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Animal reproduction2019; 16(2); 340-347; doi: 10.21451/1984-3143-AR2019-0039

Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion?

Abstract: The objective of this study was to compare the BotuCrio extender with the Merk - egg yolk and the INRA 82 modified by the inclusion of acetamide, methyl cellulose and trehalose in substitution of glycerol for freezing equine semen. The semen was diluted after centrifugation to obtain 100 x 10 of sperm/ml in: BotuCrio (control); Merk - egg yolk or INRA 82 modified (Experiment 1). The extended semen was packaged in 0.5 ml straws, cooled and frozen in a freezing machine. The control extender was superior in preserving the motility, VCL, VSL, VAP, LIN, STR and the BCF when compared to the Merk - egg yolk and INRA 82 modified (P < 0.05). The BotuCrio® preserved more effectively the equine sperm viability characteristics evaluated in Experiment 1 and was used as a control extender in Experiment 2 to test the effectiveness of using LDL in replacement of egg yolk. BotuCrio was superior to preserve progressive motility, VCL, VSL, VAP, LIN, STR and the percentage of functional integrity of sperm membranes compared to BotuCrio LDL (P 0.05). The fertility rate after AI with frozen semen in BotuCrio LDL was 37.5%.
Copyright © The Author(s). Published by CBRA.
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Publication Date: 2019-10-23 PubMed ID: 33224296PubMed Central: PMC7673584DOI: 10.21451/1984-3143-AR2019-0039Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to examine the efficiency of using LDL (low-density lipoprotein), instead of egg yolk, in the BotuCrio® extender to preserve sperm from Mangalarga Marchador stallions. The study confirmed that while both LDL and the traditional egg yolk technique maintained similar overall sperm health, the traditional BotuCrio® extender showed superiority concerning specific sperm mobility and percentages of functional sperm membrane integrity.

Context and Methodology

  • The research was primarily focused on identifying better methods to preserve stallion sperm, specifically from the Mangalarga Marchador breed.
  • The extender compounds used were BotuCrio®, Merk – egg yolk, and a modified INRA 82. Out of these, BotuCrio® was marked as the control.
  • The comparative study was divided into two experiments; Experiment 1 compared BotuCrio®, Merk – egg yolk, and the modified INRA 82, while experiment 2 compared the effectiveness of BotuCrio® with LDL in place of egg yolk.
  • The semen was diluted, extended, packaged, cooled, and frozen in a freezing machine for assessment.

Findings of Experiment 1

  • The traditional BotuCrio® extender was found to be more effective than both Merk – egg yolk and the modified INRA 82 in preserving several features of the semen.
  • These features included sperm motility, VCL (track speed), VSL (straight line velocity), VAP (path velocity), LIN (linearity), STR (straightness), and BCF (beat cross frequency).
  • The success of BotuCrio® in Experiment 1 led to its use as a control in Experiment 2.

Findings of Experiment 2

  • When LDL was used as an alternative to egg yolk in BotuCrio®, it showed similar results in maintaining total sperm motility, ALH (amplitude of lateral head displacement), BCF, and the structural integrity of the membranes.
  • However, the traditional BotuCrio® was still superior in preserving progressive sperm motility, VCL, VSL, VAP, LIN, STR, and the percentage of functional integrity of sperm membranes.
  • The fertility rate with semen frozen in BotuCrio® LDL was found to be 37.5%.

Conclusion

  • The study suggests while LDL can maintain overall sperm health similarly as the egg yolk technique, the traditional BotuCrio® extender is superior in maintaining specific mobility characteristics and a higher percentage of functional sperm membrane integrity.

Cite This Article

APA
Snoeck PPDN, Pessoa THO, Pereira MGS, Bastos ICL, de Melo MIV. (2019). Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion? Anim Reprod, 16(2), 340-347. https://doi.org/10.21451/1984-3143-AR2019-0039

Publication

Animal reproduction
ISSN: 1984-3143
NlmUniqueID: 101272804
Country: Brazil
Language: English
Volume: 16
Issue: 2
Pages: 340-347

Researcher Affiliations

Snoeck, Paola Pereira das Neves
  • Universidade Estadual de Santa Cruz, Ciências Agrárias e Ambientais, Ilheus, Bahia, Brazil.
Pessoa, Tainá Hortência Oliveira
  • Universidade Estadual de Santa Cruz, Ciências Agrárias e Ambientais, Ilheus, Bahia, Brazil.
Pereira, Marcelo Gonçalves Sousa
  • Universidade Estadual de Santa Cruz, Ciências Agrárias e Ambientais, Ilheus, Bahia, Brazil.
Bastos, Inara Cristina Leal
  • Universidade Estadual de Santa Cruz, Ciências Agrárias e Ambientais, Ilheus, Bahia, Brazil.
de Melo, Maria Isabel Vaz
  • Pontífica Universidade Católica de Minas Gerais, Medicina Veterinária, Belo Horizonte, Brazil.

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Citations

This article has been cited 1 times.
  1. Ataei-Nazari S, Benko F, Slanina T, Vozaf J, Ďuračka M, Války T, Chrenek P, Tvrdá E, Kačániová M. Type of Extender and Equilibration Time as Factors Affecting Post-Thaw Quality Characteristics of Turkey Semen. Animals (Basel) 2025 Nov 5;15(21).
    doi: 10.3390/ani15213218pubmed: 41227546google scholar: lookup
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