Candidatus Rickettsia andeanae, a spotted fever group agent infecting Amblyomma parvum ticks in two Brazilian biomes.
Abstract: Adult ticks of the species Amblyomma parvum were collected from the vegetation in the Pantanal biome (state of Mato Grosso do Sul) and from horses in the Cerrado biome (state of Piauí) in Brazil. The ticks were individually tested for rickettsial infection via polymerase chain reaction (PCR) targeting three rickettsial genes, gltA, ompA and ompB. Overall, 63.5% (40/63) and 66.7% (2/3) of A. parvum ticks from Pantanal and Cerrado, respectively, contained rickettsial DNA, which were all confirmed by DNA sequencing to be 100% identical to the corresponding fragments of the gltA, ompA and ompB genes of Candidatus Rickettsia andeanae. This report is the first to describe Ca. R. andeanae in Brazil.
Publication Date: 2014-04-10 PubMed ID: 24714968PubMed Central: PMC4015251DOI: 10.1590/0074-0276140283Google Scholar: Lookup
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Summary
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This research investigates the infection of the tick species Amblyomma parvum by the bacteria Candidatus Rickettsia andeanae in two different areas in Brazil. The study confirmed this infection through polymerase chain reaction (PCR) tests and DNA sequencing.
Objective and Methodology
- The research sought to investigate the presence of Candidatus Rickettsia andeanae, a type of bacteria, in the tick species Amblyomma parvum.”);
- These ticks were collected from two different biomes in Brazil: the Pantanal biome from vegetation and the Cerrado biome from horses.”);
- In order to test for rickettsial infection, the researchers performed a polymerase chain reaction (PCR), which is a method used to amplify select sections of DNA.
- The PCR test was targeting three specific rickettsial genes, namely gltA, ompA, and ompB.
Results and Findings
- The PCR tests revealed that 63.5% (40 out of 63) A. parvum ticks from the Pantanal biome and 66.7% (2 out of 3) ticks from the Cerrado biome were infected with rickettsial DNA.
- This DNA was sequenced and confirmed to be identical to the gltA, ompA, and ompB genes of Ca. R. andeanae.
- This finding is significant as it is the first report of Ca. R. andeanae in Brazil, indicating that it may be found in biomes across Brazil.
Implications and Significance
- The presence of Ca. R. andeanae in A. parvum ticks in Brazil is of importance to both public health and veterinary medicine.
- Understanding the presence and prevalence of tick-borne diseases can help in creating prevention and treatment protocols.
- This study adds to the body of knowledge regarding the distribution and range of Ca. R. andeanae, which can help in tracking and managing the spread of this bacterium.
Cite This Article
APA
Nieri-Bastos FA, Lopes MG, Cançado PH, Rossa GA, Faccini JL, Gennari SM, Labruna MB.
(2014).
Candidatus Rickettsia andeanae, a spotted fever group agent infecting Amblyomma parvum ticks in two Brazilian biomes.
Mem Inst Oswaldo Cruz, 109(2), 259-261.
https://doi.org/10.1590/0074-0276140283 Publication
Researcher Affiliations
- Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
- Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
- Embrapa Gado de Corte, Campo Grande, MS, Brasil.
- Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
- Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brasil.
- Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
- Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
MeSH Terms
- Animals
- Arachnid Vectors / microbiology
- Brazil
- Ecosystem
- Female
- Horses / parasitology
- Ixodidae / microbiology
- Male
- Polymerase Chain Reaction
- Rickettsia / genetics
- Rickettsia / isolation & purification
- Sequence Analysis, DNA
- Tick-Borne Diseases / diagnosis
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