Calcium Electroporation of Equine Sarcoids.
Abstract: Sarcoids are common equine skin tumors where the risk of recurrence after treatment is high, and better treatment options are warranted. Calcium electroporation is a novel anti-cancer treatment where lethally high calcium concentrations are introduced into the cells by electroporation, a method where short high-voltage pulses induce transient permeabilization of the cell membrane. This study investigated the safety and long-term response of calcium electroporation on sarcoids. Thirty-two sarcoids in eight horses were included. The study suggested that calcium electroporation is a safe and feasible treatment for sarcoids, including inoperable sarcoids. Horses were treated once (2/8) or twice (6/8) under general anesthesia, where sarcoids were injected with 220 mM calcium chloride followed by electroporation with 8 pulses of 100 μs, 1 kV/cm, and 1 Hz. Biopsies were taken prior to treatment. The sarcoid size was monitored for 12-38 weeks after the first treatment. Complete response was observed in 22% (6/27) of treated sarcoids, and partial response in 22% (6/27), giving a 44% total response. Treatment efficacy did not appear to be related to location, type, or size. In all non-biopsied lesions, a complete response was seen (4/4). In conclusion, in this small study, 44% of sarcoids responded with 22% of sarcoids disappearing.
Publication Date: 2020-03-19 PubMed ID: 32204512PubMed Central: PMC7143334DOI: 10.3390/ani10030517Google Scholar: Lookup
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- Journal Article
Summary
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This study investigates the effectiveness of a new anti-cancer treatment, calcium electroporation, on equine skin tumors known as sarcoids. The research found that 44% of treated sarcoids responded to the treatment, with 22% completely disappearing.
Background of the Study
- Sarcoids are prevailingly occurring skin tumors in horses. The tumors have a high risk of recurrence after treatment, necessitating more effective treatment options.
- This investigation centers on calcium electroporation, a new anti-cancer treatment method. The procedure involves introducing lethally high concentrations of calcium into cells through electroporation, a technique that uses short high-voltage pulses to temporarily permeabilize the cell membrane.
- The aim of the study was to assess the safety and long-term effectiveness of calcium electroporation on sarcoids.
Methodology
- The study included 32 sarcoids from eight horses. The response and safety of the treatment were evaluated.
- Sarcoids received an injection of 220 mM calcium chloride, followed by eight electroporation pulses, each comprising 100 μs, 1 kV/cm, and 1 Hz. The horses received treatment once (2/8) or twice (6/8) under general anesthesia.
- Tumor size was monitored from 12 to 38 weeks after the initial treatment, and biopsies were taken prior to the treatment for evaluation.
Findings
- The study noted a total response rate of 44% among treated sarcoids, with partial and complete responses each accounting for 22% of the total. In all cases where lesions were not biopsied, a complete response was observed.
- Importantly, the effectiveness of treatment did not appear to be associated with the location, type, or size of the sarcoid. This indicates the general applicability of this method across different tumor attributes.
- In conclusion, the study suggested calcium electroporation could be a safe and viable treatment for horse sarcoids, including those that are inoperable.
Cite This Article
APA
Frandsen SK, Gehl J, Tramm T, Thoefner MS.
(2020).
Calcium Electroporation of Equine Sarcoids.
Animals (Basel), 10(3).
https://doi.org/10.3390/ani10030517 Publication
Researcher Affiliations
- Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Clinical Oncology and Palliative Care, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark.
- Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Clinical Oncology and Palliative Care, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark.
- Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
- Department of Pathology, Aarhus University Hospital, Palle Juul-Jensens Boulevard, 8200 Aarhus N, Denmark.
- Hørsholm Hestepraksis, Kongevejen 124, 3480 Fredensborg, Denmark.
Grant Funding
- 23.06.2018 J. Gehl / Savvaerksejer Jeppe Juhl og Hustru Ovita Juhls Mindelegat
Conflict of Interest Statement
A patent, “Therapeutic applications of calcium electroporation to effectively induce tumor necrosis”, has been granted and licensed (co-inventors S.K. Frandsen and J. Gehl). The sponsors had no role in the design, execution, interpretation, or writing of the study.
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