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Home / Equine Research Bank / BMC Microbiol / Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit ...
BMC microbiology2007; 7; 19; doi: 10.1186/1471-2180-7-19

Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis.

Abstract: The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B. mallei is a recently emerged, host restricted clone of B. pseudomallei. Results: Using bacteriophage-mediated immunoscreening we identified genes expressed in vivo during experimental equine glanders infection. A family of immunodominant antigens were identified that share protein domain architectures with hemagglutinins and invasins. These have been designated Burkholderia Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a B. mallei expression library screened with sera from two infected horses belonged to this family. This contrasted with 6/189 positive clones (3%) of a B. pseudomallei expression library screened with serum from 21 patients with culture-proven melioidosis. Conclusions: Members of the BuHA proteins are found in other Gram-negative bacteria and have been shown to have important roles related to virulence. Compared with other bacterial species, the genomes of both B. mallei and B. pseudomallei contain a relative abundance of this family of proteins. The domain structures of these proteins suggest that they function as multimeric surface proteins that modulate interactions of the cell with the host and environment. Their effect on the cellular immune response to B. mallei and their potential as diagnostics for glanders requires further study.
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Publication Date: 2007-03-15 PubMed ID: 17362501PubMed Central: PMC1847439DOI: 10.1186/1471-2180-7-19Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper revolves around the study of Burkholderia Hep_Hag autotransporter (BuHA) proteins that induce a strong immune response during experimental glanders infection but not in human melioidosis.

Introduction

  • The research revolves around the bacterial biothreat agents, Burkholderia mallei and Burkholderia pseudomallei responsible for glanders and melioidosis respectively.
  • Through genomic and epidemiological analysis, it is established that B. mallei is a recently surfaced, host-restricted clone of B. pseudomallei.

Research Methodology

  • The team used bacteriophage-mediated immunoscreening to identify genes that are expressed in vivo during an experimental equine glanders infection.
  • A dominant family of antigens was identified which shares protein domain structures with hemagglutinins and invasins. They are designated as Burkholderia Hep_Hag autotransporter (BuHA) proteins.

Results

  • The study found that 110 out of 207 positive clones (approximately 53%) of a B. mallei expression library tested with sera from two infected horses belonged to the BuHA family.
  • In contrast, just 6 out of 189 positive clones (around 3%) of a B. pseudomallei expression library tested with serum from 21 patients with proven melioidosis were the part of this family.

Conclusions

  • BuHA proteins are also found in other Gram-negative bacteria, and have a significant role in relation to virulence.
  • Compared to other bacterial species, the genomes of B. mallei and B. pseudomallei have a relative abundance of this protein family.
  • The domain structures of these proteins might function as multimeric surface proteins that affect the interactions of the cell with the host and the environment.
  • The proteins’ influence on the cellular immune response to B. mallei and their potential as diagnostic tools for glanders needs further study.

Cite This Article

APA
Tiyawisutsri R, Holden MT, Tumapa S, Rengpipat S, Clarke SR, Foster SJ, Nierman WC, Day NP, Peacock SJ. (2007). Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis. BMC Microbiol, 7, 19. https://doi.org/10.1186/1471-2180-7-19

Publication

BMC microbiology
ISSN: 1471-2180
NlmUniqueID: 100966981
Country: England
Language: English
Volume: 7
Pages: 19

Researcher Affiliations

Tiyawisutsri, Rachaneeporn
  • Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. rachaneeporn_t@hotmail.com
Holden, Matthew T G
    Tumapa, Sarinna
      Rengpipat, Sirirat
        Clarke, Simon R
          Foster, Simon J
            Nierman, William C
              Day, Nicholas P J
                Peacock, Sharon J

                  MeSH Terms

                  • Adolescent
                  • Adult
                  • Aged
                  • Animals
                  • Antibodies, Bacterial / biosynthesis
                  • Antibodies, Bacterial / immunology
                  • Antigens, Bacterial / biosynthesis
                  • Antigens, Bacterial / genetics
                  • Antigens, Bacterial / immunology
                  • Bacterial Outer Membrane Proteins / biosynthesis
                  • Bacterial Outer Membrane Proteins / genetics
                  • Bacterial Outer Membrane Proteins / immunology
                  • Bacterial Proteins / biosynthesis
                  • Bacterial Proteins / genetics
                  • Bacterial Proteins / immunology
                  • Base Sequence
                  • Burkholderia mallei / genetics
                  • Burkholderia mallei / immunology
                  • Burkholderia pseudomallei / genetics
                  • Burkholderia pseudomallei / immunology
                  • Child
                  • Female
                  • Gene Library
                  • Glanders / diagnosis
                  • Glanders / immunology
                  • Glanders / microbiology
                  • Hemagglutinins / immunology
                  • Horses
                  • Humans
                  • Male
                  • Melioidosis / blood
                  • Melioidosis / immunology
                  • Melioidosis / microbiology
                  • Middle Aged
                  • Molecular Sequence Data
                  • Virulence Factors / biosynthesis
                  • Virulence Factors / genetics
                  • Virulence Factors / immunology

                  Grant Funding

                  • Wellcome Trust

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