive skin tumors that are prevalent in horses globally. These neoplasms result from uncontrolled growth of cells and are typically associated with Bovine Papillomaviruses (BPV) types 1 and 2.
  • BPV types 1 and 2 were the only known types associated with sarcoids until this research revealed the involvement of BPV type 13, which was previously known only in cattle.

Aim and Methodology of the Research

  • The main objective of the research was to identify if any other strains of BPV, apart from types 1 and 2, could be linked with equine sarcoids.
  • Six sarcoid samples were collected from two horses from different anatomical sites in southern Brazil.
  • A wide range of papilloma virus strains were detected using eight degenerate primer pairs. These primers were designed to bind to and help detect the conserved regions on the L1 and E1 genes of papilloma viruses.
  • The DNA from the amplicons acquired from the primer pairs were directly sequenced. The sequence identities were compared with sequences from all bovine papillomavirus types.

Findings of the Study

  • The sequencing showed that FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences from the sarcoids had 99-100% identity with BPV type 13.
  • This implies that BPV type 13 was present in the sarcoid lesions of the horses, therefore demonstrating that another strain of BPV, apart from types 1 and 2, can be associated with these tumors in horses.

Implications of the Research

  • The research provides valuable insights and broadens the spectrum of BPV types associated with sarcoids. This can potentially improve understanding of the etiology and pathogenesis of equine sarcoids and further contribute to the development of more effective treatment options.
  • The discovery that BPV type 13 can also be involved with equine sarcoids highlights the need for further study to identify the prevalence of this viral type in diverse geographical areas and how it may contribute to these common tumors within the equine population.

Cite This Article

APA
Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. (2013). Bovine papillomavirus type 13 DNA in equine sarcoids. J Clin Microbiol, 51(7), 2167-2171. https://doi.org/10.1128/JCM.00371-13

Publication

Journal of clinical microbiology
ISSN: 1098-660X
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 51
Issue: 7
Pages: 2167-2171

Researcher Affiliations

Lunardi, Michele
  • Laboratory of Veterinary Microbiology, Veterinary Teaching Hospital, Universidade de Cuiaba, Cuiaba, Brazil.
de Alcântara, Brígida Kussumoto
    Otonel, Rodrigo Alejandro Arellano
      Rodrigues, Wagner Borges
        Alfieri, Alice Fernandes
          Alfieri, Amauri Alcindo

            MeSH Terms

            • Animals
            • Brazil
            • DNA, Viral / chemistry
            • DNA, Viral / genetics
            • DNA, Viral / isolation & purification
            • Horse Diseases / virology
            • Horses
            • Molecular Sequence Data
            • Papillomaviridae / classification
            • Papillomaviridae / genetics
            • Papillomaviridae / isolation & purification
            • Sarcoidosis / veterinary
            • Sarcoidosis / virology
            • Sequence Analysis, DNA

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